Skip to main content
SpringerLink
Log in

Lipid peroxidative damage on malathion exposure in rats

  • Published:
Neurotoxicity Research Aims and scope Submit manuscript

Abstract

Lipid peroxidation is one of the major outcomes of free radical-mediated injury to tissuein vivo, including the central nervous system (CNS). The aim of this study was to examine whether malathion, a commonly used organophosphorus (OP), might induce oxidative stress in cerebrospinal fluid, blood serum and brain structures in male Wistar rats. Malathion was administered intraperitoneally in the doses of 25, 50, 100 and 150 mg/kg for 28 days. Oxidative damage was determined by measuring the thiobarbituric acid reactive species (TBARS) content, as an index of lipid peroxidation. TBARS concentration in the cerebrospinal fluid (CSF) and brain structures were increased, but a decrease in TBARS concentration in serum was observed. The results of the present study suggest the usefulness of TBARS measurement as a good biomarker in the estimation of malathion-induced oxidative stress affecting CSF and brain structures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abdollahi M, M Donyavi, S Pournourmohammadi and M Saadat (2004a) Hyperglycemia associated with increased hepatic glycogen phosphorylase and phosphoenolpyruvate carboxykinase in rats following subchronic exposure to malathion.Comp. Biochem. Physiol. Part C137, 343–347.

    Google Scholar 

  • Abdollahi M, S Mostafalou, S Pournourmohammadi and S Shadnia (2004b) Oxidative stress and cholinesterase inhibition in saliva and plasma of rats following subchronic exposure to malathion.Comp. Biochem. Physiol. Part C137, 29–34.

    Google Scholar 

  • Ahmed RS, V Seth, ST Pasha and BD Banerjee (2000) Influence of dietary ginger(Zingiber officinales Rosc) on oxidative stress induced by malathion in rats.Food Chem. Toxicol. 38, 443–450.

    Article  PubMed  CAS  Google Scholar 

  • Akhgari M, M Abdollahi, A Kebryaeezadeh, R Hosseini and O Sabzevari (2003) Biochemical evidence for free radical induced lipid peroxidation as a mechanism for subchronic toxicity of malathion in blood and liver of rats.Human Exp. Toxicol. 22, 205–211.

    Article  CAS  Google Scholar 

  • Arlt S, A Kontush, I Zerr, C Buhmann, C Jacobi, A Schröter, S Poser and U Beisiegel (2002) Increased lipid peroxidation in cerebrospinal fluid and plasma from patients with Creutzfeldt Jakob disease.Neurobiol. Dis. 10, 150–156.

    Article  PubMed  CAS  Google Scholar 

  • Ayud S, J Verma and N Das (2003) Effect of endosulfan and malathion on lipid peroxidation, nitrite and TNF-α release by rat peritoneal macrophages.Int. Immunopharmacol. 3, 1819–1828.

    Article  Google Scholar 

  • Banerjee BD (1999) The influence of various factors on immune toxicity assessment of pesticide chemicals.Toxicol. Lett. 107, 21–31.

    Article  PubMed  CAS  Google Scholar 

  • Banerjee DB, V Seth, A Bhattacharya, AK Chakraborty and ST Pasha (1998) Oxidative stress in human poisoning cases following malathion and propoxur ingestion.Toxicol. Lett. 95, 58.

    Article  Google Scholar 

  • Banerjee BD, V Seth, A Bhattacharya, ST Pasha and AK Chakraborty (1999) Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers.Toxicol. Lett. 107, 33–47.

    Article  PubMed  CAS  Google Scholar 

  • Beckman JS, I Chen and H. Ischiropaules (1994) Oxidative chemistry of peroxynitrite.Methods Enzymol. 233, 229–240.

    Article  PubMed  CAS  Google Scholar 

  • Blasiak J, P Jaloszynski, A Trzeciak and K Szyfter (1999)In vitro studies on the genotoxicity of the organophosphorus insecticide malathion and its two analogues.Mutat. Res. 445, 275–283.

    PubMed  CAS  Google Scholar 

  • Bouchard M, NH Gosselin, RC Brunet, O Samuel, MJ Dumoulin and G Carrier (2003) A toxicokinetic model of malathion and its metabolites as a tool to assess human exposure and risk through measurements of urinary biomarkers.Toxicol. Sci. 73, 182–194.

    Article  PubMed  CAS  Google Scholar 

  • Brocardo PS, P Pandolfo, RN Takahashi, ALS Rodrigues and AL Dafre (2005) Antioxidant defenses and lipid peroxidation in the cerebral cortex and hippocampus following acute exposure to malathion and/or zinc chloride.Toxicology 207, 283–291.

    Article  PubMed  CAS  Google Scholar 

  • Cabello G, M Valenzuela, A Vilaxa, V Duran, I Rudolph, N Hrepic and G Calaf (2001) A rat mammary tumor model induced by the organophosphorous pesticides parathion and malathion, possibly through acetylcholinesterase inhibition.Environ. Health Perspect. 109, 471–479.

    Article  PubMed  CAS  Google Scholar 

  • Draper HH and M Hadley (1990) Malondialdehyde determination as index of lipid peroxidation.Methods Enzymol. 186, 421–431.

    Article  PubMed  CAS  Google Scholar 

  • Giri S, SB Prasad, A Giri and GD Sharma (2002) Genotoxic effects of malathion: an organophosphorus insecticide, using three mammalian bioassaysin vivo. Mutat. Res. 514, 223–231.

    CAS  Google Scholar 

  • Hazarika A, SN Sarkar, S Hajare, M Kataria, JK Malik (2003) Influence of malathion pretreatment on the toxicity of anilofos in male rats: a biochemical interaction study.Toxicology 185, 1–8.

    Article  PubMed  CAS  Google Scholar 

  • John S, M Kale, N Rathore and D Bhatnagar (2001) Protective effect of vitamin E in dimethoate and malathion induced oxidative stress in rat erythrocytes.J. Nutr. Biochem. 12, 500–504.

    Article  PubMed  CAS  Google Scholar 

  • Johnson VJ, AM Rosenberg, K Lee and BR Blakley (2002) Increased T-lymphocyte dependent antibody production in female S JL/mice following exposure to commercial grade malathion.Toxicology 170, 119–129.

    Article  PubMed  CAS  Google Scholar 

  • Kale M, N Rathore, S John and D Bhatnagar (1999) Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species.Toxicol. Lett. 105, 197–205.

    Article  PubMed  CAS  Google Scholar 

  • Kehrer JP (1993) Free radicals as mediators of tissue injury and disease.Crit. Rev. Toxicol. 34, 21–48.

    Article  Google Scholar 

  • Kock S and U Beisiegel (2000) Lipoproteins in the brain: A new frontier?, In:Lipids and Vascular Disease (Betteridge DJ, Ed) (Martin Dinitz: London), pp 51–64.

    Google Scholar 

  • Koner BC, BD Banerjee and A Ray (1997) Effect ofin vivo generation of oxygen free radicals on immune responsiveness in rabbits.Immunol. Lett. 59, 127–131.

    Article  PubMed  CAS  Google Scholar 

  • Koner BC, BD Banerjee and A Ray (1998) Organochlorine pesticideinduced oxidative stress and immune suppression in rats. IndianJ. Exp. Biol. 36, 395–398.

    CAS  Google Scholar 

  • Liu J and CN Pope (1998) Comparative pre-synaptic neurochemical changes in rat striatum following exposure to chlorpyrifos or parathion.J. Toxicol. Environ. Health Part. A,53, 531–544.

    Article  PubMed  CAS  Google Scholar 

  • Lowry OH, NJ Rosebrough, AL Farr and RJ Randal (1951) Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  • Masoud L, C Vijayasarathy, M Fernandez-Cabezudo, G Petroianu and AM Sale (2003) Effect of malathion on apoptosis of murine L929 fibroblasts: a possible mechanism for toxicity in low dose exposure.Toxicology 185, 89–102.

    Article  PubMed  CAS  Google Scholar 

  • Montine TJ, MD Neely, JF Quinn, MF Beal, WR Markesbery, LJ Roberts II and JD Morrow (2002) Lipid peroxidation in aging brain and Alzheimer’s disease.Free Radic. Biol. Med. 33, 620- 626.

    Article  PubMed  CAS  Google Scholar 

  • Montine TJ, KS Montine, EE Reich, ES Terry, NA Porter and JD Morrow (2003) Antioxidants significantly affect the formation of different classes of isoprostanes and neuroporstanes in rat cerebral synaptosomes.Biochem. Pharmacol. 65, 611–617.

    Article  PubMed  CAS  Google Scholar 

  • Qureshi GA, S Baig, M Sarwar and SH Parvez (2004) Neurotoxicity, oxidative stress and cerebrovascular disorders.NeuroToxicology 25, 121–138.

    Article  PubMed  CAS  Google Scholar 

  • Ranjbar A, P Pasalar and M Abdollahi (2002) Induction of oxidative stress and acetylcholinesterase inhibition in organophosphorus pesticide manufacturing workers.Human Exp. Toxicol. 21, 179- 182.

    Article  CAS  Google Scholar 

  • Rocha JBT, T Emanuelli and ME Pereira (1993) Effects of early undernutrition on kinetic parameters of brain acetylcholinesterase from adults rats.Acta Neurobiol. Exp. 53, 431–437.

    CAS  Google Scholar 

  • Rosenstock L, M Keifer, WE Daniell, R McConnell and K Claypoole (1991) Chronic central nervous system effects of acute organophosphate pesticide intoxication. The Pesticide Health Effects Study Group.Lancet 338, 223–227.

    Article  PubMed  CAS  Google Scholar 

  • Santos HR, WM Cintra, Y Aracava, CM Maciel, NG Castro and EX Albuquerque (2004) Spine density and dendritic branching pattern of hippocampal CA1 pyramidal neurons in neonatal rats chronically exposed to the organophosphate paraoxon.NeuroToxicology 25, 481–494.

    Article  PubMed  CAS  Google Scholar 

  • Shohami E, E Beit-Yannai and M Horowitz (1997) Oxidative stress in closed-head injury.J. Cereb. Blood Flow Metab. 17, 1007–1019.

    Article  PubMed  CAS  Google Scholar 

  • Spiteller G (1996) Enzyme lipid peroxidation a consequence of cell injury.Free Radic. Biol. Med. 21, 1003–1009.

    Article  PubMed  CAS  Google Scholar 

  • Srikanth NS and PK Seth (1990) Alterationinxenobioticmetabolizing enzymes in brain and liver of rats coexposed to endosulfan and malathion.J. Appl. Toxicol. 21, 333–337.

    Google Scholar 

  • Vidella LA, SBM Baros and VBC Junqueira (1990) Lindane induced liver oxidative stress.Free Radic. Biol. Med. 9, 169–179.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratório de Neurociências, Universidade do Extremo Sul Catarinense, 88806-000, Criciúma, SC, Brasil

    JucÉlia J. Fortunato, Gislaine Z. RÉus & JoÃo Quevedo

  2. Laboratório de Fisiopatologia Experimental, Universidade do Extremo Sul Catarinense, 88806-000, Criciúma, SC, Brasil

    Fabiano R. Agostinho, FabrÍcia C. Petronilho & Felipe Dal-Pizzol

Authors
  1. JucÉlia J. Fortunato
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fabiano R. Agostinho
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gislaine Z. RÉus
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. FabrÍcia C. Petronilho
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Felipe Dal-Pizzol
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. JoÃo Quevedo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to JoÃo Quevedo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fortunato, J.J., Agostinho, F.R., RÉus, G.Z. et al. Lipid peroxidative damage on malathion exposure in rats. neurotox res 9, 23–28 (2006). https://doi.org/10.1007/BF03033304

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03033304

Keywords

Search

Navigation