Abstract
The red imported fire ant, Solenopsis invicta, is an aggressive, highly invasive pest ant species from South America that has been introduced into North America, Asia, and Australia. Quarantine efforts have been imposed in the USA to minimize further spread of the ant. To aid the quarantine efforts, there remains an acute need for a rapid, field portable method for the identification of these ants. In this report, we describe two novel monoclonal antibodies that specifically bind the S. invicta venom protein 2 produced by S. invicta. Using these monoclonal antibodies we developed a lateral flow immunoassay that provides a rapid and portable method for the identification of S. invicta ants. The lateral flow immunoassay was validated against purified S. invicta venom protein 2 and 33 unique ant species (representing 15 % of the total species and 42 % of the Myrmicinae genera found in Florida), and only S. invicta and the S. invicta/richteri hybrid produced a positive result. These monoclonal antibodies were selective to S. invicta venom protein 2 and did not bind to proteins from congeners (i.e., S. geminata or S. richteri) known to produce a S. invicta venom protein 2 ortholog. This S. invicta lateral flow immunoassay provides a new tool for regulatory agencies in the USA to enforce quarantine protocols and limit the spread of this invasive ant.
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References
Tschinkel WR. The fire ants. Cambridge: The Belknap Press of Harvard University Press; 2006.
Pereira RM. Areawide suppression of fire ant populations in pastures: project update. J Agric Urban Entomol. 2003;20(2):123–30.
Callcott AM, Collins HL. Invasion and range expansion of imported fire ants (Hymenoptera: Formicidae) in North America from 1918–1995. Fla Entomol. 1996;79(2):240–51.
Streett DA, Freeland TB, Vander Meer RK. Survey of imported fire ant (Hymenoptera : Formicidae) populations in Mississippi. Fla Entomol. 2006;89(1):91–2.
Bertagnolli D, Graham LC, Vander Meer RK. Ouch! Who bit me? IFA in Alabama. In: 2007 annual imported fire ant conference. 2007. p. 112–115.
Oliver JB, Vander Meer RK, Ochieng SA, Youssef NN, Hosack MY, Mrema FA, et al. Statewide survey of imported fire ant (Hymenoptera: Formicidae) populations in Tennessee. J Entomol Sci. 2009;44:149–57.
Williams DF, Collins HL, Oi DH. The red imported fire ant (Hymenoptera: Formicidae): an historical perspective of treatment programs and the development of chemical baits for control. Am Entomol. 2001;47(3):146–59.
Stablein JJ, Lockey RF. Adverse reactions to ant stings. Clin Rev Allergy. 1987;5(2):161–75.
Porter SD, Oi DH, Valles SM, Vander Meer RK. Mitigating the allergic effects of fire ant envenomation with biologically based population reduction. Curr Opin Allergy Clin Immunol. 2013;13(4):372–8.
Valles SM, Porter SD. Identification of polygyne and monogyne fire ant colonies (Solenopsis invicta) by multiplex PCR of Gp-9 alleles. Insect Soc. 2003;50(2):199–200.
Ross KG, Shoemaker DD. Species delimitation in native South American fire ants. Mol Ecol. 2005;14(11):3419–38.
Hoffman DR. Ant venoms. Curr Opin Allergy Clin Immunol. 2010;10(4):342–6.
Schmidt M, Walker RB, Hoffman DR, Mcconnell TJ. Cloning and sequencing of the cDNA-encoding the imported fire ant venom allergen, SoliII. J Allergy Clin Immunol. 1993;91(1):186.
Hoffman DR. Allergens in hymenoptera venom: the amino acid sequences of imported fire ant venom allergens SoliII, SoliIII, and SoliIV. J Allergy Clin Immunol. 1993;91(1):71–8.
Hopp TP, Woods KR. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci Biol. 1981;78(6):3824–8.
Fisher BL, Cover SP. Ants of North America, a guide to the genera. Berkeley: University of California Press; 2007.
MacGown JA (2003) Ants (Formicidae) of the Southeastern United States. Mississippi State University. www.mississippientomologicalmuseum.org. Accessed 2015.
Deyrup M, Cover S. A new species of Odontomachus ant (Hymenoptera : Formicidae) from inland ridges of Florida, with a key to Odontomachus of the United States. Fla Entomol. 2004;87(2):136–44.
Trager JC, MacGown JA, Trager MD. Revision of the nearctic endemic Formica pallidefulva group. In: Snelling RR, Fisher BL, Ward PS, editors. Advances in ant systematics (Hymenoptera: Formicidae): homage to E. O. Wilson–50 years of contributions. Plazi.org. 2007.
Rabeling C, Cover SP, Johnson RA, Mueller UG. A review of the North American species of the fungus-gardening ant genus Trachymyrmex (Hymenoptera: Formicidae). Zootaxa. 2007;1664:1–53.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680–5.
O'Farrell B. Lateral flow immunoassay systems: evolution from the current state of the art to the next generation of highly sensitive, quantitative rapid assays. In: Wild D, editor. The immunoassay handbook. Oxford: Elsevier; 2013. p. 89–107.
Choi S, Lee JH, Choi JS, Jung HI. Economical and rapid manufacturing of lateral flow immunosensor using fountain pens and gold colloidal solution. Anal Methods. 2015;7(5):1834–42.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997;25(17):3389–402.
Borer AS, Wassmann P, Schmidt M, Hoffman DR, Zhou J-J, Wright C, et al. Crystal structure of Sol i 2: a major allergen from fire ant venom. J Mol Biol. 2012;415(4):635–48.
Hoffman DR. Reactions to less common species of fire ants. J Allergy Clin Immunol. 1997;100(5):679–83.
Haight KL, Tschinkel WR. Patterns of venom synthesis and use in the fire ant, Solenopsis invicta. Toxicon. 2003;42(6):673–82.
Baer H, Liu TY, Anderson MC, Blum M, Schmid WH, James FJ. Protein components of fire ant venom (Solenopsis invicta). Toxicon. 1979;17(4):397–405.
Hoffman DR. Fire ant venom allergy. Allergy. 1995;50(7):535–44.
Deyrup M. An updated list of Florida ants (Hymenoptera: Formicidae). Fla Entomol. 2003;86(1):43–8.
Acknowledgments
We thank D. Oi (USDA-ARS, Gainesville, FL) and R. Hnasko (USDA-ARS, Albany, CA) for critical reviews of the manuscript and R. Hnasko for valuable discussions on antibodies and LFA technique. We also thank Dr. J. Oliver (Tennessee State University) for providing the Solenopsis richteri samples. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. This material was made possible, in part, by a Cooperative Agreement from the United States Department of Agriculture’s Animal and Plant Health Inspection Service (APHIS) and may not necessarily express the views of APHIS.
Author contributions
Conceived and designed the experiments: S.M. Valles. Performed the experiments: S.M. Valles, C.A. Strong. Analyzed the data: S. M. Valles, C.A. Strong. Wrote the paper: S. M. Valles, A-M.A Callcott.
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Antibody production was completed under contract by ProMab Biotechnologies in compliance with state and federal laws.
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Valles, S.M., Strong, C.A. & Callcott, AM.A. Development of a lateral flow immunoassay for rapid field detection of the red imported fire ant, Solenopsis invicta (Hymenoptera: Formicidae). Anal Bioanal Chem 408, 4693–4703 (2016). https://doi.org/10.1007/s00216-016-9553-5
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DOI: https://doi.org/10.1007/s00216-016-9553-5