Abstract
A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydrogen ions, produced by a van de Graaff accelerator, in an energy range of 2.0–3.0 MeV, into an area smaller than that of the nucleus of an individual living cell. The beam is collimated by a borosilicate glass capillary that forms the beam-line exit. An integrated computer program recognizes the cells and locates them one by one over the microbeam exit for irradiation. We present technical details of the CAS-LIBB microbeam facility, particularly on the collimator, hardware, control program, as well as cell irradiation protocols available. Various factors contributing to the targeting and positioning precision are discussed along with accuracy measurement results.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Zirkle, R. E., Advances in biological and medical physics, New York: Academic Press, 1957, 103–146.
Zirkle, R. E., Bloom, W., Irradiation of parts of cells, Science, 1953, 117: 487–493.
Legge, G. J. F., A history of ion microbeams, Nucl. Instr. Meth. B, 1997, 130: 9–19.
Folkard, M., Vojnovic, B., Prise, K. M. et al., A charged-particle microbeam: Part I: Development of an experimental system for targeting cells individually with counted particles, Int. J. Radiat. Biol., 1997, 72: 375–385.
Folkard, M., Vojnovic, B., Hollis, K. J. et al., A charged-particle microbeam: Part II: A single-particle micro-collimation and detection system, Int. J. Radiat. Biol., 1997, 72: 387–395.
Folkard, M., Vojnovic, B., Gilchrist, S. et al., The design and application of ion microbeams for irradiating living cells and tissues, Nucl. Instr. Meth. B., 2003, 210: 302–307.
Randers-Pehrson, G., Geard, C. R., Johnson, G. et al., The Columbia University single-ion microbeam. Radiat. Res., 2001, 156: 210–214.
Wu, L. J., Hei, T. K., Randers-Pehrson, G. et al., Columbia University microbeam: Development of an experimental system for targeting cells indiviually with counted particles, Nucl. Sci. & Tech. 1999, 10(3): 143–148.
Michelet, C., Moretto, Ph., Barberet, Ph. et al., A focused microbeam for targeting cells with counted multiple particles, Radiat. Res., 2002, 158: 370–371.
Greif, K. D., Brede, H. J., Frankenberg, D. et al., The PTB single ion microbeam for irradiation of living cells, Nucl. Instr. Meth. B., 2004, 217: 505–512.
Peng, S. X., Folkard, M., Gilchrist, S., Locke, R. J. et al., Measurements of the targeting accuracy of the Gray laboratory charged-particle microbeam, Nucl. Instr. Meth. B., 2001, 179: 145–150.
Kamiya, T., Yokota, W., Kobayashi, Y. et al., Development of an automated single cell irradiation system combined with a high-energy heavy ion microbeam system, Nucl. Instr. Meth. B., 2001, 181: 27–31.
Bloom, W., Cellular responses, Rev. Modern Phys., 1959, 31: 66–71.
Kadhim, M. A., Transmission of chromosomal instability after plutonium alpha-particle irradiation, Nature, 1992, 355: 738–740.
Brenner, D. J., Hall, E. J., Microbeams: A potent mix of physics and biology, Radiat. Prot. Dosim, 2002, 99: 283–286.
Yu, Z. L., Ion beam and biology science, Physics, 1997, 26: 333–338.
Yu, Z. L., Shao, C. L., Dose effect of the tyrosine sample implanted by a low energy N+ ion beam. Radiat. Phys. Chem., 1994, 43: 349–351.
Hei, T. K., Wu, L. J., Liu, S. X. et al., Mutagenic effects of a single and an exact number of a particles in mammalian cells, Proc. Natl. Acad. Sci. USA, 1997, 94: 3765–3770.
Wu, L. J., Randers-Pehrson, G., Xu, A. et al., Targeted cytoplasmic irradiation with alpha particles induces mutations in mammalian cells, Proc. Natl. Acad. Sci. USA, 1999, 96: 4959–4964.
Yang, J. B., Wu, L. J., Li, L. et al., Sequence analysis of lacZ mulations induced by ion beam irradiation in double-stranded M13mp18DNA, Sci. in China, Ser. C., 1997, 40: 107–112.
Geard, C. R., Randers-Pehrson, G., Marino, S. A. et al., Intra- and intercellular responses after cell site-specific microbeam irradiation, Radiat. Res., 2000, 153: 233.
Hu, Z. W., Yu, Z. L., Wu, L. J., An optimization control program for the ASIPP microbeam. Nucl. Instr. Meth. A., 2003, 507: 617–621.
Wang, X. F., Chen, L. Y., Yu, Z. L. et al., Quantitative single-ion irradiation by ASIPP microbeam, Chin. Phys. Lett., 2004, 21: 821–824.
Wang, X. H., Wang, S. H., Yu, Z. L., ·· Image feature extracting of cells for the ASIPP microbeam,· · Comput. Engin. (in Chinese), 2003, 29 (18): 9–10.··
Wang, X. H., Wang, S. H., Yu, Z. L., District partition in image acquiring f cells and image distinguishing, Comput. Engin. (in Chinese), 2003, 29 (20): 35–37.
Peng, S. X., Wang, S. H., Yu, Z. L., Ion Micro-beam Manipulation: Deposition of given number of particles into the pre-determined position of individual cells, Vac. Sci & Technol. (in Chinese), 1999, 18: 38–46. ··
Zhan, F. R., Yu, Z. L.,··The calculation of the ion trajectory in an accelerating tube,·· High Power Laser and Particle Beams (in Chinese), 2000, 12(1): 103–106.
Zhan, F. R., Yu, Z. L., Hu, C. D. et al.,··Construction of electrostatic accelerator RF ion source, Vac. Sci. & Technol. (in Chinese), 2000, 20(3): 226–228.
Author information
Authors and Affiliations
Corresponding author
Additional information
The names of the co-authors are ordered according to the number of strokes of their surnames in Chinese characters.
About this article
Cite this article
Wang, X., Wang, X., Chen, L. et al. Development of the CAS-LIBB single-particle microbeam for localized irradiation of living cells. Chin.Sci.Bull. 49, 1806–1811 (2004). https://doi.org/10.1007/BF03183404
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03183404