Abstract
Anthracnose fruit rot is an economically important disease that affects pepper production in Indonesia. Strong resistance to two causal pathogens, Colletotrichum gloeosporioides and C. capsici, was found in an accession of Capsicum chinense. The inheritance of this resistance was studied in an F2 population derived from a cross of this accession with an Indonesian hot pepper variety (Capsicum annuum) using a quantitative trait locus (QTL) mapping approach. In laboratory tests where ripe fruits were artificially inoculated with either C. gloeosporioides or C. capsici, three resistance-related traits were scored: the infection frequency, the true lesion diameter (averaged over all lesions that actually developed), and the overall lesion diameter (averaged over all inoculation points, including those that did not develop lesions). One main QTL was identified with highly significant and large effects on all three traits after inoculation with C. gloeosporioides and on true lesion diameter after inoculation with C. capsici. Three other QTL with smaller effects were found for overall lesion diameter and true lesion diameter after inoculation with C. gloeosporioides, two of which also had an effect on infection frequency. Interestingly, the resistant parent carried a susceptible allele for a QTL for all three traits that was closely linked to the main QTL. The results with C. capsici were based on less observations and therefore less informative. Although the main QTL was shown to have an effect on true lesion diameter after inoculation with C. capsici, no significant QTL were identified for overall lesion diameter or infection frequency.
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Acknowledgements
The SSR primers were kindly provided by Dr. I. Nagy (Agricultural Biotechnology Center, Gödöllö, Hungary). The Seq1 primer for NBS profiling was developed by J. Mes of Plant Research International, Wageningen, The Netherlands. The work of Lia Sanjaya was made possible by a grant of the Royal Netherlands Academy of Arts and Sciences (KNAW).
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Voorrips, R.E., Finkers, R., Sanjaya, L. et al. QTL mapping of anthracnose (Colletotrichum spp.) resistance in a cross between Capsicum annuum and C. chinense. Theor Appl Genet 109, 1275–1282 (2004). https://doi.org/10.1007/s00122-004-1738-1
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DOI: https://doi.org/10.1007/s00122-004-1738-1