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Characterisation of a new stripe rust resistance gene Yr47 and its genetic association with the leaf rust resistance gene Lr52

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Abstract

Two Iranian common wheat landraces AUS28183 and AUS28187 from the Watkins collection showed high levels of seedling resistance against Australian pathotypes of leaf rust and stripe rust pathogens. Chi-squared analyses of rust response segregation among F3 populations derived from crosses of AUS28183 and AUS28187 with a susceptible genotype AUS27229 revealed monogenic inheritance of leaf rust and stripe rust resistance. As both genotypes produced similar leaf rust and stripe rust infection types, they were assumed to carry the same genes. The genes were temporarily named as LrW1 and YrW1. Molecular mapping placed LrW1 and YrW1 in the short arm of chromosome 5B, about 10 and 15 cM proximal to the SSR marker gwm234, respectively, and the marker cfb309 mapped 8–12 cM proximal to YrW1. LrW1 mapped 3–6 cM distal to YrW1 in two F3 populations. AUS28183 corresponded to the accession V336 of the Watkins collection which was the original source of Lr52. Based on the genomic location and accession records, LrW1 was concluded to be Lr52. Because no other seedling stripe rust resistance gene has previously been mapped in chromosome 5BS, YrW1 was permanently named as Yr47. A combination of flanking markers gwm234 and cfb309 with phenotypic assays could be used to ascertain the presence of Lr52 and Yr47 in segregating populations. This investigation characterised a valuable source of dual leaf rust and stripe rust resistance for deployment in new wheat cultivars. Transfer of Lr52 and Yr47 into current Australian wheat backgrounds is in progress.

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References

  • Alfares W, Bouguennec A, Balfourier F, Gay G, Berges H, Vautin S, Sourdille P, Bernard M, Feuillet C (2009) Fine mapping and marker development for the crossability gene SKr on chromosome 5BS of hexaploid wheat (Triticum aetivum L.). Genetics 183:469–481

    Article  PubMed  CAS  Google Scholar 

  • Bansal UK, Hayden MJ, Gill MB, Bariana HS (2010) Molecular mapping of a seedling stripe rust resistance gene in wheat cultivar Rubric. Euphytica 171:121–127

    Article  Google Scholar 

  • Bariana HS, Willey NJ, Venkata BP, Lehmenseik A, Standen GE, Lu M (2004) Breeding methodology to achieve durability for rust resistance in wheat. In: Black CK, Panozzo JF, Rebetzke GJ (eds) Proceedings of the 54th Australian cereal chemistry conference and 11th wheat breeders assembly. Canberra, ACT, pp 8–12

  • Bariana HS, Brown GN, Bansal UK, Miah H, Standen GE, Lu M (2007) Breeding triple rust resistant wheat cultivars for Australia using conventional and marker-assisted selection technologies. Aust J Agric Res 58:576–587

    Article  Google Scholar 

  • Cao S, Carver BF, Zhu X, Fang T, Chen Y, Hunger RM, Yan L (2010) A single nucleotide polymorphism that accounts for allelic variation in the Lr34 gene and leaf rust reaction in hard winter wheat. Theor Appl Genet. doi:10.1007/s00122-010-1317-6

  • Dakouri A, McCallum BD, Wlichnowski AZ, Cloutier S (2010) Fine mapping of the leaf rust Lr34 locus in Triticum aestivum (L.) and characterization of large germplasm collections support the ABC transporter as essential for gene function. Theor Appl Genet. doi:10.1007/s00122-010-1316-7

  • Dyck PL (1994) Genetics of leaf rust resistance in 13 accessions of the Watkins wheat collection. Euphytica 80:151–155

    Article  Google Scholar 

  • Dyck PL, Jedel PE (1989) Genetics of resistance to leaf rust in two accessions of common wheat. Can J Plant Sci 69:531–534

    Article  Google Scholar 

  • German S, Barcellos A, Chaves M, Kohli M, Campos P, de Viedma L (2007) The situation of common wheat rusts in the Southern Cone of America and perspectives for control. Aust J Agric Res 58:620–630

    Article  Google Scholar 

  • Hayden MJ, Nguyen TM, Waterman A, McMichael GL, Chalmers KJ (2008) Application of multiplex-ready PCR for fluorescence-based SSR genotyping in barley and wheat. Mol Breed 21:271–281

    Article  CAS  Google Scholar 

  • Hiebert C, Thomas J, McCallum B (2005) Locating the broadspectrum wheat leaf rust resistance gene Lr52 (LrW) to chromosome 5B by a new cytogenetic method. Theor Appl Genet 110:1453–1457

    Article  PubMed  CAS  Google Scholar 

  • Kolmer JA, Jin Y, Long DL (2007) Wheat leaf and stem rust in the United States. Aust J Agric Res 58:631–638

    Article  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugenics 12:172–175

    Article  Google Scholar 

  • Krattinger SG, Lagudah ES, Speilmeyer W, Singh RP, Heurto-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science. doi:10.1126/1166453

  • Lugudah ES, McFadden H, Singh RP, Huerta-Espino J, Bariana HS, Spielmeyer W (2006) Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in Wheat. Theor Appl Genet 114:21–30

    Article  Google Scholar 

  • Manly KF, Cudmore RH Jr, Meer JM (2001) Map manager QTX, cross-platform software for genetic mapping. Mammal Genome 12:930–932

    Article  CAS  Google Scholar 

  • McCallum BD, Fetch T, Chong J (2007) Cereal rust control in Canada. Aust J Agric Res 58:639–647

    Article  Google Scholar 

  • McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publishing, Melbourne, p 199

    Google Scholar 

  • McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ, Appels R, Somers DJ, Anderson J (2008) Catalogue of gene symbols for wheat. In: Appels R, Eastwood R, Lagudah E, Langridge P, Mackay M, McIntyre L, Sharp P (eds) Proceedings of the 11th International wheat genetics symposium, Brisbane, Australia, p 59

  • Obert DE, Fritz AK, Moran JL, Singh S, Rudd JC, Menz MA (2005) Identification and molecular tagging of a gene from PI 289824 conferring resistance to leaf rust (Puccinia triticina) in wheat. Theor Appl Genet 110:1439–1444

    Article  PubMed  CAS  Google Scholar 

  • Paillard S, Schnurbusch T, Abderhalden O, Messmer M, Sourdille P, Winzeler M et al (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.). Theor Appl Genet 107:1235–1242

    Article  PubMed  CAS  Google Scholar 

  • Peleg Z, Saranga Y, Suprunova T, Ronin Y, Röder MS, Kilian A, Korol AB, Fahima T (2008) High-density genetic map of durum wheat × wild emmer wheat based on SSR and DArT markers. Theor Appl Genet 117:103–115

    Article  PubMed  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Tar M, Purnhauser L, Csosz M (2008) Identification and localisation of molecular markers linked to the Lr52 leaf rust resistance gene of wheat. Cereal Res Commun 36:409–415

    Article  CAS  Google Scholar 

  • Wellings CR (2007) Puccinia striiformis in Australia: a review of the incursion, evolution and adaptation of stripe rust in the period 1979–2005. Aust J Agric Res 58:567–575

    Article  Google Scholar 

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Acknowledgments

We thank Mr Greg Grimes, Australian Winter Cereal Collection, Tamworth, for provision of seed and relevant information and Dr Mike Bonman, USDA, for information related to PI 289824. Financial support by the GRDC Australia is gratefully acknowledged.

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Authors and Affiliations

  1. The University of Sydney Plant Breeding Institute-Cobbitty, Faculty of Agriculture, Food and Natural Resources, Private Bag 4011, Narellan, NSW, 2567, Australia

    U. K. Bansal, H. Miah, D. Singh & H. S. Bariana

  2. Department of Primary Industries Victoria, Victorian AgriBioscience Centre, La Trobe Research and Development Park, Bundoora, VIC, 3083, Australia

    K. L. Forrest & M. J. Hayden

Authors
  1. U. K. Bansal
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  2. K. L. Forrest
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  3. M. J. Hayden
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  4. H. Miah
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  5. D. Singh
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  6. H. S. Bariana
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Corresponding author

Correspondence to H. S. Bariana.

Additional information

Communicated by J. Dubcovsky.

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Bansal, U.K., Forrest, K.L., Hayden, M.J. et al. Characterisation of a new stripe rust resistance gene Yr47 and its genetic association with the leaf rust resistance gene Lr52 . Theor Appl Genet 122, 1461–1466 (2011). https://doi.org/10.1007/s00122-011-1545-4

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  • DOI: https://doi.org/10.1007/s00122-011-1545-4

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