Abstract
Well-dispersed gold nanoparticles (NP) coated with tiopronin were synthesized by X-ray irradiation without reducing agents. High-resolution transmission electron microscopy shows that the average core diameters of the NPs can be systematically controlled by adjusting the tiopronin to Au mole ratio in the reaction. Three methods were used to study the NP uptake by cells: quantitative measurements by inductively coupled plasma mass spectrometry, direct imaging with high lateral resolution transmission electron microscopy and transmission X-ray microscopy. The results confirmed that the NP internalization mostly occurred via endocytosis and concerned the cytoplasm. The particles, in spite of their small sizes, were not found to arrive inside the cell nuclei. The synthesis without reducing agents and solvents increased the biocompatibility as required for potential applications in analysis and biomedicine in general.
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References
Daniel MC, Astruc D (2003) Chem Rev 104:293–346
Dahl JA, Maddux BLS, Hutchison JE (2007) Chem Rev 107:2228–2269
Funston AM, Mulvaney P, Murray RW (2009) Langmuir 25:13840–13851
Brust M, Fink J, Bethell D, Schiffrin DJ, Kiely CJ (1995) Chem Commun 16:1655–1656
Brust M, Schiffrin DJ, Bethell D, Kiely CJ (1995) Adv Mater 7:795–797
Brown LO, Hutchison JE (1997) J Am Chem Soc 119:12384–12385
Chen S, Murray RW (1998) Langmuir 15:682–689
Templeton AC, Chen S, Gross SM, Murray RW (1998) Langmuir 15:66–76
Schaaff TG, Whetten RL (2000) J Phys Chem B 104:2630–2641
Pasquato L, Pengo P, Scrimin P (2004) J Mater Chem 14:3481–3487
Price RC, Whetten RL (2005) J Am Chem Soc 127:13750–13751
Bertino MF, Sun ZM, Zhang R, Wang LS (2006) J Phys Chem B 110:21416–21418
Jin R (2008) Agew Chem Int Ed 47:6750–6753
Wu Z, Suhan J, Jin R (2009) J Mater Chem 19:622–626
Zhu Y, Qian H, Zhu M, Jin R (2010) Adv Mater 22:1915–1920
Tang Z, Xu B, Wu B, Germann MW (2010) J Am Chem Soc 132:3367–3374
Heaven MW, Dass A, White PS, Holt KM, Murray RW (2008) J Am Chem Soc 130:3754–3755
Jadzinsky PD, Calero G, Ackerson CJ, Bushnell DA, Kornberg RD (2007) Science 318:430–433
Seino S, Kinoshita T, Nakagawa T, Kojima T, Taniguci R, Okuda S, Yamamoto T (2008) J Nanopart Res 10:1071–1076
Ma Q, Moldovan N, Mancini DC, Rosenberg RA (2000) Appl Phys Lett 76:2014–2016
Karadas F, Ertas G, Ozkaraoglu E, Suzer S (2004) Langmuir 21:437–442
Cai XQ, Wang CL, Chen HH, Chie CC, Lai SF, Chen YY, Hua TE, Kempson IM, Hwu Y, Yang CS, Margaritondo G (2010) Nanotechnology 21:335604
Wang CH, Liu CR, Wang CL, Hua TE, Lee KH, Hwu YK (2008) J Phys D Appl Phys 41:195301–195308
Wang CH, Hua TE, Chien CC, Yu YL, Yang TY, Liu CJ, Leng WH, Hwu YK, Yang YC, Kim CC, Je JH, Chen CH, Lin HM, Margaritondo G (2007) Mater Chem Phys 106:323–329
Wang CL, Hso BJ, Lai SF, Chen WC, Chen HH, Chen YY, Chien CC, Cai XQ, Kempson IM, Hwu Y, Margaritondo G (2011) Nanotechnology 22:065605
Alkilany AM, Nagaria PK, Hexel CR, Shaw TJ, Murphy CJ, Wyatt MD (2009) Small 5:701–708
Chithrani BD, Ghazani AA, Chan WCW (2006) Nano Lett 6:662–668
Chithrani BD, Chan WCW (2007) Nano Lett 7:1542–1550
Nativo P, Prior IA, Brust M (2008) ACS Nano 2:1639–1644
Chu YS, Yi JM, De Carlo F, Shen Q, Lee WK, Wu HJ, Wang CL, Wang JY, Liu CJ, Wang CH, Wu SR, Chien CC, Hwu YK, Tkachuk A, Yun W, Feser M, Liang KS, Je JH, Margaritondo G (2008) Appl Phys Lett 92:103119–103123
Chen YT, Chen TY, Yi J, Chu YS, Lee WK, Wang CL, Kempson IM, Hwu Y, Gajdosik V, Margaritondo G (2011) Opt Lett 36:1269–1271
Syme CD, Sirimuthu NM, Faley SL, Cooper JM (2010) Chem Commun 46:7921–7923
Sirimuthu NMS, Syme CD, Cooper JM (2010) Anal Chem 82:7369–7373
Le Gros MA, McDermott G, Larabell CA (2005) Curr Opin Struck Biol 15:593–600
Larabell CA, Le Gros MA (2004) Mol Biol Cell 15:957–962
Hsu PC, Wang CH, Yang TY, Hwu YK, Lin CS, Chen CH, Chang LW, Seol SK, Je JH, Margaritondo G (2007) J Vac Sci Technol A 25:615–620
Kim YG, Oh SK, Crooks RM (2003) Chem Mater 16:167–172
Liu CJ, Wang CH, Chen ST, Chen HH, Leng WH, Chien CC, Wang CL, Kempson IM, Hwu Y, Lai TC, Hsiao M, Yang CS, Chen YJ, Margaritondo G (2010) Phys Med Biol 55:930–945
Aft RL, Lewis JS, Zhang F, Kim J, Welch MJ (2003) Cancer Res 63:5496–5504
Huang FK, Chen WC, Lai SF, Liu CJ, Wang CL, Wang CH, Chen HH, Hua TE, Chen YY, Wu MK, Hwu Y, Yang CS, Margaritondo G (2010) Phys Med Biol 55:469–482
Chen HH, Chien CC, Petibois C, Wang CL, Chu YS, Lai SF, Hua TE, Chen YY, Cai XQ, Hwu Y, Margaritondo G (2011) J Nanobiotechnology 9(1):14
Wang CH, Liu CJ, Chien CC, Chen HT, Hua TE, Leng WH, Chen HH, Kempson IM, Hwu Y, Hsiao M, Lai TC, Wang JL, Yang CS, Lin HM, Chen YJ, Margaritondo G (2011) Mater Chem Phys 126:352–356
Wörle-Knirsch JM, Pulskamp K, Krug HF (2006) Nano Lett 6:1261–1268
Yen HJ, Hsu SH, Tsai CL (2009) Small 5:1553–1561
Castaneda L, Valle J, Yang N, Pluskat S, Slowinska K (2008) Biomacromolecules 9:3383–3388
De La Fuente JM, Berry CC, Riehle MO, Curtis AS (2006) Langmuir 22:3286–3293
Mobius W, van Donselaar E, Ohno-Iwashita Y, Shimada Y, Heijnen HF, Slot JW, Geuze HJ (2003) Traffic 4:222–231
Pelkmans L, Burli T, Zerial M, Helenius A (2004) Cell 118:767–780
Tkachenko AG, Xie H, Liu Y, Coleman D, Ryan J, Glomm WR, Shipton MK, Franzen S, Feldheim DL (2004) Bioconjug Chem 15:482–490
Acknowledgments
This research was supported by the National Science and Technology Program for Nanoscience and Nanotechnology, the Thematic Research Project of Academia Sinica, the Biomedical Nano-Imaging Core Facility at National Synchrotron Radiation Research Center (Taiwan), the Fonds National Suisse pour la Recherche Scientifique, and by the Center for Biomedical Imaging (CIBM, supported by the Louis-Jeantet and Leenards foundations). The Advanced Photon Source was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences, under the Contract No. DE-AC02-06CH11357.
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Published in the special issue Imaging Techniques with Synchrotron Radiation with Guest Editor Cyril Petibois.
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Cai, X., Chen, HH., Wang, CL. et al. Imaging the cellular uptake of tiopronin-modified gold nanoparticles. Anal Bioanal Chem 401, 809–816 (2011). https://doi.org/10.1007/s00216-011-4986-3
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DOI: https://doi.org/10.1007/s00216-011-4986-3