Abstract
By means of cDNA-RDA method, some cDNA fragments were found to have high levels of expression during deprivation of GM-CSF (granulocyte macrophage-colony stimulating factor) in a human myeloid cell line, TF-1 cells. One of these fragments was identified as a novel gene. To get the full length of cDNA, rapid amplification of cDNA ends (RACE) and expressed sequence tags (EST) overlapping fragments assembling strategies were used. The novel gene was named TRAF15 (TF-1 cell apoptosis related gene-15), which consists of 1 218 nucleotides and encodes 212 amino acids. The putative protein product of TFAR15 is partially homologous toC. elegans protein C14A4.11. TFAR15 mRNA is expressed in fetal liver, kidney, spleen and lung, and also in some human myeloid cell lines. Both of the TFAR15 mRNA and protein were highly expressed in TF-1 cells after GM-CSF withdrawal.In vitro analysis showed that the recombinant TFAR15 protein could inhibit the natural cell death of 293 cells, an embryonic kidney cell line.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Steller, H., Mechanisms and genes of cellular suicide,Science, 1995, 267: 1445.
Russ, A. P., Riedel, C., Ballas, K. et al., Identification of genes induced by factor deprivation in hematopoietic cells undergoing apoptosis using gene-trap mutagenesis and site-specific recombination,Proc. Natl. Acad. Sci. USA, 1996, 93: 15279.
Gaozza, E., Baker, S. J., Vora, R. K. et al., AATYK: a novel tyrosine kinase induced during growth arrest and apoptosis of myeloid cells,Oncogene, 1997, 15: 3127.
Lotem, J., Sachs, L., Differential Suppression by protease inhibitors and cytokines of apoptosis induced by will-type p53 and cytotoxic agents,Proc. Natl. Acad. Sci. USA, 1996, 93: 12507.
Hubank, M., Schatz, D. G., Identifying differences in mRNA expression by representational difference analysis of cDNA,Nucleic Acids Res., 1995, 22: 5640.
Sambrook, J., Fritsch, E. F., Maniatis, T.,Molecular Cloning: A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989, 7.39–7.56.
Zhou, B. H., Ma, D. L., Di, C. H. et al., Amplification of cDNA encoding human monocyte-derived interlukin-8 by PCR and its overexpression inE.coli, Chin. J. Microbiol. & Immunol., 1992, 12: 174.
Coligan, J. E., Kruisbeek, A. M., Margulies, D. H. et al.,Current Protocols in Immunology, New York: Greene Publishing Associates and Wiley-Interscience, 1991, 5.7.1–5.7.4.
Sambrook, J., Fritsch, E. F., Maniatis, T.,Molecular Cloning: A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989, 18.3–18.74.
Adams, M. D., Kelley, J. M., Gocayne, J. D. et al., Complementary DNA sequencing: expressed sequence tags and human genome project,Science, 1991, 252: 1651.
Pawlak, A., Toussaint, C., Levy, I. et al., Characterization of a large population of mRNAs from human testis,Genomics, 1995, 26: 151.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wang, Y., Liu, H., Zhang, Y. et al. cDNA cloning and expression of an apoptosis-related gene, humanTFAR15 gene. Sci. China Ser. C.-Life Sci. 42, 323–329 (1999). https://doi.org/10.1007/BF03183610
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03183610