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Inhibitory effect of Gymnema Montanum leaves on α-glucosidase activity and α-amylase activity and their relationship with polyphenolic content

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Abstract

The present study was attempted to investigate the effect of G. montanum leaf extract on inhibition of α-glucosidase and α-amylase activity. The ethanol extract of G. montanum (GLEt) at various concentrations (1–10 μg/ml) was tested for its inhibition pattern against α-glucosidase and α-amylase activity in vitro and compared with the commercially available α-glucosidase inhibitor, acarbose. The GLEt showed competitive inhibition against yeast α-glucosidase and noncompetitive inhibition against salivary α-amylase in a concentration-dependent manner. Further, we investigated the effect of extract on levels of blood glucose and plasma insulin in neonatal streptozotocin-induced type 2 diabetic rats. Long-term administration (12 weeks) of the GLEt effectively reduced the blood glucose level and also increased the insulin level. In a preliminary phytochemical analysis, G. montanum leaves were rich in phenolic composition and found to be positively correlated with the inhibitory effect of α-glucosidase and α-amylase activity. These results suggest that the GLEt might exert its antidiabetic effect by suppressing carbohydrate absorption from the intestine and thereby reducing hyperglycemia.

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Acknowledgment

K. M. Ramkumar is grateful to the Council of Scientific and Industrial Research, New Delhi, for awarding him a Senior Research Fellowship.

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Authors and Affiliations

  1. Department of Biotechnology, Anna University Tiruchirappalli, Tiruchirappalli, 620 024, Tamil Nadu, India

    Kunga Mohan Ramkumar & Palanisamy Rajaguru

  2. Department of Biochemistry, Centre for Plant Molecular Biology, Tamilnadu Agricultural University, Coimbatore, 641 003, India

    Balsamy Thayumanavan

  3. Department of Biochemistry, Periyar University, Salem, 636 011, India

    Thayumanavan Palvannan

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  1. Kunga Mohan Ramkumar
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  2. Balsamy Thayumanavan
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  3. Thayumanavan Palvannan
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  4. Palanisamy Rajaguru
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Correspondence to Kunga Mohan Ramkumar.

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Ramkumar, K.M., Thayumanavan, B., Palvannan, T. et al. Inhibitory effect of Gymnema Montanum leaves on α-glucosidase activity and α-amylase activity and their relationship with polyphenolic content. Med Chem Res 19, 948–961 (2010). https://doi.org/10.1007/s00044-009-9241-5

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