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Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma

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Abstract

Background: The aim of this study was to investigate the prognostic value of cell proliferation (Ki-67 antigen) and DNA content in patients resected for distal bile duct carcinoma (DBDC).

Methods: Formalin-fixed tumor specimens of 35 patients with resected DBDC and a long-term clinical follow-up were analyzed. MIB-1 antibody was used for Ki-67 antigen detection to determine the proportion of proliferating cells. DNA content was measured using flow cytometry.

Results: A significant correlation was found between a low MIB-1 index (<20%) and survival (P<.05). Of the 35 tumor specimens, 34 specimens were evaluable by flow cytometry: 22 carcinomas were diploid (65%), and 12 were aneuploid (35%). The median DNA index of aneuploid tumors was 1.36 (range, 1.09 to 1.76). No correlation of DNA-ploidy with survival time was found.

Conclusion: In contrast to DNA-ploidy pattern, Ki-67 antigen expression showed prognostic significance in resectable DBDC. A Ki-67 positive ratio of ⩾20% was associated with decreased survival time.

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Authors and Affiliations

  1. the Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

    Arjen M. Rijken MD, Anne Bosma MD, Mirjam M. Polak BA & G. Johan A. Offerhaus MD

  2. Dept. of Surgery, Academic Medical Center, University of Amsterdam, 9 Meibergdreef, 1105 AZ, Amsterdam, The Netherlands

    Arjen M. Rijken MD, Akiko Umezawa MD, Thomas M. van Gulik MD, Huug Obertop MD & Dirk J. Gouma MD

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  1. Arjen M. Rijken MD
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  2. Akiko Umezawa MD
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  3. Thomas M. van Gulik MD
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  4. Anne Bosma MD
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Rijken, A.M., Umezawa, A., van Gulik, T.M. et al. Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma. Annals of Surgical Oncology 5, 699–705 (1998). https://doi.org/10.1007/BF02303480

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  • DOI: https://doi.org/10.1007/BF02303480

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