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Nuclear translocation of the heterotrimeric CCAAT binding factor of Aspergillus oryzae is dependent on two redundant localising signals in a single subunit

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Abstract

The CCAAT-binding complex in the Aspergillus species, also known as the Hap complex, consists of at least three subunits, namely HapB, HapC and HapE. Each Hap subunit contains an evolutionary conserved core domain. Recently, we have found that the HapC and HapE subunits do not carry a nuclear localisation signal. Furthermore, when in complex with HapB, they are transported into the nucleus via a ‘piggy back mechanism’ in A. nidulans. To extend our findings to other filamentous fungi, we examined the nuclear localisation of the A. oryzae Hap subunits by analysing several GFP fusion proteins with these Hap subunits in the hap deletion strains of A. nidulans. The nuclear translocation of the A. oryzae complex was found to be dependent on two redundant localising signals in HapB.

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Acknowledgements

We thank Dr. Yasuo Niwa for the generous gift of a plasmid carrying a GFP gene.

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Author notes

  1. Stefan Steidl

    Present address: MorphoSys AG, Lena-Christ-Str. 48, 82152, Martinsried, Germany

  2. Norihiro Tsukagoshi

    Present address: The University of the Air, 101-2 Yagoto-honmachi, Nagoya, 466-0825, Japan

Authors and Affiliations

  1. Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan

    Hideya Goda, Takashi Nagase, Shinjiro Tanoue, Junya Sugiyama, Tetsuo Kobayashi, Norihiro Tsukagoshi & Masashi Kato

  2. Department of Molecular and Applied Microbiology, Leibniz-Institute for Natural Products Research and Infection Biology (HKI), Beutenbergstrasse 11a, D-07745, Jena, Germany

    Stefan Steidl, André Tüncher & Axel A. Brakhage

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  1. Hideya Goda
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  2. Takashi Nagase
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  9. Axel A. Brakhage
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Correspondence to Masashi Kato.

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Goda, H., Nagase, T., Tanoue, S. et al. Nuclear translocation of the heterotrimeric CCAAT binding factor of Aspergillus oryzae is dependent on two redundant localising signals in a single subunit. Arch Microbiol 184, 93–100 (2005). https://doi.org/10.1007/s00203-005-0014-3

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  • DOI: https://doi.org/10.1007/s00203-005-0014-3

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