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A novel application of microwave-assisted extraction of polyphenols from brewer’s spent grain with HPLC-DAD-MS analysis

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Abstract

This paper reports a novel application of microwave-assisted extraction (MAE) of polyphenols from brewer’s spent grains (BSG). A 24 orthogonal composite design was used to obtain the optimal conditions of MAE. The influence of the MAE operational parameters (extraction time, temperature, solvent volume and stirring speed) on the extraction yield of ferulic acid was investigated through response surface methodology. The results showed that the optimal conditions were 15 min extraction time, 100 °C extraction temperature, 20 mL of solvent, and maximum stirring speed. Under these conditions, the yield of ferulic acid was 1.31 ± 0.04% (w/w), which was fivefold higher than that obtained with conventional solid–liquid extraction techniques. The developed new extraction method considerably reduces extraction time, energy and solvent consumption, while generating fewer wastes. HPLC-DAD-MS analysis indicated that other hydroxycinnamic acids and several ferulic acid dehydrodimers, as well as one dehydrotrimer were also present, confirming that BSG is a valuable source of antioxidant compounds.

Response surface of FA yield (Y, %, w/w) as a function of extraction time (X 1) and the other variables studied: a solvent volume (X 3); b temperature (X 2)

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Acknowledgments

This work has been supported by Fundação para a Ciência e a Tecnologia (FCT) through grant no. PEst-C/EQB/LA0006/2011. M.M.M. wishes to acknowledge FCT for her PhD studentship (SFRH/BD/60577/2009). The authors also thank UNICER – Bebidas de Portugal for their support, including the supply of brewer’s spent grain samples.

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Authors and Affiliations

  1. REQUIMTE—Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre 687, 4169-007, Porto, Portugal

    Manuela M. Moreira, Aquiles A. Barros & Luís F. Guido

  2. REQUIMTE—Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072, Porto, Portugal

    Simone Morais & Cristina Delerue-Matos

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  1. Manuela M. Moreira
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  2. Simone Morais
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  3. Aquiles A. Barros
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  4. Cristina Delerue-Matos
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  5. Luís F. Guido
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Correspondence to Luís F. Guido.

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Published in the special issue Euroanalysis XVI (The European Conference on Analytical Chemistry) with guest editor Slavica Ražić.

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Moreira, M.M., Morais, S., Barros, A.A. et al. A novel application of microwave-assisted extraction of polyphenols from brewer’s spent grain with HPLC-DAD-MS analysis. Anal Bioanal Chem 403, 1019–1029 (2012). https://doi.org/10.1007/s00216-011-5703-y

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  • DOI: https://doi.org/10.1007/s00216-011-5703-y

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