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Mapping and characterization of seed dormancy QTLs using chromosome segment substitution lines in rice

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Abstract

Seed dormancy—the temporary failure of a viable seed to germinate under favorable conditions—is a complex characteristic influenced by many genes and environmental factors. To detect the genetic factors associated with seed dormancy in rice, we conducted a QTL analysis using chromosome segment substitution lines (CSSLs) derived from a cross between Nona Bokra (strong dormancy) and Koshihikari (weak dormancy). Comparison of the levels of seed dormancy of the CSSLs and their recurrent parent Koshihikari revealed that two chromosomal regions—on the short arms of chromosomes 1 and 6—were involved in the variation in seed dormancy. Further genetic analyses using an F2 population derived from crosses between the CSSLs and Koshihikari confirmed the allelic differences and the chromosomal locations of three putative QTLs: Sdr6 on chromosome 1 and Sdr9 and Sdr10 on chromosome 6. The Nona Bokra alleles of the three QTLs were associated with decreased germination rate. We discuss the physiological features of the CSSLs and speculate on the possible mechanisms of dormancy in light of the newly detected QTLs.

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Acknowledgments

We thank the Technical Support Section of NIAS for managing the rice field, and we thank T. Ando and I. Kono of the STAFF Institute for genotyping. We also thank the Rice Genome Resource Center (http://www.rgrc.dna.affrc.go.jp) for providing the CSSL seeds. This work was supported by grants from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Integrated Research Project for Plant, Insect, and Animal using Genome Technology QT-1005, Genomics for Agricultural Innovation NVR-0001, and Genomics for Agricultural Innovation IPG0010).

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Authors and Affiliations

  1. University of Tsukuba, 1-1-1, Tennohdai, Tsukuba, 305-8577, Japan

    Salem Marzougui & Masahiro Yano

  2. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602, Japan

    Salem Marzougui, Kazuhiko Sugimoto, Utako Yamanouchi, Masaki Shimono, Tomoki Hoshino, Kiyosumi Hori & Masahiro Yano

  3. BioResources Center, RIKEN, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan

    Masatomo Kobayashi & Kanako Ishiyama

Authors
  1. Salem Marzougui
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  2. Kazuhiko Sugimoto
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  3. Utako Yamanouchi
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  4. Masaki Shimono
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  5. Tomoki Hoshino
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  6. Kiyosumi Hori
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  7. Masatomo Kobayashi
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  8. Kanako Ishiyama
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  9. Masahiro Yano
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Corresponding author

Correspondence to Kazuhiko Sugimoto.

Additional information

Communicated by Y. Xu.

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Marzougui, S., Sugimoto, K., Yamanouchi, U. et al. Mapping and characterization of seed dormancy QTLs using chromosome segment substitution lines in rice. Theor Appl Genet 124, 893–902 (2012). https://doi.org/10.1007/s00122-011-1753-y

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  • DOI: https://doi.org/10.1007/s00122-011-1753-y

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