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Application of a new IBD-based QTL mapping method to common wheat breeding population: analysis of kernel hardness and dough strength

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Abstract

Mapping quantitative trait loci (QTL) in plants is usually conducted using a population derived from a cross between two inbred lines. The power of such QTL detection and the estimation of the effects highly depend on the choice of the two parental lines. Thus, the QTL found represent only a small part of the genetic architecture and can be of limited economical interest in marker-assisted selection. On the other hand, applied breeding programmes evaluate large numbers of progeny derived from multiple-related crosses for a wide range of agronomic traits. It is assumed that the development of statistical techniques to deal with pedigrees in existing plant populations would increase the relevance and cost effectiveness of QTL mapping in a breeding context. In this study, we applied a two-step IBD-based-variance component method to a real wheat breeding population, composed of 374 F6 lines derived from 80 different parents. Two bread wheat quality related traits were analysed by the method. Results obtained show very close agreement with major genes and QTL already known for those two traits. With this new QTL mapping strategy, inferences about QTL can be drawn across the breeding programme rather than being limited to the sample of progeny from a single cross and thus the use of the detected QTL in assisting breeding would be facilitated.

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Acknowledgements

The authors thank the two reviewers for helpful comments on the manuscript, notably for the description of the ‘disruptive selection’. They are grateful to B. Duperrier, J. B. Beaufume, and J. Stragliatti for providing the wheat breeding material and to E. Chanliaud and F. X. Oury for providing the quantitative traits. They are also grateful to M. Joffre and B. Servin for software development. Microsatellites data were produced on the INRA Clermont-Ferrand genotyping platform. This research was supported by the Ministère de l’Economie, des Finances et de l’Industrie (ASG Program No. 01 04 90 6058) and by the “Food Quality” programme, jointly founded by the INRA, the Auvergne Region and the French ministries of Research and of Agriculture.

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Authors and Affiliations

  1. UMR 1095 INRA-UBP, 234 Av. du Brezet, 63039, Clermont-Ferrand Cedex, France

    Sebastien Crepieux & Gilles Charmet

  2. Limagrain, site d’ULICE, av G. Gershwin, BP173, 63204, Riom Cedex, France

    Sebastien Crepieux, Claude Lebreton & Pascal Flament

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  1. Sebastien Crepieux
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  2. Claude Lebreton
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  3. Pascal Flament
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  4. Gilles Charmet
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Correspondence to Sebastien Crepieux.

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Communicated by A. Charcosset

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Crepieux, S., Lebreton, C., Flament, P. et al. Application of a new IBD-based QTL mapping method to common wheat breeding population: analysis of kernel hardness and dough strength. Theor Appl Genet 111, 1409–1419 (2005). https://doi.org/10.1007/s00122-005-0073-5

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  • DOI: https://doi.org/10.1007/s00122-005-0073-5

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