Abstract
Exon trapping from cosmids mapping to chromosome 19q13.3 yielded 6 exonic sequences that matched the human symplekin gene, which encodes a tight junction-related protein. One exonic sequence identified a 4.0 kb brain cDNA clone, R6E1, which contained 302 bp 5′ to the originally reported 3.7 kb symplekin cDNA. A portion of this novel 5′ sequence matched an additional trapped exonic sequence which was obtained from the most telomeric cosmid analyzed. The symplekin gene thus lies in a telomeric-to-centromeric direction on 19q13.3. Only three cosmids from a large 19q13.3 contig hybridized with R6E1, thereby assigning the symplekin gene to a 40 kb region immediately telomeric to gene 59 and the DM protein kinase gene. The 5′ end of the R6E1 clone has a potential initiation codon with a strong Kozak sequence and Northern blot analysis detected a 4.2 kb signal in most human tissues, indicating that R6E1 may be a complete cDNA sequence. Based on the trapped exonic sequences, twelve exon-intron boundaries were predicted.
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The sequence data in this paper have been deposited in GenBank, accession number U88726.
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Ueki, K., Ramaswamy, S., Billings, S.J. et al. Chromosomal localization to 19q13.3, partial genomic structure and 5′ cDNA sequence of the human symplekin gene. Somat Cell Mol Genet 23, 229–231 (1997). https://doi.org/10.1007/BF02721375
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DOI: https://doi.org/10.1007/BF02721375