Abstract
In order to find potent virus-cell fusion inhibitory components from Korean edible clams, thirteen prepared polysaccharides were introduced to syncytia formation inhibition assay, which is based on the interaction between the HIV-1 envelope protein gp120/41 and the cellular membrane protein CD4 of T lymphocytes. Among them,Meretrix petechialis showed a potent viruscell fusion inhibitory activity. Fusion index (FI) and percent (%) fusion inhibition of the polysaccharide of this clam were 0.21 ±0.02, and 67.52±4.09 at 100μg/ml, respectively. It exhibited almost equivalent virus-cell fusion inhibitory activity to that of dextran sulfate which was used as a standard control.
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Amornrut, C., Toida, T., Imanari, T., Woo, E. -R., Park, H., Linhardt, R., Wu, S. J., Kim, Y. S., A new sulfated β-galactan from clams with anti-HIV activity.Carbohydr. Res. 32, 121–7 (1999).
Cheserbo, B., and Wehrly, K., Development of a sensitive quantitative focal assay for human immunodeficiency virus infectivity.J. Virol., 62, 3779–3788 (1988).
De Clercq, E., Suramin in the treatment of AIDS: mechanism of action.Antiviral Res., 7, 1–10 (1987).
De Clercq, E., HIV resistance to reverse transcriptase inhibitors.Biochem. Pharmacol., 47, 155–169 (1994).
De Clercq, E., Antiviral therapy for human immunodeficiency virus infections.Clin. Microbiol. Rev., 8, 200–239 (1995).
Earl, P. L., Hugin, A. W., and Moss, B., Removal of cryptic poxvirus transcription termination signals from the human immunodeficiency virus type 1 envelope gene enhances expression and immunogenicity of a recombinant vaccinia virus.J. Virol., 64, 2448–2451 (1990).
Faulds, D., and Brogden, R. N., Didanosine. a review of its antiviral activity, pharmacokinetic properties and therapeutic potential in human immunodeficiency virus infection.Drugs, 44, 94–116 (1992).
Hart, T. K., Kirsh, R., Ellens, H., Sweet, R. W., Lambert, D. M., Petteway, S. R., Leary, J., and Bugelski, P. J., Binding of soluble CD4 proteins to human immunodeficiency virus type 1 and infected cells induces release of envelope glycoprotein gp120.Proc. Natl. Acad. Sci. USA, 88, 2189–2193 (1991).
Kim, Y. S., Jo, Y. Y., Chang, I. -M., Toida, T., Park, Y. M. Linhardt, R. J., A New Glycosaminoglycan from the Giant African Snail fromAchatina fulica.J. Biol. Chem., 271, 1750–11755 (1996).
Kim, H. J., Woo, E. -R., Shin, C. -G., and Park, H., A new flavonol glycoside gallate fromAcer okamotoanum and its inhibitory activity against human immunodeficiency virus (HIV) integrase.J. Nat. Prod., 61, 145–148 (1998).
Kim, H. J., Lee, J. S., Woo, E. -R., Kim, M. K., Yang, B. S., Yu, Y. G., Park, H., and Lee, Y. S., Isolation of virus-cell fusion inhibitory components fromEugenia caryophyllata.Planta med., 67, 99–102 (2001).
Langtry, H. D., and Campoli-Richards, D. M., Zidobudine, a review of its pharmacodynamic and pharmacokinetic properities, and therapeutic efficacy.Drugs, 37, 408–450 (1989).
Lin, P. -F., Samanta, H., Bechtold, C. M., Deminie, C. A., Patick, A. K., Alam, M., Riccardi, K., Rose, R. E., White, R. J., and Colonno, R. J., Characterization of siamycin l, a human immunodeficiency virus fusion inhibitor.Antimicrob.Agents Chemother., 40, 133–138 (1996).
Mayaux, J. -F., Bousseau, A., Pauwels, R., Huet, T., Henin, Y., Dereu, N., Evers, M., Soler, F., Poujade, C., De Clercq, E., and Le Pecq, J. -B., Triterpene derivatives that block entry of human immunodeficiency virus type 1 into cells.Proc. Natl. Acad. Sci. USA, 91, 3564–3568 (1994).
Nakashima, H., Ichiyama, K., Inazawa, K., Ito, M., Hayashi, H., Nishihara, Y., Tsujii, E., and Kino, T., FR901724, a novel anti-human immunodeficiency virus (HIV) peptide produced by Streptomyces, shows synergistic antiviral activities with HIV protease inhibitor and 2, 3-dideoxy-nucleosides.Biol. Pharm. Bull., 19, 405–412 (1996).
Pereira, F. B., Goni, F. M., and Nieva, J. L., Liposome dest-abilization induced by the HIV-1 fusion peptide effect of a single amino acid substitution.FEBS Letters, 362, 243–246 (1995).
Ryu, J. -R., Jin, B. -S., Suh, M. -J., Yoo, Y. -S., Yoon, S. H., Woo, E. -R., and Yu, Y. G., Two interaction modes of the gp41 -derived peptides with gp41 and their correlation with antimembrane fusion activity.Biochem. Biophys. Res. Commun., 265, 625–629 (1999).
Ryu, J. -R., Lee, J., Choo, S., Yoon, S. H., Woo, E. -R., and Yu, Y. G., Development of an in vitro assay system for screening of gp 41 inhibitory compounds.Mol. Cells., 8, 717–723 (1998).
Tochikura, T. S., Nakashima, H., Tanabe, A., Yamamoto, N., Human immunodeficiency virus (HlV)-induced cell fusion: Quantification and its application for the simple and rapid screening of anti-HIV substances in vitro.Virol., 164, 542–546 (1988).
Whittington, R., and Brogden, R. N., Zalcitabine, a review of its pharmacology and clinical potential in acquired immunodeficiency syndrome (AIDS).Drugs, 44, 656–683 (1992).
Woo, E. -R., Yoon, S. H., Kwak, J. H., Kim, H. J., and Park, H., Inhibition of gp120-CD4 interaction by various plant extracts.Phytomedicine, 4, 53–58 (1997).
Wu, S. J., Chun, M. W., Shin, K. H., Toida, T., Park, Y., Linhardt, R. J., and Kim, Y. S., Chemical oversulfation and anticoagulant activity of acharan sulfate.Thromb. Res., 92, 273–281 (1998).
Yao, X-J., Wainberg, M. A., and Parniak, M. A., Mechanism of inhibition of HIV-1 in vitro by purified extracts ofPrunella vulgaris.Virol., 187, 56–62 (1992).
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Woo, ER., Kim, W.S. & Kim, Y.S. Virus-cell fusion inhibitory activity for the polysaccharides from various Korean edible clams. Arch Pharm Res 24, 514–517 (2001). https://doi.org/10.1007/BF02975155
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DOI: https://doi.org/10.1007/BF02975155