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Certification of drugs of abuse in a human serum standard reference material: SRM 1959

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Abstract

A new standard reference material (SRM) for drugs of abuse in human serum (SRM 1959) has been developed. This SRM is intended to be used as a control material for laboratories performing analysis of drugs of abuse in blood to evaluate the accuracy of their methods. SRM 1959 is a frozen human serum material fortified with seven compounds for which analyses are performed to determine evidence of illegal drug use: benzoylecgonine (BZE), methadone (METH), methamphetamine (MAMP), morphine (MOR), nordiazepam (NOR), phencyclidine (PCP), and 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-9-COOH). Two independent methods involving isotope dilution (ID)-gas chromatography/mass spectrometry (GC/MS) and ID-liquid chromatography/mass spectrometry (LC/MS) were used for the value assignment. For THC-9-COOH, an additional measurement using LC/tandem mass spectrometry (LC/MS/MS) was also included. All methods used isotopically labeled compounds as internal standards and solid-phase extractions to isolate the analytes from the serum. The GC/MS methods used different clean-up procedures from those used for the LC/MS-based methods. Repeatability with within-set coefficients of variation (CVs) ranged from 0.5% to 4.3% for the GC/MS methods and from 0.2% to 1.2% for the LC/MS-based methods. Intermediate precision with between-set CVs for all the methods ranged from 0.1% to 1.1%. Agreement between the GC/MS and LC/MS methods ranged from 0.8% to 8.8%. The results from the methods were combined to obtain the certified concentrations and their expanded uncertainties.

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Acknowledgments

We thank Michele Schantz (NIST) for GC-FID analysis, and Tom Vetter and Brian Long (NIST) for Karl Fischer analysis for the purity assessment of the reference compounds. We thank Lane Sander, Stephen Wise, and Diane Hancock, all from NIST, for reviewing the manuscript.

Disclaimer

Certain commercial equipment, instruments, and materials are identified in this paper to adequately specify the experimental procedure. Such identification does not imply recommendation or endorsement by NIST nor does it imply that the equipment, instruments, or materials are necessarily the best available for the purpose.

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Authors and Affiliations

  1. Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA

    Susan S.-C. Tai, Jocelyn L. Prendergast, Lorna T. Sniegoski, Michael J. Welch & Karen W. Phinney

  2. Statistical Engineering Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA

    Nien Fan Zhang

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  1. Susan S.-C. Tai
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  2. Jocelyn L. Prendergast
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  3. Lorna T. Sniegoski
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  4. Michael J. Welch
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  5. Karen W. Phinney
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  6. Nien Fan Zhang
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Corresponding author

Correspondence to Susan S.-C. Tai.

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Tai, S.SC., Prendergast, J.L., Sniegoski, L.T. et al. Certification of drugs of abuse in a human serum standard reference material: SRM 1959. Anal Bioanal Chem 397, 501–509 (2010). https://doi.org/10.1007/s00216-010-3603-1

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  • DOI: https://doi.org/10.1007/s00216-010-3603-1

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