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Circulating blood monocytes traffic to and participate in the periprosthetic tissue inflammation

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Abstract

Objective

To examine the trafficking of human circulating blood monocytes and their influence on the inflammation of periprosthetic tissues using a novel mouse–human chimera model.

Methods

Periprosthetic tissue and bone chips from patients with aseptic prosthetic loosening were implanted into the muscles of immune-deficient SCID mice depleted of host macrophages by periodic intraperitoneal injection of anti-asialo GM1 rabbit sera (ASGM1). Autologous patient peripheral blood monocytes (PBMCs) were labeled with PKH2 fluorescent dye and injected intraperitoneally into the implanted animals. Mice were sacrificed 14 days after PBMC transfusion for molecular and histological analyses.

Results

Patient periprosthetic tissues were well tolerated in SCID mice and preserved a high level of viability. Cell trafficking studies revealed the accumulation of fluorescent PBMC within the xenografts, with total cell counts in the xenografts significantly increased following the systemic PBMC infusion. PBMC infusion also promoted the expression of IL-1, IL-6, TNFα, and RANK within the periprosthetic tissue.

Conclusion

Systemic PBMC migrated to the implanted periprosthetic tissues and contributed to the local inflammation. The data provide evidence that circulating blood monocytes may play a role in pathologic process during aseptic loosening of total joint replacement.

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Acknowledgments

We thank Ms. Zheng Song, Bin Wu, and Li Mao for their excellent technical assistance. This work was supported by research grants from Orthopaedic Research and Education Foundation (OREF, S.-Y.Y.), NIH (5R03AR054929-02, S-Y.Y.), and Veteran Administration (Merit Award, P.H.W.).

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Authors and Affiliations

  1. Shandong University School of Medicine, 250012, Jinan, Shandong, China

    Kai Zhang, Tang-Hong Jia & Wei-Ming Gong

  2. Orthopaedic Research Institute, Via Christi Regional Medical Center, 929 N Saint Francis, Wichita, KS, 67214, USA

    Kai Zhang, David McQueen, Paul H. Wooley & Shang-You Yang

  3. Department of Biological Sciences, Wichita State University, Wichita, KS, USA

    Paul H. Wooley & Shang-You Yang

  4. Department of Orthopaedic Surgery, Providence Hospital, Southfield, MI, USA

    David C. Markel

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  1. Kai Zhang
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  2. Tang-Hong Jia
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  3. David McQueen
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  4. Wei-Ming Gong
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  5. David C. Markel
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  6. Paul H. Wooley
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  7. Shang-You Yang
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Correspondence to Shang-You Yang.

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Responsible Editor: A. Bauhofer.

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Zhang, K., Jia, TH., McQueen, D. et al. Circulating blood monocytes traffic to and participate in the periprosthetic tissue inflammation. Inflamm. Res. 58, 837–844 (2009). https://doi.org/10.1007/s00011-009-0051-5

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  • DOI: https://doi.org/10.1007/s00011-009-0051-5

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