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Comparative studies of differential expression of chitinolytic enzymes encoded bychiA, chiB, chiC andnagA genes inAspergillus nidulans

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Abstract

N-Acetyl-d-glucosamine, chito-oligomers and carbon starvation regulatedchiA, chiB, andnagA gene expressions inAspergillus nidulans cultures. The gene expression patterns of the main extracellular endochitinase ChiB and theN-acetyl-β-d-glucosaminidase NagA were similar, and the ChiB-NagA enzyme system may play a morphological and/or nutritional role during autolysis. Alterations in the levels of reactive oxygen species or in the glutathione-glutathione disulfide redox balance, characteristic physiological changes developing in ageing and autolyzing fungal cultures, did not affect the regulation of either the growth-relatedchiA or the autolysis-coupledchiB genes although both of them were down-regulated under diamide stress. The transcription of thechiC gene with unknown physiological function was repressed by increased intracellular superoxide concentration.

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Abbreviations

CBD:

chitin-binding domain

CV:

coefficient of variance

DCM:

dry cell mass

GlcNAc:

N-acetyl-d-glucosamine

(GlcNAc)2 :

N,N′-diacetylchitobiose

(GlcNAc)3 :

N,N′,N″-triacetylchitotriose

GlcNAcase:

β-N-acetylhexosaminidase (N-acetyl-β-d-glucosaminidase; EC 3.2.1.52)

GSH:

glutathione

GSSG:

glutathione disulfide

MSB:

2-methyl-1,4-naphthoquinone sodium hydrogen sulfite (‘menadione sodium bisulfite’)

NCBI:

National Center for Biotechnology Information

4NPGlcNAc:

4-nitrophenylN-acetyl-β-d-glucosaminide

4NP(GlcNAc)2 :

4-nitrophenylN,N′-diacetyl-β-d-chitobioside

4NP(GlcNAc)3 :

4-nitrophenylN,N′,N″-triacetyl-β-d-chitotrioside

ORF:

open reading frame

PMF:

peptide mass fingerprint

PSD:

post-source decay

ROS:

reactive oxygen species

RT-PCR:

real-time polymerase chain reaction

SD:

standard deviation

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. Department of Microbial Biotechnology and Cell Biology, University of Debrecen, 4010, Debrecen, Hungary

    T. Pusztahelyi, Z. Molnár, T. Emri, M. Miskei & I. Pócsi

  2. Department of Biochemistry, Faculty of Science, University of Debrecen, 4010, Debrecen, Hungary

    J. Kerékgyártó

  3. Protecomics Research Group, Biological Research Center, 6726, Szeged, Hungary

    É. Klement

  4. Department of Nephrology, Faculty of Medicine, University of Debrecen, 4012, Debrecen, Hungary

    J. Balla

Authors
  1. T. Pusztahelyi
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  2. Z. Molnár
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  3. T. Emri
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  4. É. Klement
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  5. M. Miskei
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  6. J. Kerékgyártó
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  7. J. Balla
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  8. I. Pócsi
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Corresponding author

Correspondence to I. Pócsi.

Additional information

The first author was a recipient of aHungarian Scientific Research Fund postdoctoral fellowship (OTKA grant no. D034568); the third author was a grantee of theBolyai János Scholarship. TheHungarian Ministry of Education awarded aSzéchenyi Scholarship for Professors to the last author.

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Pusztahelyi, T., Molnár, Z., Emri, T. et al. Comparative studies of differential expression of chitinolytic enzymes encoded bychiA, chiB, chiC andnagA genes inAspergillus nidulans . Folia Microbiol 51, 547–554 (2006). https://doi.org/10.1007/BF02931619

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  • DOI: https://doi.org/10.1007/BF02931619

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