Abstract
Using an Affymetrix GeneChip™ containing 8300 oligonucleotide probes, we measured transcript levels in transgenicArabidopsis overexpressing the jasmonate carboxyl methyltransferase gene (AtJMT). When compared with wild-type plants, 5-week-old transgenics exhibited significant alterations (more than a two-fold increase or decrease) in the expression levels of 168 genes. Among them, 80 were up-regulated, including those involved in defense, oxidative stress-tolerance, and senescence. In contrast, the expression of 88 genes, including those that function in photosynthesis and cold/drought-stress responses, was significantly down-regulated. Thus, endogenous generation of methyl jasmonate through AtjMT-overexpression modified the transcript levels of genes previously identified as being jasmonate-responsive. This result confirms that MeJA formation is a key control point for jasmonate-responsive gene expression in plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Anderson JF;Badruzsaufari E, Schenk PM, Manners JM, Desmond OJ, Ehlert C, Maclean DJ, Ebert PR, Kazan K (2004) Antagonistic interaction between abscisic acid and jasmonate-ethylene signaling pathways modulates defense gene expression and disease resistance inArabidopsis. Plant Cell16: 3460–3479
Barkman TJ (2006) A role for evolutionary predictions in gene isolation and characterization studies. J Piant Biol49: 331–335
Beale MH, Ward JL (1998) Jasmonates: Key players in the plant defense. Nat Prod Rep15: 533–548
Benedetti CE, Arruda P (2002) Altering the expression of the chlorophyllase geneATHCOR1 in transgenic Arabidopsis caused changes in the chlorophyll-to-chlorophyllide ratio. Plant Physiol128: 1255–1263
Benedetti CE, Costa CL, Turcinelli SR, Arruda P (1998) Differential expression of a novel gene in response to coronatine, methyl jasmonate, and wounding in the Coil mutant ofArabidopsis. Piant Physiol116: 1037–1042
Blaszczyk A, Brodzik R, Sirko A (1999) Increased resistance to oxidative stress in transgenic tobacco plants overexpressing bacterial serine acetyltransferase. Plant J20: 237–243
Cheong JJ, Choi YD (2003) Methyl jasmonate as a vital substance in plants. Trends Genet19: 409–413
Cheong YH, Chang HS, Gupta R, Wang X, Zhu T, Luan S (2002) Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses inArabidopsis. Plant Physiol129: 661–677
Cipollini D (2007) Consequences of the overproduction of methyl jasmonate on seed production, tolerance to defoliation and competitive effect and response ofArabidopsis thaliana. New Phytol173: 146–153
Creelman RA, Mullet JE (1997) Biosynthesis and action of jasmonates in plants. Annu Rev Plant Physiol Plant Mol Biol48: 355–381
Cyr DM, Langer T, Douglas MG (1994) DnaJ-like proteins: Molecular chaperones and specific regulators of Hsp70. Trends Biochem Sci19: 176–181
Downie B, Gurusinghe S, Dahai P, Thacker RR, Snyder JC, Nonogaki H, Yim K, Fukanaga K, Alvarado V, Bradford KJ (2003) Expression of aGALATINOL SYNTHASE gene in tomato seeds is up-regulated before maturation desiccation and again after imbibition whenever radicle protrusion is prevented. Plant Physiol131: 1347–1359
Ezaki B, Gardner RC, Ezaki Y, Matsumoto H (2000) Expression of aluminum-induced genes in transgenicArabidopsis plants can ameliorate aluminum stress and/or oxidative stress. Plant Physiol122: 657–665
Farmer EE, Aimeras E, Krishnamurthy V (2003) jasmonates and related oxylipins in plant responses to pathogenesis and herbivory. Curr Opin Plant Biol6: 372–378
Geshi N, Brandt A (1998) Two jasmonate-inducible myrosinase-binding proteins fromBrassica napus L. seedlings with homology to jacalin. Planta204: 295–304
Gfeller A, Farmer EE (2004) Keeping the leaves green above us. Science306: 1515–1516
Howe GA, Schilmiller AL (2002) Oxylipin metabolism in response to stress. Curr Opin Piant Biol5: 230–236
Jung C, Lyou SH, Koo YJ, Song JT, Choi YD, Cheong JJ (2003) Constitutive expression of defense genes in transgenic Arabidopsis overproducing methyl jasmonate. Agric Chem Biotechnol46: 52–57
Jung C, Lyou SH, Yeu SY, Kim MA, Rhee S, Kim M, Lee JS, Choi YD, Cheong JJ (2007) Microarray-based screening of jasmonate-responsive genes inArabidopsis thaliana. Plant Cell Rep, in press
Kawaoka A, Kaothien P, Yoshida K, Endo S, Yamada K, Ebinuma H (2000) Functional analysis of tobacco LIM protein Ntlim1 involved in lignin biosynthesis. Plant J22: 289–301
Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K (1994) Characterization of two cDNAs (ERD10 andERD14) corresponding to genes that respond rapidly to dehydration stress inArabidopsis thaliana. Plant Cell Physiol35: 225–231
Miller JD, Arteca RN, Pell EJ (1999) Senescence-associated gene expression during ozone-induced leaf senescence inArabidopsis. Plant Physiol120: 1015–1024
Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci7: 405–410
Schenk PM, Kazan K, Rusu AG, Manners JM, Maclean DJ (2005) TheSEN1 geneof Arabidopsis is regulated by signals that link plant defence responses and senescence. Plant Physiol Biochem43: 997–1005
Seo HS, Song JT, Cheong JJ, Lee YH, Lee YW, Hwang I, Lee JS, Choi YD (2001) Jasmonic acid carboxyl methyltransferase: A key enzyme for jasmonate-regulated plant responses. Proc Natl Acad Sci USA98: 4788–4793
Song JT, Seo HS, Song SI, Lee JS, Choi YD (2000)NTR1 encodes a floral nectary-specific gene inBrassica campestris L. ssp.pekinensis. Plant Mol Biol42: 647–655
Song MS, Kim DG, Lee SH (2005) Isolation and characterization of a jasmonic acid carboxyl methyltransferase gene from hot pepper (Capsicum annuum L.). J Plant Biol48: 292–297
Taipalensuu J, Eriksson S, Rask L (1997) The myrosinase-binding protein fromBrassica napus seeds possesses lectin activity and has a highly similar vegetatively expressed wound-inducible counterpart. Eur J Biochem250: 680–688
Wang H, Datla R, Georges F, Loewen M, Cutler AJ (1995) Promoters fromkin1 andcor6.6, two homologousArabidopsis thaliana genes: Transcriptional regulation and gene expression induced by low temperature, ABA, osmoticum and dehydration. Plant Mol Biol28: 605–617
Wasternack C, Hause B (2002) Jasmonates and octadecanoids: Signals in plant stress responses and development. Progr Nucl Acid Res Mol Biol72: 165–221
Xiong L, Schumaker KS, Zhu JK (2002) Cell signaling during cold, drought, and salt stress. Plant Cell14: S165-S183
Zhu T, Budworth P, Han B, Brown D, Chang HS, Zou G, Wang X (2001) Toward elucidating the global gene expression patterns of developingArabidopsis: Parallel analysis of 8300 genes by a high-density oligonucleotide probe array. Plant Physiol Biochem 39: 221–242
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jung, C., Yeu, S.Y., Koo, Y.J. et al. Transcript profile of transgenicArabidopsis constitutively producing methyl jasmonate. J. Plant Biol. 50, 12–17 (2007). https://doi.org/10.1007/BF03030594
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03030594