Abstract
α-Acetolactate decarboxylase has been purified to homogeneity, by fast protein liquid chromatography and high performance elution chromatography, from a partially purified α-acetolactate decarboxylase preparation from Lactobacillus casei DSM 2547. The pure enzyme exhibited a specific activity of 375 kU·mg−1 and exerted its optimal activity at pH 4.5 to 5.0 and at a temperature of 40°C. Its isoelectric point was estimated to pH 4.7 and its molecular weight was found to be 48,000. The enzyme was inhibited by o-phenanthroline and could be partially reactivated by zinc ions. An HPLC method for the determination of α-acetolactate is described.
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Abbreviations
- ALDC:
-
α-acetolactate decarboxylase
- AUFS:
-
absorbance units full scale
- BSA:
-
bovine serum albumin
- DSM:
-
Deutsche Sammlung von Mikroorganismen
- EDTA:
-
ethylene diaminetetraacetic acid
- HPEC:
-
high performance exclusion chromatography
- HPLC:
-
high performance liquid chromatography
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Accepted by:H. Klenow, E. Lund andS.O. Andersen
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Rasmussen, A.M., Gibson, R.M., Godtfredsen, S.E. et al. Purification of α-acetolactate decarboxylase from Lactobacillus casei DSM 2547. Carlsberg Res. Commun. 50, 73 (1985). https://doi.org/10.1007/BF02907138
DOI: https://doi.org/10.1007/BF02907138