Abstract
Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)], by fed-batch culture of recombinantEscherichia coli harboring a plasmid containing theAlcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only. In a 30 L fermentor having aK La value of 0.11 s−1, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively, giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having aK La of 0.03 s−1, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively, giving a productivity of 1.06 g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinantE. coli in a large-scale fermentor having lowK La value.
Similar content being viewed by others
References
Lee, S. Y. (1996) Bacterial polyhydroxyalkanoates,Biotechnol. Bioeng. 49: 1–14.
Steinbüchel, A. and B. Füchtenbusch (1998) Bacterial and other biological systems for polyester production.Trends Biotechnol. 16: 419–427.
Lee, S. Y. (1996) Plastic bacteria? Progress and prospects for polyhydroxyalkanoate production in bacteria.Trends Biotechnol. 14: 431–438.
Choi, J. and S. Y. Lee (1997) Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation.Bioprocess Eng. 17: 335–342.
Choi, J., S. Y. Lee, and K. Han (1998) Cloning of theAlcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of the these genes for enhanced production of poly(3-hydroxybutyrate) by recombinantEscherichia coli.Appl. Environ. Microbiol. 64: 4897–4903.
Choi, J. and S. Y. Lee (1999) High level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fedbatch culture of recombinantEscherichia coli.Appl. Environ. Microbiol. 65: 4363–4368.
Braunegg, G., B. Sonnleitner, and R. M. Lafferty (1978) A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass.Eur. J. Appl. Microbiol. Biotechnol. 6: 29–37.
Chang, H. N., B. Halard, and M.-Y. Murray (1989) Measurement ofK La by a gassing-in method with oxygenenriched air.Biotechnol. Bioeng. 34: 1147–1157.
Lee, S. Y. and S. J. Park (2002) Fermentative production of SCL-PHAs. pp. 262–290. In: Y. Doi and A. Steinbüchel (eds.)Biopolymers. Vol. 3a. Wiley-VCH, Germany.
Lee, S. Y. and S. J. Park (2002) Biosynthesis and fermentative production of SCL-MCL-PHAs, pp. 317–336, In: Y. Doi and A. Steinbüchel (eds.).Biopolymers, Vol. 3a. Wiley-VCH, Germany.
Mehta, V. D. and M. M. Sharma (1971) Mass transfer in mechanically agitated gas-liquid contactors.Chem. Eng. Sci. 26: 461–479.
van Wegen, R. J., S. Y. Lee and A. P. J. Middelberg (2001) Metabolic and kinetic analysis of poly(3-hydroxybutyrate) production by recombinantEscherichia coli.Biotechnol. Bioeng. 74: 70–80.
Wong, H. H., R. J. van Wegen, J. Choi, S. Y. Lee, and A. P. J. Middelberg (1999) Metabolic analysis of poly(3-hydroxybutyrate) production by recombinantEscherichia coli.J. Microbiol. Biotechnol. 9: 593–603.
Park, S. J., J. P. Park, and S. Y. Lee (2002) Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinantEscherichia coli in a pilot-scale fermenter.Biotechnol. Lett. 24: 185–189.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Choi, Ji., Lee, S.Y., Shin, K. et al. Pilot scale production of poly(3-hydroxybutyrate-co-3-hydroxy-valerate) by fed-batch culture of recombinantEscherichia coli . Biotechnol. Bioprocess Eng. 7, 371–374 (2002). https://doi.org/10.1007/BF02933524
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02933524