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The effects of various doses of bacterial lipopolysaccharide on the expression of CD63 and the release of histamine by basophils of atopic and non-atopic patients

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Abstract

Objective

We tested the effect of various doses of bacterial lipopolysaccharide (LPS, endotoxin) on the expression of CD63 and the in vitro release of histamine by basophils stimulated with ragweed allergen in patients with or without ragweed and mite allergies.

Methods

The peripheral blood of 11 patients with ragweed allergy, 10 patients with mite allergy and 14 control patients was incubated with ragweed allergen extract following pretreatment with varying doses of LPS. The expression of CD63 in basophils was measured by flow cytometry, and the release of histamine was determined by ELISA.

Results

In the samples of patients with ragweed allergy that were exposed to specific allergen, only high doses of LPS significantly elevated the expression of CD63 (200 ng/ml; 1,000 EU/ml) and the release of histamine (2,000 ng/ml; 10,000 EU/ml). There was no effect of LPS in any other cases.

Conclusions

Bacterial LPS (endotoxin) concentrations higher than 200 ng/ml (1,000 EU/ml), which rarely occurs in nature, could only activate the basophils from atopic patients whilst in the presence of the specific allergen. Thus, the restoration of the urban, “microbe-poor” milieu with endotoxin (as LPS) can be a promising and harmless approach for allergy prevention.

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References

  1. Akira S, Takeda K, Kaisho T. Toll-like receptors: critical proteins linking innate and acquired immunity. Nat Immunol. 2001;2:675–80.

    Article  PubMed  CAS  Google Scholar 

  2. Gereda JE, Leung DY, Thatayatikom A, Streib JE, Price MR, Klinnert MD, et al. Relation between house-dust endotoxin exposure, type 1 T-cell development, and allergen sensitization in infants at high risk of asthma. Lancet. 2000;355:1680–3.

    Article  PubMed  CAS  Google Scholar 

  3. Braun-Fahrländer C, Riedler J, Herz U, Eder W, Waser M, Grize L, et al. Allergy and endotoxin study team: environmental exposure to endotoxin and its relation to asthma in school-age children. N Engl J Med. 2002;347:869–77.

    Article  PubMed  Google Scholar 

  4. Gehring U, Bischof W, Fahlbush B, Wichman H, Heinrich J. House dust endotoxin and allergic sensitization in children. Am J Respir Crit Care Med. 2002;166:939–44.

    Article  PubMed  Google Scholar 

  5. Van Strien RT, Engel R, Holt O, Bufe A, Eder W, Waser M, et al. ALEX study team: microbial exposure of rural school children, as assessed by levels of N-acetyl-muramic acid in mattress dust, and its association with respiratory health. J Allergy Clin Immunol. 2004;113:860–7.

    Article  PubMed  Google Scholar 

  6. Gehring U, Bischof W, Schlenvoight G, Richter K, Fahlbush B, Wischmann HE, et al. INGA study group: exposure to house dust endotoxin and allergic sensitization in adults. Allergy. 2004;59:946–52.

    Article  PubMed  CAS  Google Scholar 

  7. Park JH, Gold DR, Spiegelman DL, Burge HA, Milton DK. House dust endotoxin and repeated wheeze in the first year of life. Am J Respir Crit Care Med. 2001;163:322–8.

    PubMed  CAS  Google Scholar 

  8. Rizzo MC, Naspitz CK, Fernandez-Caldas E, Lockey RF, Mimica I, Sole D. Pediatr Allergy Immunol. 1997;8:121–6.

    Article  PubMed  CAS  Google Scholar 

  9. Norn S, Baek L, Jensen C, Stahl Skov P, Permin H, Jarlov JO, et al. Influence of bacterial endotoxins on basophil histamine release. Allergy. 1986;41:125–30.

    Article  PubMed  CAS  Google Scholar 

  10. Clementsen P, Norn S, Kristensen KS, Bach-Mostensen N, Koch C, Permin H. Bacteria and endotoxin enhance basophil histamine release and potentiation is abolished by carbohydrates. Allergy. 1990;45:402–8.

    Article  PubMed  CAS  Google Scholar 

  11. Sheehan WJ, Hoffman EB, Fu C, Baxi SN, Bailey A, King EM, et al. Endotoxin exposure in inner-city schools and homes of children with asthma. Ann Allergy Asthma Immunol. 2012;108:418–22.

    Article  PubMed  CAS  Google Scholar 

  12. Gyimesi E, Sipka S, Dankó K, Kiss E, Hídvégi B, Gál M, et al. Basophil CD63 expression assay on highly sensitized atopic donor leukocytes—a useful method in chronic autoimmune urticaria: british. J Dermatol. 2004;151:388–96.

    Article  CAS  Google Scholar 

  13. Ehlers S, Kaufmann SH. In: 99th Dahlem conference on infection, inflammation and chronic inflammatory disorders: lifestyle changes affecting the host-environment interface. Clin Exp Immunol. 2010;160:10–4.

  14. Matricardi PM, Bjorksten B, Bonini S, Bousquet J, Djukanovic R, Dreborg S, et al. EAACI task force 7: microbial products in allergy prevention and therapy. Allergy. 2003;58:461–71.

    Article  PubMed  CAS  Google Scholar 

  15. De Weck AL, Sanz ML, Gamboa PM, Aberer W, Bienvenu J, Blanca M, et al. Diagnostic tests based on human basophils: more potentials and perspectives than pitfalls. Int Arch Allergy Immunol II. 2008;146:177–89.

    Article  Google Scholar 

  16. Chirumbolo S, Vella A, Ortolani R, De Gironcoli M, Solero P, Tridente G, et al. Differential response of human basophil activation markers: a multiparameter flow cytometry approach. Clin Mol Allergy. 2008;6:12.

    Article  PubMed  Google Scholar 

  17. Ebo DG, Sainte-Laudy J, Bridts CH, Mertens CH, Hagendorens MM, Schuerwegh AJ, et al. Flow-assisted allergy diagnosis: current applications and future perspectives. Allergy. 2006;61:1028–39.

    Article  PubMed  CAS  Google Scholar 

  18. Knol EF, Mul FP, Jansen H, Calafat J, Roos D. Monitoring human basophil activation via CD63 monoclonal antibody 435. J Allergy Clin Immunol. 1991;88:328–38.

    Article  PubMed  CAS  Google Scholar 

  19. Hennesrsdorf F, Florian S, Jakob A, Baumgartner K, Somneck K, Nordheim A, et al. Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Res. 2005;15:325–35.

    Article  Google Scholar 

  20. MacGlashan D. Expression of CD203c and CD63 in basophils: relationship to differential regulation of piecmeal and anaphylactic degranulation processes. Clin Exp Allergy. 2010;201(40):1365–77.

    Article  Google Scholar 

  21. Mendy A, Gasana J, Vieira ER, Forno E, Patel J, Kadam P, et al. Endotoxin exposure and childhood wheeze and asthma: a meta-analysis of observational studies. J Asthma. 2011;48:685–93.

    Article  PubMed  CAS  Google Scholar 

  22. Chang AB, Peake J, McElrea MS (2008) Anti-histamines for prolonged non-specific cough in children. Cochrane Database Syst Rev 16(2): CD005604.

    Google Scholar 

  23. Ngamphaiboon J, Wirawarn Z, Thongkaew T. Prevention of recurrent wheezing in young children by loratidine compared with ketotifen. J Med Assoc Thai. 2009;92:351–5.

    PubMed  Google Scholar 

  24. Rennie DC, Lawson JA, Senthilselvan A, Wiltson PJ, Dosman A. Domestic endotoxin exposure and asthma in children: epidemiological studies. Front Biosci. 2012;4:56–73.

    Google Scholar 

  25. Senthilselvan A, Dosman JA, Chénard L, Bruch LH, Predicala BZ, Sorowski R, et al. Toll-like receptor 4 variants reduce airway response in human subjects at high endotoxin levels in a swine facility. J Allergy Clin Immunol. 2009;123:1034–40.

    Article  PubMed  CAS  Google Scholar 

  26. Park JH, Spiegelman DL, Burge HA, Gold DR, Chew GL, Milton DK. Longitudinal study of dust and airborne endotoxin in the home. Environ Health Perspect. 2000;108:1023–8.

    Article  PubMed  CAS  Google Scholar 

  27. Abbing-Karahapopian V, van der Gugten AC, van der Ent CK, Uiterwaal C, de Jongh M, Oldenwening M, et al. Effect of endotoxin and allergens on neonatal lung function and infancy respiratory symtoms and eczema. Pediatr Allergy Immunol. 2012;5:448–55.

    Article  Google Scholar 

  28. Basisnas I, Schlünssen V, Heederik D, Sigsgaard T, Smit LA, Samadi S, et al. Sensitisation to common allergens and respiratory symptoms in endotoxin exposed workers: a pooled analysis. Occup Environ Med. 2012;69:99–106.

    Article  Google Scholar 

  29. Babolewska E, Witczak P, Pietrzak A, Brzezinska-Blaszczyk E. Different potency of bacterial antigens TLR2 and TLR4 ligands in stimulating mature mast cells to cyteinyl leukotriene synthesis. Microbiol Immunol. 2012;56:183–90.

    Article  PubMed  CAS  Google Scholar 

  30. Brekke OL, Christiansen D, Fure H, Fung M, Mollnes TE. The role of complement 3 opsonization, C5a receptor, and CD14 in E.coli-induced up regulation of granulocyte and monocyte CD11b/CD18 (CR3), phagocytosis, and oxidative burst in human whole blood. J Leukoc Biol. 2007;81:1404–13.

    Article  PubMed  CAS  Google Scholar 

  31. Guan W, Zheng J, Gao Y, Jiang C, An J, Yu X, et al. Leukotriene D4 bronchial provocation test: methodology and diagnostic value. Curr Med Res. 2002;28:797–803.

    Article  Google Scholar 

  32. Hrncir T, Stepankova R, Kozakova H, Hudcovic T, Tlaskolava-Hogenova H. Gut microbiota and lipopolysaccharide content of the diet influence development of regulatory T cells: studies in germ-free mice. BMC Immunol. 2008;9:65–72.

    Article  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the grant of the Hungarian Research Found OTKA 71883. The authors thank Mrs. Ibolya Szöllősi and Mrs. Deákné Anikó Tormás for their skillful technical assistance.

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Authors and Affiliations

  1. Division of Clinical Immunology, Department of Medicine, Medical and Health Science Centre, University of Debrecen, Móricz Zs U. 22, Debrecen, 4032, Hungary

    E. Gyimesi, G. Pál, S. Baráth & S. Sipka

  2. County Hospital, Debrecen, Hungary

    F. Gönczi

  3. Department of Pulmonology, Medical and Health Sciences Center, University of Debrecen, Debrecen, Hungary

    M. Szilasi

Authors
  1. E. Gyimesi
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  2. F. Gönczi
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  3. M. Szilasi
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  4. G. Pál
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  5. S. Baráth
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  6. S. Sipka
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Correspondence to S. Sipka.

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Responsible Editor: Andras Falus.

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Gyimesi, E., Gönczi, F., Szilasi, M. et al. The effects of various doses of bacterial lipopolysaccharide on the expression of CD63 and the release of histamine by basophils of atopic and non-atopic patients. Inflamm. Res. 62, 213–218 (2013). https://doi.org/10.1007/s00011-012-0569-9

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  • DOI: https://doi.org/10.1007/s00011-012-0569-9

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