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Alternative DNA amplification methods to PCR and their application in GMO detection: a review

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Abstract

Nucleic acids, especially DNA, are targets of qualitative and quantitative diagnostics for genetically modified organisms (GMO) in seeds, food- and feedstuff. The amplification of the nucleic acid is an essential step for further analyses of the target sequence. The PCR has been the method of choice for DNA amplification in most laboratories, and its real-time version (qPCR) also enables quantitative analysis of target contents. Despite its numerous advantages, PCR technology has some limitations such as the lack of true multiplexing properties. To alleviate the drawbacks linked to PCR technology, alternative nucleic acid amplification methods with promising characteristics are being developed fast. These methods, their advantages, and the inconveniences, which are not yet resolved are summarized in the paper. Special focus is given to the possibilities of using these alternative methods for GMO detection in future, when expansion of GMOs both in diversity and frequencies will make current GMO detection systems difficult to operate.

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Acknowledgments

The authors thank Dr Mike Galsworthy for critical review of the manuscript. This study was supported by the Slovenian Ministry of Higher Education, Science and Technology (P4-0165) and the European Commission’s Sixth Framework Program through the integrated project Co-extra (contract no. 7158).

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Authors and Affiliations

  1. Department of Plant Physiology and Biotechnology, National Institute of Biology, Vecna pot 111, 1000, Ljubljana, Slovenia

    Dany Morisset, Dejan Stebih, Katarina Cankar, Jana Zel & Kristina Gruden

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  1. Dany Morisset
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  2. Dejan Stebih
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Correspondence to Dany Morisset.

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Morisset, D., Stebih, D., Cankar, K. et al. Alternative DNA amplification methods to PCR and their application in GMO detection: a review. Eur Food Res Technol 227, 1287–1297 (2008). https://doi.org/10.1007/s00217-008-0850-x

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  • DOI: https://doi.org/10.1007/s00217-008-0850-x

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