Abstract
Adult mortality, biomass, fecundity and viability of cocoons were studied in Eisenia fetida and Octolasion tyrtaeum, in response to glyphosate exposure in soil. Exposure tests were carried out following USEPA procedure, with five concentrations of glyphosate in soil and a control. O. tyrtaeum was more sensitive to the highest concentration of glyphosate (50,000 mg kg−1), with 100 % mortality by day 7 of exposure, compared with 71 % for E. fetida. Although biomass of O. tyrtaeum was significantly different between the control and 5,000 mg kg−1 dose at day 14, E. fetida was not affected at that concentration, and only showed a significant weight loss after 7 days of exposure to 50,000 mg kg−1. Adverse effects upon adult fecundity and cocoon viability were observed at glyphosate concentrations of 5,000 mg kg−1 and above. Adverse effects were observed at concentrations that greatly exceeded the recommended field application rates of glyphosate.
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References
Aparicio VC, De Gerónimo E, Marino D, Primost J, Carriquiriborde P, Costa JL (2013) Environmental fate of glyphosate and aminomethylphosphonic acid in surface waters and soil of agricultural basins. Chemosphere 93(9):1866–1873
Bierkens J, Klein G, Corbisier P, Van Den Heuvel R, Verschaeve L (1998) Comparative sensitivity of 20 bioassays for soil quality. Chemosphere 37(14–15):2935–2947
Casabé N, Piola L, Fuchs J, Oneto ML, Pamparato L, Basack S, Giménez R, Massaro R, Papa JC, Kesten E (2007) Ecotoxicological assessment of the effects of glyphosate and chlorpyrifos in an Argentine soya field. J Soils Sediments 7(4):232–239
Conover WJ, Iman RL (1981) Rank transformations as a bridge between parametric and nonparametric statistics. Am Stat 35:124–129
Correia FV, Moreira JC (2010) Effects of glyphosate and 2,4-D on earthworms (Eisenia fetida) in laboratory tests. Bull Environ Contam Toxicol 85:264–268
Ibañez M, Pozo OJ, Sancho JV, López FJ, Hernández F (2005) Residue determination of glyphosate, glufosinate and aminomethylphosphonic acid in water and soil samples by liquid chromatography coupled to electrospray tandem mass spectrometry. J Chromatogr A 1081:145–155
ISO International Organization for Standardization (1998) Soil quality: effects of pollutants on earthworms (Eisenia fetida). Part 2. Determination of effects on reproduction. ISO 11268-2. Geneva, Switzerland
Khoury GA, Gehris TC, Tribe L, Torres Sánchez RM, Afonso MS (2010) Glyphosate adsorption on montmorillonite: an experimental and theoretical study of surface complexes. Appl Clay Sci 50(2):167–175
Kolpin DW, Thurman EM, Lee EA, Meyer MT, Furlong ET, Glassmeyer ST (2006) Urban contributions of glyphosate and its degradate AMPA to streams in the United States. Sci Total Environ 354:191–197
Marques CR, Pereira R, Gonçalves F (2009) Using earthworm avoidance behaviour to assess the toxicity of formulated herbicides and their active ingredients on natural soils. J Soils Sediments 9:137–147
Marques CR, Gonçalves AMM, Pereira R, Gonçalves F (2012) Ecotoxicological effects of MIKADO and VIPER on algae and daphnids. Environ Toxicol 2(12):685–699
Mischis CC (2000) Las lombrices de tierra (Annelida, Oligochaeta) de la República Argentina. Nat Neotrop 31:17–27
Mischis CC, Herrera JAD (2000) Lombrices de tierra: métodos para el estudio e identificación. Rev Educ Biol 3:36–43
Mischis CC, Righi G (1999) Contribution to knowledge of the Oligochaete fauna (Annelida, Oligochaeta) from Argentina. Gayana 63(2):11–13
PAN Pesticides Action Network (2010) Environmental effects of pesticides: an impression of recent scientific literature. www.pan-europe.info
Pereira JL, Antunes SC, Castro BB, Marques CR, Gonçalves AMM, Gonçalves F, Pereira R (2009) Toxicity evaluation of three pesticides in non aquatic and soil non target organisms: commercial formulation vs active ingredient. Ecotoxicology 18:455–463
Pessagno RC, Torres Sánchez RM, María dos Santos A (2008) Glyphosate behavior at soil and mineral-water interfaces. Environ Pollut 153(1):53–59
Shahla Y, D’Souza D (2010) Effects of pesticides on the growth and reproduction of earthworm: a review. Appl Environ Soil Sci 2010:1–10
USEPA (United States Environmental Protection Agency) (1993) Office of pesticide programs. Special Review and Reregistration Division. Reregistration eligibility decision (RED): Glyphosate. Washington, DC
USEPA (United States Environmental Protection Agency) (1996) Earthworm subchronic toxicity test. Ecological effects test guidelines. Prevention, pesticides and toxic substances OPPTS 850.6200. EPA 712–C–96–167
USEPA (United States Environmental Protection Agency) (2011) Benchmark dose software (BMDS). http://www.epa.gov/NCEA/bmds.htm
WHO (World Health Organization) (1994) Glyphosate. Environmental health criteria No. 159. United Nations Environment Programme, International Labour Organization. Ginebra, Suiza
Yasmin S, D’Souza D (2007) Effect of pesticides on the reproductive output of Eisenia fetida. Bull Environ Contam Toxicol 79:529–532
Yasmin S, D’Souza D (2010) Effects of pesticides on the growth and reproduction of earthworm: a Review. Appl Environ Soil Sci 10:1–9
Acknowledgments
This study was funded by ANPCyT and MAGyP (PID 0032/2011). The authors wish to thank Dr. Jorge Zavala for his useful comments; and Veronica Feuring and Alina Crelier for technical support.
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García-Torres, T., Giuffré, L., Romaniuk, R. et al. Exposure Assessment to Glyphosate of Two Species of Annelids. Bull Environ Contam Toxicol 93, 209–214 (2014). https://doi.org/10.1007/s00128-014-1312-8
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DOI: https://doi.org/10.1007/s00128-014-1312-8