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Detection of galectin-3 in patients with inflammatory bowel diseases: new serum marker of active forms of IBD?

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Abstract

Objective

It is an open question whether multifunctional galectin-3 can be a serum marker in inflammatory bowel disease.

Methods

Western blots and commercial ELISA detected and quantitated the lectin immunocytochemistry using double labeling localized it in tissue sections.

Results

Serum concentrations were significantly increased in specimen of patients with active and remission-stage ulcerative colitis and Crohn’s disease, associated with emerging positivity of CD14+ cells.

Conclusion

Enhanced concentration of galectin-3 in serum reflects presence of disease and points to its involvement in the pathogenesis.

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Acknowledgments

This work was supported by the Czech Science Foundation (project nos. 310/08/H077 and 303/060/974), the Academy of Sciences (project no. S500200572), the Ministry of Education, Youth and Sports of the Czech Republic (projects no. 2B06155, no. MSM0021620806 and 1M0538), the Institute of Microbiology (project no. AV0Z50200510), the Ministry of Health (project no. NR8963-3), the research initiative LMUexcellent, the Verein zur Förderung des biologisch-technologischen Fortschritts in der Medizin e. V. and an EU Marie Curie Research Training Network grant (contract no. MCRTN-CT-2005-19561). The authors are grateful to B. Hofmanová and I. Burdová for excellent technical assistance, Dr. B. Friday and Dr. S. Namirha for valuable discussion and to the reviewers of this manuscript for their inspiring advice.

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Authors and Affiliations

  1. Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, Prague 4, 142 20, Czech Republic

    Lenka Frol’ová, Dana Borovská, Andrea Kitanovičová, Klára Klimešová, Ludmila Tučková & Helena Tlaskalová-Hogenová

  2. 1st Faculty of Medicine, Charles University, Karlovo nam. 32, 121 11, Prague 2, Czech Republic

    Helena Tlaskalová-Hogenová

  3. Institute of Anatomy and Center of Cell Therapy and Tissue Repair, 1st Faculty of Medicine, Charles University, U nemocnice 3, 128 00, Prague 2, Czech Republic

    Karel Smetana Jr

  4. Institute of Clinical Biochemistry and Laboratory Diagnostics, General Faculty Hospital, 1st Faculty of Medicine, Charles University, Karlovo nam. 32, 121 11, Prague 2, Czech Republic

    Ivana Janatková, Karin Malíčková & Milan Lukáš

  5. Department of Gastroenterology and Hepatology, Institute of Clinical and Experimental Medicine, Vídeňská 1958/9, 140 21, Prague 4, Czech Republic

    Pavel Drastich

  6. 2nd Internal Clinic, Teaching Thomayer Hospital, Vídeňská 800, 140 59, Prague 4, Czech Republic

    Zdeněk Beneš

  7. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstrasse 13, 80539, Munich, Germany

    Joachim C. Manning, Sabine André & Hans-Joachim Gabius

  8. IBD Clinical and Research Centre, ISCARE IVF, Jankovcova 1569/2c, 170 04, Prague 7, Czech Republic

    Milan Lukáš

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  1. Lenka Frol’ová
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  14. Hans-Joachim Gabius
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Correspondence to Helena Tlaskalová-Hogenová.

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Responsible Editor: I. Ahnfelt-Rønne.

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Frol’ová, L., Smetana, K., Borovská, D. et al. Detection of galectin-3 in patients with inflammatory bowel diseases: new serum marker of active forms of IBD?. Inflamm. Res. 58, 503–512 (2009). https://doi.org/10.1007/s00011-009-0016-8

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  • DOI: https://doi.org/10.1007/s00011-009-0016-8

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