Skip to main content

Advertisement

SpringerLink
Log in

Studies on the influence of DNA repair on radiosensitivity in prostate cell lines

  • Original Paper
  • Published:
Urological Research Aims and scope Submit manuscript

Abstract

The relationship between radiosensitivity and DNA repair was investigated in six human prostate cell lines, 1542-NPTX, BPH-1, 1542-CP3TX, 1532-CP2TX, 1535-CP1TX and LNCaP. Except for LNCaP, these cell lines are new and were derived from primary prostate tumours and normal non-tumourigenic prostate tissue. Cell survival was assessed by clonogenic assay. DNA damage was determined in non-synchronised cells by constant-field gel electrophoresis, and expressed as the fraction of DNA released. For initial damage, cells were embedded in agarose and irradiated on ice with 0–100 Gy 60Co γ-irradiation. Residual DNA damage was measured after 2 h and 20 h of repair. Radiosensitivity, given as the mean inactivation dose, was found to vary between 1.62 and 2.77 Gy. We found that radiosensitivity significantly correlates with the 2 h DNA repair component, giving a correlation coefficient of 0.92 (P=0.009). In the cell lines examined here the 2 h repair component emerges as an indicator of radiosensitivity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

References

  1. Akudugu JM, Slabbert JP, Serafin A, Bohm L (2000) Frequency of radiation-induced micronuclei in neuronal cells does not correlate with clonogenic survival. Radiat Res 153: 62

    CAS  PubMed  Google Scholar 

  2. Arrand JE, Michael BD (1992) Recent advances in the study of ionizing radiation damage and repair. Int J Radiat Biol 61: 717

    CAS  PubMed  Google Scholar 

  3. Bayne CW, Ross M, Donnelly F, Chapman K, Buck C, Bollina P, Habib FK (1998) Selective interactions between prostate fibroblasts and epithelial cells in co-culture maintain the BPH phenotype. Urol Int 61: 1

    CAS  PubMed  Google Scholar 

  4. Bright RK, Vocke CD, Emmert-Buck MR, Duray PH, Solomon D, Fetsch P, Rhim JS, Linehan WM, Topalian SL (1997) Generation and genetic characterization of immortal human prostate epithelial cell lines derived from primary cancer specimens. Cancer Res 57: 995

    CAS  PubMed  Google Scholar 

  5. Bristow RG, Benchimol S, Hill RP (1996) The p53 gene as a modifier of intrinsic radiosensitivity: implications for radiotherapy. Radiother Oncol 40: 197

    Article  CAS  PubMed  Google Scholar 

  6. Britten RA, Murray D (1997) Constancy of the relative biological effectiveness of 42 MeV (p→Be+) neutrons among cell lines with different DNA repair proficiencies. Radiat Res 148: 308

    CAS  PubMed  Google Scholar 

  7. deKernion J, Belldegrun A, Naitoh J (1998) Surgical treatment of localized prostate cancer: indications, techniques and results. In: Belldegrun A, Kirby RS, Oliver T (eds) New perspectives in prostate cancer. Oxford University Press, Oxford, p 189

  8. Dikomey E, Dahm-Daphi J, Brammer I, Martensen R, Kaina B (1998) Correlation between cellular radiosensitivity and non-repaired double strand breaks studied in nine mammalian cell lines. Int J Radiat Biol 73: 269

    CAS  PubMed  Google Scholar 

  9. Fertil B, Dertinger H, Courdi A, Malaise EP (1984) Mean inactivation dose: a useful concept for intercomparison of human cell survival curves. Radiat Res 99: 73

    CAS  PubMed  Google Scholar 

  10. Frankenberg-Schwager M (1989) Review of repair kinetics for DNA damage induced in eukaryotic cells in vitro by ionizing radiation. Radiother Oncol 14: 307

    Google Scholar 

  11. Han M, Partin AW (2001) Anatomic radical retropubic prostatectomy for prostate cancer. In: Chung LWK, Isaacs WB, Simons JW (eds) Prostate cancer: biology, genetics, and the new therapeutics. Humana Press, New Jersey, p 386

    Google Scholar 

  12. Hayward SW, Dahiya R, Cunha GR, Bartek J, Deshpande N, Narayan P (1995) Establishment and characterization of an immortalised but non-transformed human prostate epithelial cell line: BPH-1. In Vitro Cell Dev Biol 31A: 14

    Google Scholar 

  13. Horoszewicz JS, Leong SS, Kawinski E, Karr JP, Rosenthal H, Ming Chu T, Mirand EA, Murphy GP (1983) LNCaP model of human prostatic carcinoma. Cancer Res 43:1809

    CAS  PubMed  Google Scholar 

  14. Karran P (2000) DNA double strand break repair in mammalian cells. Curr Opin Genet Dev 10: 144

    CAS  PubMed  Google Scholar 

  15. Kysela BP, Michael BD, Arrand JE (1993) Relative contributions of initial DNA damage and repair of double strand breaks to the ionising radiation-sensitive phenotype of the Chinese hamster cell mutant, XR–V15B. Part 1: X–rays. Int J Radiat Biol 63: 609

    CAS  PubMed  Google Scholar 

  16. Lee JM, Bernstein A (1995) Apoptosis, cancer and the p53 tumour suppressor gene. Cancer Metastasis Rev 14: 149

    CAS  PubMed  Google Scholar 

  17. Maity A, Kao GD, Muschel RJ, McKenna WG (1997) Potential molecular targets for manipulating the radiation response. Int J Radiat Oncol Biol Phys 37: 639

    Article  CAS  PubMed  Google Scholar 

  18. McMillan TJ, Eady JJ, Holmes A, Peacock JH (1989) The radiosensitivity of human neuroblastoma: a cellular and molecular study. Int J Radiat Biol 56: 651

    CAS  PubMed  Google Scholar 

  19. Morgan SE, Kastan MB (1997) p53 and ATM: cell cycle, cell death, and cancer. Adv Cancer Res 71: 1

    CAS  PubMed  Google Scholar 

  20. Nunez MI, McMillan TJ, Valenzuela MT, Ruiz de Almodovar JM, Pedraza V (1996) Relationship between DNA damage, rejoining and cell killing by radiation in mammalian cells. Radiother Oncol 39: 155

    Article  CAS  PubMed  Google Scholar 

  21. Olive PL, Banath JP, MacPhail HS (1994) Lack of a correlation between radiosensitivity and DNA double-strand break induction or rejoining in six human tumor cell lines. Cancer Res 54: 3939

    CAS  PubMed  Google Scholar 

  22. Raqde H, Elqamal AA, Snow PB, Brandt J, Bartolucci AA, Nadir BS, Korb LJ (1998) Ten-year disease free survival after transperineal sonography-guided iodine-125 brachytherapy with or without 45-gray external beam irradiation in the treatment of patients with clinically localized, low to high Gleason grade prostate carcinoma. Cancer 83: 989

    Article  PubMed  Google Scholar 

  23. Roos WP, Binder A, Bohm L (2000) Determination of the initial DNA damage and residual DNA damage remaining after 12 hours of repair in eleven cell lines at low doses of irradiation. Int J Radiat Biol 76: 1493

    CAS  PubMed  Google Scholar 

  24. Ruiz de Almodovar JM, Nunez MI, McMillan TJ, Olea N, Mort C, Villalobos M, Pedraza V, Steel GG (1994) Initial radiation-induced DNA damage in human tumour cell lines: a correlation with intrinsic cellular radiosensitivity, Br J Cancer 69: 457

    Google Scholar 

  25. Schwartz JL, Rotmensch J, Giovanazzi S, Cohen MB, Weichselbaum RR (1988) Faster repair of DNA double-strand breaks in radioresistant human tumor cells, Int J Radiat Oncol Biol Phys 15: 907

    CAS  PubMed  Google Scholar 

  26. Schwartz JL, Mustafi R, Beckett MA, Weichselbaum RR (1990) Prediction of the sensitivity of human squamous cell carcinoma cells using DNA filter elution. Radiat Res 123: 1

    CAS  PubMed  Google Scholar 

  27. Serafin AM, Binder AB, Bohm L (2001) Chemosensitivity of prostatic tumour cell lines under conditions of G2 block abrogation. Urol Res 29: 221

    CAS  PubMed  Google Scholar 

  28. Smeets MFMA, Mooren EHM, Begg AC (1993) Radiation-induced DNA damage and repair in radiosensitive and radioresistant human tumour cells measured by field inversion gel electrophoresis. Int J Radiat Biol 63: 703

    CAS  PubMed  Google Scholar 

  29. Theron T, Binder A, Verheye-Dua F, Bohm L (2000) The role of G2–block abrogation, DNA double-strand break repair and apoptosis in the radiosensitisation of melanoma and squamous cell carcinoma cell lines by pentoxifylline. Int J Radiat Biol 76: 1197

    Article  CAS  PubMed  Google Scholar 

  30. Weichselbaum RR, Beckett MA, Schwartz JL, Dritschilo A (1988) Radioresistant tumor cells are present in head-and-neck carcinomas that recur after radiotherapy, Int J Radiat Oncol Biol Phys 14: 575

    Google Scholar 

  31. Whitmore WF (1984) Irradiation and/or surgery: some areas of confrontation in urologic oncology. Am J Clin Oncol 7: 606

    Google Scholar 

  32. Wlodek D, Banath J, Olive PL (1991) Comparison between pulsed field and constant field electrophoresis for measurement of DNA double-strand breaks in irradiated Chinese hamster ovary cells. Int J Radiat Biol 60: 779

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the Cancer Association of SA (CANSA), and the David and Freda Becker Trust to L.B.

Author information

Authors and Affiliations

  1. Department of Radiation Oncology, Radiobiology Laboratory, Faculty of Health Sciences and Tygerberg Hospital, PO Box 19063, Tygerberg 7505, South Africa

    Antonio M. Serafin, John M. Akudugu & Lothar Böhm

Authors
  1. Antonio M. Serafin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John M. Akudugu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lothar Böhm
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lothar Böhm.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Serafin, A.M., Akudugu, J.M. & Böhm, L. Studies on the influence of DNA repair on radiosensitivity in prostate cell lines. Urol Res 31, 227–231 (2003). https://doi.org/10.1007/s00240-003-0299-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00240-003-0299-0

Keywords

Search

Navigation