Skip to main content
SpringerLink
Log in

Identification of a lipoarabinomannan-like lipoglycan in the actinomycete Gordonia bronchialis

  • Short Communication
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

The cell envelopes of actinomycetes contain lipidated macroamphiphiles, of which the most extensively characterised are the lipoarabinomannans of mycobacteria and related bacteria. We have investigated the mycolic acid-containing actinomycete Gordonia bronchialis and identified the presence of a lipoarabinomannan-like lipoglycan. The extraction and purification procedures recovered a second amphiphilic fraction with properties suggesting a phosphatidylinositol mannoside, consistent with studies of other Gordonia species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Arenskötter M, Bröker D, Steinbüchel A (2004) Biology of the metabolically diverse genus Gordonia. Appl Environm Microbiol 70:3195–3204

    Article  CAS  Google Scholar 

  • Fischer W (1994) Lipoteichoic acids and lipoglycans. In: Ghuysen J-M, Hakenbeck R (eds) New comprehensive biochemistry: bacterial cell wall. Vol 27. Elsevier, Amsterdam, pp 199–215

  • Flaherty C, Sutcliffe IC (1999) Identification of a lipoarabinomannan-like lipoglycan in Gordonia rubropertincta. Syst Appl Microbiol 22:530–533

    PubMed  CAS  Google Scholar 

  • Furneaux RH, Landersjö CL, McCullough JL, Severn WB (2005) A novel phosphatidylinositol manno-oligosaccharide (dPIM-8) from Gordonia sputi. Carbohydr Res 340:1618–1624

    Article  PubMed  CAS  Google Scholar 

  • Garton NJ, Gilleron M, Brando T, Dan HH, Giguere S, Puzo G, Prescott JF, Sutcliffe IC (2002) A novel lipoarabinomannan from the equine pathogen Rhodococcus equi Structure and effect on macrophage cytokine production. J Biol Chem 277: 31722–31733

    Article  PubMed  CAS  Google Scholar 

  • Gilleron M, Garton NJ, Nigou J, Brando T, Puzo G, Sutcliffe IC (2005) Characterisation of a truncated lipoarabinomannan from the actinomycete Turicella otitidis. J Bacteriol 187:854–861

    Article  PubMed  CAS  Google Scholar 

  • Klatte S, Kroppenstedt RM, Schumann P, Altendorf K, Rainey FA (1996) Gordona hirsuta sp. nov. Int J Syst Bacteriol 46:876–880

    Article  PubMed  CAS  Google Scholar 

  • Nigou J, Gilleron M, Puzo G (2003) Lipoarabinomannans: from structure to biosynthesis. Biochimie 85:153–166

    Article  PubMed  CAS  Google Scholar 

  • Sutcliffe IC (1994) The lipoteichoic acids and lipoglycans of Gram-positive bacteria: a chemotaxonomic perspective. Syst Appl Microbiol 17:467-480

    Google Scholar 

  • Sutcliffe IC (2000) Characterisation of a lipomannan lipoglycan from the mycolic acid containing actinomycete Dietzia maris. Antonie Van Leeuwenhoek 78:195–201

    Article  PubMed  CAS  Google Scholar 

  • Sutcliffe IC (2005) Lipoarabinomannans—structurally diverse and functionally enigmatic macroamphiphiles of mycobacteria and related actinomycetes. Tuberculosis 85:205–206

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

we are grateful to Dr. John Belisle (Department of Microbiology, Colorado State University, USA) for supplying the polyclonal anti-LAM antibody (NIH sponsorship N01-AI-25147). Experimental work in this study was mainly carried out at the University of Sunderland, Institute of Pharmacy with the financial support of the Wellcome Trust (project 047462/Z/96) and Horserace Betting Levy Board (project:: vet/prj/652). We are grateful to the late Dr. David Bendell for assistance with the GC-MS experiments.

Author information

Authors and Affiliations

  1. Department of Infection, Immunity and Inflammation, Leicester University, Medical Sciences Building, University Road, PO Box 138, LE1 9HN, Leicester, UK

    Natalie J. Garton

  2. Division of Biomedical Sciences, School of Applied Sciences, Northumbria University, Newcastle upon, NE1 8ST, Tyne, UK

    Iain C. Sutcliffe

Authors
  1. Natalie J. Garton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Iain C. Sutcliffe
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Iain C. Sutcliffe.

Additional information

Dedicated to the memory of our former colleague Dr. David Bendell.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Garton, N.J., Sutcliffe, I.C. Identification of a lipoarabinomannan-like lipoglycan in the actinomycete Gordonia bronchialis . Arch Microbiol 184, 425–427 (2006). https://doi.org/10.1007/s00203-005-0050-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-005-0050-z

Keywords

Search

Navigation