Summary
The extra-vacuolar nucleus is visible in a small percentage of living cells from 72–96 hour wort cultures. The vacuoles show a luminous boundary under dark ground illumination. The details observed in living nuclei could be stained with haematoxylin after fixation in iodine-formaldehyde-acetic acid mixture. The Feulgen-negative nature of the vacuole and the limitation of the Feulgen-positive material to the area bounded by the nuclear membrane would imply that the ‘centrosome’ described by Lindegren and Rafalko (1950) is the real nucleus. The nucleus ofS. bayanus conforms in its structure to those of higher organisms.
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References
Aswathanarayana, N. V...Proc. Ind. Acad. Sci., 1956,43 B, 314.
—————Proc. Ind. Acad. Sci., 1958,47 B, 225.
—— and Subramaniam, M. K...Proc. Ind. Acad. Sci., 1958,47 B,, 263.
Baker, R...Cytological Techniques, Methuen & Co., Ltd., London, 1955.
Darlington, C. D. and La Cour, L. F.Handling of Chromosomes, George Allen & Unwin, Ltd., London, 1950.
Gatenby, J. B. and Cowdry, E. V.Bolles Lee’s Microtomists Vade Mecum, J. & A. Churchill, London, 1928.
Hirano, T....J. Ultrastructure Research, 1962,7, 201.
Johansen, D. A....Plant Microtechnique, McGraw-Hill Book Co., Inc., New York, 1940.
Lindegren, C. C...The Yeast Cell, Its Genetics and Cytology, Educational Pubrs. Inc., St. Louis, Mo., 1949.
—————..Nature, 1962,195, 1225.
————— and Rafalko, M. M.Exptl. Cell Research, 1950,1, 169.
————— and Townsend, G. F.Cytologia, 1954,19, 104.
Lindegrew, C. C. Williams, M. A. and McClary, D. O.Antonie van Leeuwenhoek, 1956,22, 1.
McClary, D. O., Bowers, W. D. and Miller, G. R.J. Bact., 1962,83, 276.
Royan, S...Proc. Ind. Acad. Sci., 1956,44 B, 47.
—————..Proc. Ind. Acad. Sci., 1958a,47 B, 31.
—————..Ibid., 1958b,48 B, 33.
—————..Curr. Sci., 1958c,27, 400.
————— and Subramaniam M. K.,Proc. Ind. Acad. Sci., 1956,43 B, 223.
————— and Subramaniam M. K.,Ibid., 1960,51 B, 205.
Subramaniam, M. K... Ibid., 1960,51 B, 66.
--, Royan, S., Thyagarajan, T. R., Aswathanarayana, N. V. and Subramanyam, S.Ind. Inst. Sci. Golden Jubilee Res. Vol., 1959, p. 96.
Subramanyam, S...J. Ind. Inst. Sci., 1960,42, 85.
Yotsuyanagi, Y...Compt. rend. Acad. Sci., 1960,250, 1522.
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Communicated by M. K. Subramaniam,f.a.sc.
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Aswathanarayana, N.V. The nucleus ofSaccharomyces bayanus . Proc. Indian Acad. Sci. 58, 128–136 (1963). https://doi.org/10.1007/BF03051945
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DOI: https://doi.org/10.1007/BF03051945