Abstract
For clonal propagation ofAsparagus cooperi, shoot tips and node explants of 7, 20 and 35 d old spear from the region within 10 cm and below 25 cm from the apex were cultured in modified Murashige and Skoog's (1962) medium containing 6-benzylaminopurine (BAP). The required concentration of BAP varied in explants of different ages and types. In shoot tip culture, the rate of shoot multiplication was higher after 40 d than 60 d of culture. The maximum mumber (62–65) of shoots were obtained from shoot tip explants of 20 d old spear in the medium containing 2.0 mg dm−3 of BAP, 80 mg dm−3 of adenine and 0.02 mg dm−3 of α-naphthalene acetic acid after 60 d of culture. From node cultures, high number of shoots were obtained after 30 d. Pretreatment with BAP in liquid medium for 48 h was effective for semirejuvenescence. The individual shoots produced roots in presence of indole-3-butyric acid and also in potassium salt of indole-3-butyric acid, the later being more effective. All regeneratns were cytologically stable.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- IBA:
-
indole-3-butyric acid
- KIBA:
-
potassium salt of indole-3-butyric acid
- NAA:
-
α-naphthalene acetic acid
- Ade:
-
adenine
- 2ip:
-
N6-(2-isopentenyl)-adenine
- Kin:
-
kinetin
- MS medium:
-
medium of Murashige and Skoog (1962).
References
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Ghosh, B., Sen, S. Micropropagation ofAsparagus cooperi as affected by growth regulators. Biol Plant 36, 527–534 (1994). https://doi.org/10.1007/BF02921173
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DOI: https://doi.org/10.1007/BF02921173