Skip to main content
SpringerLink
Log in

Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for proteome research

  • Review
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

High-performance liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC–ESI-MS–MS) is regarded as one of the most powerful techniques for separation and identification of proteins. Recently, much effort has been made to improve the separation capacity, detection sensitivity, and analysis throughput of micro- and nano-HPLC, by increasing column length, reducing column internal diameter, and using integrated techniques. Development of HPLC columns has also been rapid, as a result of the use of submicrometer packing materials and monolithic columns. All these innovations result in clearly improved performance of micro- and nano-HPLC for proteome research.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Fournier ML, Gilmore JM, Martin-Brown SA, Washburn MP (2007) Chem Rev 107:3654–3686

    Article  CAS  Google Scholar 

  2. Wickware P, Smaglik P (2001) Nature 413:869–875

    Article  CAS  Google Scholar 

  3. O'Farrell PH (1975) J Biol Chem 250:4007–4021

    Google Scholar 

  4. Klose J (1975) Humangenetik 26:231–243

    CAS  Google Scholar 

  5. Motoyama A, Yates JR 3rd (2008) Anal Chem 80:7187–7193

    Article  CAS  Google Scholar 

  6. Yates JR, Ruse CI, Nakorchevsky A (2009) Annu Rev Biomed Eng 11:49–79

    Article  CAS  Google Scholar 

  7. Sprung RW Jr, Brock JW, Tanksley JP, Li M, Washington MK, Slebos RJ, Liebler DC (2009) Mol Cell Proteomics 8:1988–1998

    Article  CAS  Google Scholar 

  8. Yuan H, Zhou Y, Zhang L, Liang Z, Zhang Y (2009) J Chromatogr A 1216:7478–7482

    Article  CAS  Google Scholar 

  9. Kubota K, Kosaka T, Ichikawa K (2009) Methods Mol Biol 519:483–494

    Article  CAS  Google Scholar 

  10. Jiang X, Feng S, Tian R, Han G, Jiang X, Ye M, Zou H (2007) Proteomics 7:528–539

    Article  CAS  Google Scholar 

  11. Giddings JC (1984) Anal Chem 56:1258A–1260A

    Article  CAS  Google Scholar 

  12. MacNair JE, Lewis KC, Jorgenson JW (1997) Anal Chem 69:983–989

    Article  CAS  Google Scholar 

  13. Winter D, Seidler J, Ziv Y, Shiloh Y, Lehmann WD (2009) J Proteome Res 8:418–424

    Article  CAS  Google Scholar 

  14. Bordeerat NK, Georgieva NI, Klapper DG, Collins LB, Cross TJ, Borchers CH, Swenberg JA, Boysen G (2009) Proteomics 9:3939–3944

    Article  CAS  Google Scholar 

  15. Shen Y, Zhao R, Berger SJ, Anderson GA, Rodriguez N, Smith RD (2002) Anal Chem 74:4235–4249

    Article  CAS  Google Scholar 

  16. Min HK, Hyung SW, Shin JW, Nam HS, Ahn SH, Jung HJ, Lee SW (2007) Electrophoresis 28:1012–1021

    Article  CAS  Google Scholar 

  17. Tao D, Zhu G, Sun L, Ma J, Liang Z, Zhang W, Zhang L, Zhang Y (2009) Proteomics 9:2029–2036

    Article  CAS  Google Scholar 

  18. Shen Y, Smith RD, Unger KK, Juma D, Lubda F (2005) Anal Chem 77:6692–6701

    Article  CAS  Google Scholar 

  19. Guryca V, Kieffer-Jaquinod S, Garin J, Masselon CD (2008) Anal Bioanal Chem 392:1291–1297

    Article  CAS  Google Scholar 

  20. Dong M, Wu M, Wang F, Qin H, Han G, Dong J, Wu R, Ye M, Liu Z, Zou H (2010) Anal Chem 82:2907–2915

    Article  CAS  Google Scholar 

  21. van de Meent MH, de Jong GJ (2009) J Sep Sci 32:487–493

    Article  Google Scholar 

  22. Xie C, Ye M, Jiang X, Jin W, Zou H (2006) Mol Cell Proteomics 5:454–461

    CAS  Google Scholar 

  23. Guiochon G (2007) J Chromatogr A 1168:101–168

    Article  CAS  Google Scholar 

  24. Greiderer A, Trojer L, Huck CW, Bonn GK (2009) J Chromatogr A 1216:7747–7754

    Article  CAS  Google Scholar 

  25. Luo Q, Shen Y, Hixson KK, Zhao R, Yang F, Moore RJ, Mottaz HM, Smith RD (2005) Anal Chem 77:5028–5035

    Article  CAS  Google Scholar 

  26. Luo Q, Page JS, Tang K, Smith RD (2007) Anal Chem 79:540–545

    Article  CAS  Google Scholar 

  27. Jiang X, Dong J, Wang F, Feng S, Ye M, Zou H (2008) Electrophoresis 29:1612–1618

    Article  CAS  Google Scholar 

  28. Luo Q, Tang K, Yang F, Elias A, Shen Y, Moore RJ, Zhao R, Hixson KK, Rossie SS, Smith RD (2006) J Proteome Res 5:1091–1097

    Article  CAS  Google Scholar 

  29. Yue G, Luo Q, Zhang J, Wu SL, Karger BL (2007) Anal Chem 79:938–946

    Article  CAS  Google Scholar 

  30. Luo Q, Rejtar T, Wu SL, Karger BL (2009) J Chromatogr A 1216:1223–1231

    Article  CAS  Google Scholar 

  31. Zhang J, Wu SL, Kim J, Karger BL (2007) J Chromatogr A 1154:295–307

    Article  CAS  Google Scholar 

  32. Robotti KM, Yin H, Brennen R, Trojer L, Killeen K (2009) J Sep Sci 32:3379–3387

    Article  CAS  Google Scholar 

  33. Schlund M, Gilbert SE, Schnydrig S, Renaud P (2007) Sens Actuators B 123:1133–1141

    Article  Google Scholar 

  34. Ehlert S, Kraiczek K, Mora JA, Dittmann M, Rozing GP, Tallarek U (2008) Anal Chem 80:5945–5950

    Article  CAS  Google Scholar 

  35. Jung S, Ehlert S, Mora JA, Kraiczek K, Dittmann M, Rozing GP, Tallarek U (2009) J Chromatogr A 1216:264–273

    Article  CAS  Google Scholar 

  36. De Malsche W, Clicq D, Verdoold V, Gzil P, Desmet G, Gardeniers H (2007) Lab Chip 7:1705–1711

    Article  Google Scholar 

  37. De Malsche W, Eghbali H, Clicq D, Vangelooven J, Gardeniers H, Desmet G (2007) Anal Chem 79:5915–5926

    Article  Google Scholar 

  38. Levkin PA, Eeltink S, Stratton TR, Brennen R, Robotti K, Yin H, Killeen K, Svec F, Fréchet JM (2008) J Chromatogr A 1200:55–61

    Article  CAS  Google Scholar 

  39. Yin H, Killeen K, Brennen R, Sobek D, Werlich M, van de Goor T (2005) Anal Chem 77:527–533

    Article  CAS  Google Scholar 

  40. Chu CS, Niñonuevo MR, Clowers BH, Perkins PD, An HJ, Yin H, Killeen K, Miyamoto S, Grimm R, Lebrilla CB (2009) Proteomics 9:1939–1951

    Article  CAS  Google Scholar 

  41. Ellis J, Grimm R, Clark JF, Pyne-Gaithman G, Wilbur S, Caruso JA (2008) J Proteome Res 7:4736–4742

    Article  CAS  Google Scholar 

  42. Callipo L, Foglia P, Gubbiotti R, Samperi R, Laganà A (2009) Anal Bioanal Chem 394:811–820

    Article  CAS  Google Scholar 

  43. Brambilla F, Resta D, Isak I, Zanotti M, Arnoldi A (2009) Proteomics 9:272–286

    Article  CAS  Google Scholar 

  44. Groleau PE, Desharnais P, Coté L, Ayotte C (2008) J Mass Spectrom 43:924–935

    Article  CAS  Google Scholar 

  45. Alley WR Jr, Mechref Y, Novotny MV (2009) Rapid Commun. Mass Spectrom 23:495–505

    CAS  Google Scholar 

  46. Raijmakers R, Kraiczek K, de Jong AP, Mohammed S, Heck AJ (2010) Anal Chem 82:824–832

    Article  CAS  Google Scholar 

  47. Staples GO, Bowman MJ, Costello CE, Hitchcock AM, Lau JM, Leymarie N, Miller C, Naimy H, Shi X, Zaia J (2009) Proteomics 9:686–695

    Article  CAS  Google Scholar 

  48. Staples GO, Naimy H, Yin H, Kileen K, Kraiczek K, Costello CE, Zaia J (2010) Anal Chem 82:516–522

    Article  CAS  Google Scholar 

  49. Liu J, Chen CF, Tsao CW, Chang CC, Chu CC, DeVoe DL (2009) Anal Chem 81:2545–2554

    Article  CAS  Google Scholar 

  50. Kulasingam V, Diamandis EP (2007) Mol Cell Proteomics 6:1997–2011

    Article  CAS  Google Scholar 

  51. Gao M, Deng C, Yu W, Zhang Y, Yang P, Zhang X (2008) Proteomics 8:939–947

    Article  CAS  Google Scholar 

  52. Wang N, Xie C, Young JB, Li L (2009) Anal Chem 81:1049–1060

    Article  CAS  Google Scholar 

  53. Cho CK, Shan SJ, Winsor EJ, Diamandis EP (2007) Mol Cell Proteomics 6:1406–1415

    Article  CAS  Google Scholar 

  54. Han G, Ye M, Zhou H, Jiang X, Feng S, Jiang X, Tian R, Wan D, Zou H, Gu J (2008) Proteomics 8:1346–1361

    Article  CAS  Google Scholar 

  55. Delmotte N, Lasaosa M, Tholey A, Heinzle E, Huber CG (2007) J Proteome Res 6:4363–4373

    Article  CAS  Google Scholar 

  56. Nakamura T, Kuromitsu J, Oda Y (2008) J Proteome Res 7:1007–1011

    Article  CAS  Google Scholar 

  57. Lasaosa M, Delmotte N, Huber CG, Melchior K, Heinzle E, Tholey A (2009) Anal Bioanal Chem 393:1245–1256

    Article  CAS  Google Scholar 

  58. Lasaosa M, Jakoby T, Delmotte N, Huber CG, Heinzle E, Tholey A (2009) Proteomics 9:4577–4581

    Article  CAS  Google Scholar 

  59. Wörner M, Melchior K, Delmotte N, Hwang KH, Monostory K, Huber CG, Bernhardt R (2009) J Sep Sci 32:1165–1174

    Article  Google Scholar 

  60. Song C, Ye M, Han G, Jiang X, Wang F, Yu Z, Chen R, Zou H (2009) Anal Chem 82:53–56

    Article  Google Scholar 

  61. Zhu F, Kale AV, Cheryan M (2007) J Agric Food Chem 55:3843–3849

    Article  CAS  Google Scholar 

  62. Zhang J, Xu X, Gao M, Yang P, Zhang X (2007) Proteomics 7:500–512

    Article  CAS  Google Scholar 

  63. Boersema PJ, Divecha N, Heck AJ, Mohammed S (2007) J Proteome Res 6:937–946

    Article  CAS  Google Scholar 

  64. Link AJ, Eng J, Schieltz DM, Carmack E, Mize GJ, Morris DR, Garvik BM, Yates JR 3rd (1999) Nat Biotechnol 17:676–682

    Article  CAS  Google Scholar 

  65. Washburn MP, Wolters D, Yates JR 3rd (2001) Nat Biotechnol 19:242–247

    Article  CAS  Google Scholar 

  66. Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR 3rd (2009) J Proteome Res 8:1304–1314

    Article  CAS  Google Scholar 

  67. Dai J, Shieh CH, Sheng QH, Zhou H, Zeng R (2005) Anal Chem 77:5793–5799

    Article  CAS  Google Scholar 

  68. Motoyama A, Xu T, Ruse CI, Wohlschlegel JA, Yates JR 3rd (2007) Anal Chem 79:3623–3634

    Article  CAS  Google Scholar 

  69. Wang F, Dong J, Ye M, Wu R, Zou H (2009) J Chromatogr A 1216:3887–3894

    Article  CAS  Google Scholar 

  70. Andrews GL, Shuford CM, Burnett JC Jr, Hawkridge AM, Muddiman DC (2009) J Chromatogr B 877:948–954

    Article  CAS  Google Scholar 

  71. Guzzetta AW, Chien AS (2005) J Proteome Res 4:2412–2419

    Article  CAS  Google Scholar 

  72. Licklider LJ, Thoreen CC, Peng J, Gygi SP (2002) Anal Chem 74:3076–3083

    Article  CAS  Google Scholar 

  73. Wang F, Dong J, Jiang X, Ye M, Zou H (2007) Anal Chem 79:6599–6606

    Article  CAS  Google Scholar 

  74. Luo Q, Yue G, Valaskovic GA, Gu Y, Wu SL, Karger BL (2007) Anal Chem 79:6174–6181

    Article  CAS  Google Scholar 

  75. Luo Q, Gu Y, Wu SL, Rejtar T, Karger BL (2008) Electrophoresis 29:1604–1611

    Article  CAS  Google Scholar 

  76. Shen Y, Moore RJ, Zhao R, Blonder J, Auberry DL, Masselon C, Pasa-Tolić L, Hixson KK, Auberry KJ, Smith RD (2003) Anal Chem 75:3596–3605

    Article  CAS  Google Scholar 

  77. Zhou H, Dai J, Sheng QH, Li RX, Shieh CH, Guttman A, Zeng R (2007) Electrophoresis 28:4311–4319

    Article  CAS  Google Scholar 

  78. Mihailova A, Malerød H, Wilson SR, Karaszewski B, Hauser R, Lundanes E, Greibrokk T (2008) J Sep Sci 31:459–467

    Article  CAS  Google Scholar 

  79. Bedani F, Kok WT, Janssen HG (2006) J Chromatogr A 1133:126–134

    Article  CAS  Google Scholar 

  80. Lin S, Yun D, Qi D, Deng C, Li Y, Zhang X (2008) J Proteome Res 7:1297–1307

    Article  CAS  Google Scholar 

  81. Cingöz A, Hugon-Chapuis F, Pichon V (2008) J Chromatogr A 1209:95–103

    Article  Google Scholar 

  82. Liu Y, Lu H, Zhong W, Song P, Kong J, Yang P, Girault HH, Liu B (2006) Anal Chem 78:801–808

    Article  CAS  Google Scholar 

  83. Li Y, Yan B, Deng C, Tang J, Liu J, Zhang X (2007) Proteomics 7:3661–3671

    Article  CAS  Google Scholar 

  84. Stigter EC, de Jong GJ, van Bennekom WP (2008) Anal Chim Acta 619:231–238

    Article  CAS  Google Scholar 

  85. Gao M, Zhang P, Hong G, Guan X, Yan G, Deng C, Zhang X (2009) J Chromatogr A 1216:7472–7477

    Article  CAS  Google Scholar 

  86. Duan J, Liang Z, Yang C, Zhang J, Zhang L, Zhang W, Zhang Y (2006) Proteomics 6:412–419

    Article  CAS  Google Scholar 

  87. Feng S, Ye M, Jiang X, Jin W, Zou H (2006) J Proteome Res 5:422–428

    Article  CAS  Google Scholar 

  88. Krenkova J, Lacher NA, Svec F (2009) J Chromatogr A 1216:3252–3259

    Article  CAS  Google Scholar 

  89. Ma J, Liu J, Sun L, Gao L, Liang Z, Zhang L, Zhang Y (2009) Anal Chem 81:6534–6540

    Article  CAS  Google Scholar 

  90. Yuan H, Zhang L, Hou C, Zhu G, Tao D, Liang Z, Zhang Y (2009) Anal Chem 81:8708–8714

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors are grateful for financial support from the National Basic Research Program of China (2007CB914100), the National Natural Science Foundation (20935004), the Knowledge Innovation Program of Chinese Academy of Sciences (KJCX2YW.H09), and DFG-NSFC cooperation project (GZ3164).

Author information

Authors and Affiliations

  1. Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Dingyin Tao, Lihua Zhang, Yichu Shan, Zhen Liang & Yukui Zhang

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100039, China

    Dingyin Tao

  3. National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China

    Lihua Zhang

Authors
  1. Dingyin Tao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lihua Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yichu Shan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhen Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yukui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lihua Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tao, D., Zhang, L., Shan, Y. et al. Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for proteome research. Anal Bioanal Chem 399, 229–241 (2011). https://doi.org/10.1007/s00216-010-3946-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-010-3946-7

Keywords

Search

Navigation