Summary
In order to evaluate the intersample variations for bone histomorphometric parameters in various metabolic bone diseases, either for a group or for one single patient, two complete contiguous transiliac bone biopsies were taken in 55 subjects. The diagnoses were osteoporosis (OP), renal osteodystrophy (ROD), osteomalacia (OM), primary hyperparathyroidism (HPT), metastatic bone disease, fluorosis, thyrotoxic bone, and “normal” bone. The following histomorphometric parameters were measured: trabecular bone volume (TBV), trabecular osteoid surfaces (TOS) and volume (TOV), trabecular resorption surfaces (TRS), and calcification rate (CR). The thickness index of osteoid seams (TIOS) was calculated. The measurements were performed with both manual and computerized methods which give similar results according to our previous study. The differences between parameters values measured on both cores were expressed by the difference in percent of the mean and by the intrapair coefficient of variation. Moreover, for each parameter, the confidence interval for one subject was calculated from the residual mean square of a two-way analysis of variance. For each parameter, the intersample variation differs according to the diagnosis. Confidence interval (risk=5%) for one single subject reaches 29% for TBV in OP; 16% for TOS; 26% for TOV and TIOS in OM; 25% for TRS in ROD, and 69% in HPT, but is much lower for groups of 10 and 20 patients. These variations must be taken into account when successive biopsies are performed in one individual or in groups of patients to follow the course of a disease or evaluate the effects of a therapy.
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References
Garner A, Ball J (1966) Quantitative observations on mineralised and unmineralised bone in chronic renal azotemia and intestinal malabsorption syndrome. J Pathol Bact 91:545–561
Ellis HA, Peart KM (1972) Quantitative observations on mineralized and non-mineralized bone in the iliac crest. J Clin Pathol 25:277–286
Garrick R, Doman P, Posen S (1972) Quantitative histology of bone: the use of a computer program and results in normal subjects. Clin Sci 43:789–797
Meunier PJ, Courpron P (1973) Iliac trabecular bone volume in 236 controls. Representativeness of iliac samples. In: Jaworski ZFG (ed) Proceeding of the First Workshop on Bone Morphometry. University of Ottawa Press, Ottawa, p 100
Courpron P, Meunier PJ, Bressot C, Giroux JM (1976) Amount of bone in iliac crest biopsy. Significance of the trabecular bone volume: its values in normal and in pathological conditions. In: Meunier PJ (ed) Bone histomorphometry. Second International Workshop, Armour Montagu, Paris, Lyon, p 39
Visser WJ, Niermans HJ, Roelofs JMM, Raymakers JA, Duursma SA (1976) Comparative morphometry of bone biopsies obtained by two different methods from the right and the left iliac crest. In: Meunier PJ (ed) Bone histomorphometry. Second International Workshop, Armour Montagu, Paris, Lyon, p 79
Eisler P, Reich TH, Binswanger U (1977) Zur Morphometric von Beckenkammbiopsien. Microsc Acta 79 (5):393–414
Bergot C, Laval-Jeantet AM, Laval-Jeantet M (1978) Etude critique des variations de la mesure de la masse osseuse par biopsie iliaque. Rev Rhum 45 (5):317–324
Melsen F, Melsen B, Mosekilde L (1978) An evaluation of the quantitative parameters applied in bone histology. Acta Pathol Microbiol Scand Sect A 86:63–69
Delling G, Luehmann H, Baron R, Mathews CHE, Olah A (1980) Investigation of intra- and inter-reader reproductibility. Metab Bone Dis Rel Res Vol 2 S:419–427
Vernejoul MC, Kuntz D, Miravet L, Goutallier D, Ryckewaert A (1981) Bone histomorphometry reproductibility in normal patients. Calcif Tissue Int 33:369–374
Visser WJ, Roelofs JMM, Duursma SA (1981) Bone density in the iliac crest. Metab Bone Dis Rel Res 3:187–190
Malluche HH, Meyer W, Sherman D, Massry SG (1982) Quantitative bone histology in 84 normal American subjects. Micromorphometric analysis and evaluation of variance in iliac bone. Calcif Tissue Int 34:449–455
Lo Cascio V, Bonucci E, Imbimbo B, Ballanti P, Tartarotti D, Galvanini G, Fucella L, Adami S (1984) Bone loss after glucocorticoid therapy. Calcif Tissue Int 36:435–438
Meunier PJ, Edouard C, Richard D, Laurent J (1976) Histomorphometry of osteoid tissue: the hyperosteoidoses. In: Meunier PJ (ed) Bone histomorphometry. Second International Workshop. Armour Montagu, Paris, Lyon, p 69
Malluche H, Ritz E, Lange HP, Arras D, Schoeppe W (1976) Bone mass in maintenance hemodialysis. Prospective study with sequential biopsies. J Clin Invest 6:265–271
Vernejoul MC, Kuntz D, Miravet L, Gueris J, Blelakoff J, Ryckewaert A (1981) Bone histomorphometry in hemodialyzed patients. Metab Bone Dis Rel Res 3:175–179
Frost HM (1969) Tetracycline-based histological analysis of bone remodeling. Calcif Tissue Res 3:211–237
Meunier PJ (1980) Bone biopsy in diagnosis of metabolic bone disease. In: Cohn DV, Talmage RV, Les Matthews J (eds) Hormonal control of calcium metabolism. Proceedings of the Seventh International Conference on Calcium Regulating Hormones, Excerpta Medica, Colorado, p 109
Meunier PJ, Courpron P, Edouard C, Bernard J, Bringuier J, Vignon G (1973) Physiological senile involution and pathological rarefaction of bone. Quantitative and comparative histological data. Clinics in Endocrinol and Metab 2:239–256
Meunier PJ, Edouard C, Courpron P, Toussaint F (1975) Morphometric analysis of osteoid in iliac trabecular bone. Methodology. Dynamical significance of the osteoid parameters. In: Norman AW, Schaeffer K, Grigoleit HG, Von Herrath D, Ritz E (eds) Walter de Gruyter Berlin, p 149
Vedi S, Compston JE (1984) Direct and indirect measurement of osteoid seam width in human iliac crest trabecular bone. Metab Bone Dis Rel Res 5:269–274
Schwartz D (ed) (1963) Méthodes statistiques à l'usage des médecins et des biologistes. Flammarion Médecine Sciences, Paris, p 135
Dagnelie P (ed) (1975) Théorie et méthodes statistiques. Vol. 2. Les Presses Agronomiques de Gembloux. Gembloux. Belgium, p 153
Chavassieux PM, Arlot ME, Meunier PJ (in press) Intermethod variation in bone histomorphometry. Comparison between manual and computerized methods applied to iliac bone biopsies. Metab Bone Dis Rel Res
Whitehouse WJ (1974) The quantitative morphology of anisotropic trabecular bone. J Microsc 101:153–168
Sherrard DJ, Ott S, Malloney N, Andress D, Coburn J (1983) Uremic osteodystrophy: Classification, cause and treatment. In: Frame B, Potts J (eds) Clinical disorders of bone and mineral metabolism. Excerpta Medica, Amsterdam, p 254
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Chavassieux, P.M., Arlot, M.E. & Meunier, P.J. Intersample variation in bone histomorphometry: Comparison between parameter values measured on two contiguous transiliac bone biopsies. Calcif Tissue Int 37, 345–350 (1985). https://doi.org/10.1007/BF02553699
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DOI: https://doi.org/10.1007/BF02553699