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Mapping QTL for dollar spot resistance in creeping bentgrass (Agrostis stolonifera L.)

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Abstract

Dollar spot caused by Sclerotinia homoeocarpa F. T. Bennett is the most economically important turf disease on golf courses in North America. Dollar spot resistance in a creeping bentgrass cultivar would greatly reduce the frequency, costs, and environmental impacts of fungicide application. Little work has been done to understand the genetics of resistance to dollar spot in creeping bentgrass. Therefore, QTL analysis was used to determine the location, number and effects of genomic regions associated with dollar spot resistance in the field. To meet this objective, field inoculations using a single isolate were performed over 2 years and multiple locations using progeny of a full sib mapping population ‘549 × 372’. Dollar spot resistance seems to be inherited quantitatively and broad sense heritability for resistance was estimated to be 0.88. We have detected one QTL with large effect on linkage group 7.1 with LOD values ranging from 3.4 to 8.6 and explaining 14–36% of the phenotypic variance. Several smaller effect QTL specific to rating dates, locations and years were also detected. The association of the tightly linked markers with the LG 7.1 QTL based on 106 progeny was further examined by single marker analysis on all 697 progeny. The high significance of the QTL on LG 7.1 at a sample size of 697 (P < 0.0001), along with its consistency across locations, years and ratings dates, indicated that it was stable over environments. Markers tightly linked to the QTL can be utilized for marker-assisted selection in future bentgrass breeding programs.

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Acknowledgments

We thank Dr. Tom Voigt for maintaining the turf research plot at University of Illinois Urbana-Champaign. Funding for this project was provided by Hatch Formula Fund (WIS04777), the Wisconsin Turfgrass Association, U.S. Department of Agriculture (USDA) Research Cooperative Agreement, and United States Golf Association (USGA).

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Authors and Affiliations

  1. Department of Crop Sciences, University of Illinois, Urbana Champaign, IL, 61801, USA

    N. Chakraborty & J. Curley

  2. USDA-ARS, Floral and Nursery Plants Research Unit, Beltsville, MD, 20705, USA

    S. Warnke

  3. USDA-ARS, US Dairy Forage Research Center, Madison, WI, 53706, USA

    M. D. Casler

  4. Department of Plant, Soil and Insect Sciences, University of Massachusetts, Amherst, MA, 01003, USA

    G. Jung

Authors
  1. N. Chakraborty
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  2. J. Curley
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  3. S. Warnke
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  4. M. D. Casler
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  5. G. Jung
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Corresponding author

Correspondence to G. Jung.

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Communicated by F. von Eeuwijk.

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Chakraborty, N., Curley, J., Warnke, S. et al. Mapping QTL for dollar spot resistance in creeping bentgrass (Agrostis stolonifera L.). Theor Appl Genet 113, 1421–1435 (2006). https://doi.org/10.1007/s00122-006-0387-y

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  • DOI: https://doi.org/10.1007/s00122-006-0387-y

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