Summary
Mitochondrial Complex I deficiencies have been described not only in the brain but also in the skeletal muscle and platelets in Parkinson's disease (PD). We report activities of Complex I, II, III, and IV in lymphocytes and platelets in 20 patients with PD and age-matched controls. A small but a significant decrease in the platelet Complex I activity was found in PD (9.14±1.86 units/mg protein) compared with that in the control (12.37±2.66 units/mg protein) (P=0.0002). The lymphocyte Complex I activity was about the same between PD and the control. The activity of Complex II was slightly diminished in both platelets and lymphocytes in PD. Rather small decrease in the platelet Complex I activity in PD may be clinically non-significant. But it may indicate the presence of a qualitatively similar abnormality in platelets as in the nigro-striatal neurons. The cause for decrease in the Complex II activity is unknown at this moment. Further studies seem necessary.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bindoff LA, Birch-Machin M, Cartlidge NEF, Parker WD Jr, Turnbull DM (1989) Mitochondrial function in Parkinson's disease. Lancet i: 49
DiMonte D, Jewell SA, Ekstrom G, Sandy MS, Smith MT (1986) 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridine (MPP+) cause rapid ATP depletion is isolated hepatocytes. Biochem Biophys Res Commun 137: 310–315
Heikkila RE, Nicklas WJ, Vyas I, Duvoisin RC (1985) Dopaminergic toxicity of rotenone and the 1-methyl-4-phenylpyridinium ion after their stereotaxic administration to rats: implication for the mechanism of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine toxicity. Neurosci Lett 62: 389–394
Ikebe S, Tanaka M, Ohno K, Sato W, Hattori K, Kondo T, Mizuno Y, Ozawa T (1990) Increase of deleted mitochondrial DNA in the striatum in Parkinson's disease and senescence. Biochem Biophys Res Commun 170: 1044–1048
Linnane AW, Marzuki S, Ozawa T, Tanaka M (1989) Mitochondrial DNA mutations as an important contributor to ageing and degenerative diseases. Lancet i: 642–645
Mizuno Y, Sone N, Saitoh T (1986) Dopaminergic neurotoxins, MPTP and MPP+, inhibit activity of mitochondrial NADH-ubiquinone oxidoreductase. Proc Jpn Acad Ser B 62: 261–263
Mizuno Y, Saitoh T, Sone N (1987a) Inhibition of mitochondrial NADH-ubiquinone oxidoreductase activity by 1-methyl-4-phenylpyridinium ion. Biochem Biophys Res Commun 143: 294–299
Mizuno Y, Saitoh T, Sone N (1987b) Inhibition of mitochondrial alpha-ketoglutarate dehydrogenase by 1-methyl-4-phenylpyridinium ion. Biochem Biophys Res Commun 143: 971–976
Mizuno Y, Sone N, Saitoh T (1987c) Effects of 1-methyl-4-phenyl-1,2,3,5-tetrahydropyridine and 1-methyl-4-phenylpyridinium ion on activities of the enzymes in the electron transport system in mouse brain. J Neurochem 48: 1787–1793
Mizuno Y, Suzuki K, Sone N, Saitoh T (1987d) Inhibition of ATP synthesis by 1-methyl-4-phenylpyridinium ion (MPP+) in isolated mitochondria from mouse brain. Neurosci Lett 81:204–208
Mizuno Y, Sone N, Suzuki K, Saitoh T (1988a) Studies on the toxicity of 1-methyl-4-phenylpyridinium ion (MPP+) against mitochondria of mouse brain. J Neurol Sci 86: 97–110
Mizuno Y, Suzuki K, Sone N, Saitoh T (1988b) Inhibition of mitochondrial respiration by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in mouse brain in vivo. Neurosci Lett 91: 349–353
Mizuno Y, Ohta S, Tanaka M, Takamiya S, Suzuki K, Sato T, Oya H, Ozawa T, Kagawa Y (1989) Deficiencies in Complex I subunits of the respiratory chain in Parkinson's disease. Biochem Biophys Res Commun 163: 1450–1455
Mizuno Y, Suzuki K, Ohta S (1990) Postmortem changes in mitochondrial respiratory enzymes in brain and a preliminary observation in Parkinson's disease. J Neurol Sci 96: 49–57
Nagatsu T, Yoshida M (1988) An endogenous substance of the brain, tetrahydroisoquinoline, produces parkinsonism in primates with decreased dopamine, tyrosine hydroxylase and biopterin in the nigrostriatal regions. Neurosci Lett 87: 178–182
Naoi M, Matsuura S, Parvez H, Takahashi T, Hirata Y, Minami M, Nagatsu T (1989) Oxidation of N-methyl-1,2,3,4-tetrahydroisoquinoline into N-methyl-isoquinolinium ion by monoamine oxidase. J Neurochem 52:653–655
Nicklas WJ, Vyas I, Heikkila RE (1985) Inhibition of NADH-linked oxidation in brain mitochondria by 1-methyl-4-phenylpyridine, a metabolite of the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Life Sci 36: 2503–2508
Niwa T, Takeda N, Kaneda N, Hashizume Y, Nagatsu T (1987) Presence of tetrahydroisoquinoline and 1-methyl-tetrahydroisoquinoline in parkinsonian and normal human brains. Biochem Biophys Res Commun 144: 1084–1089
Parkash L (1975) Repair of pyrimidine dimers in nuclear and mitochondrial DNA of yeast irradiated with low doses of ultraviolet light. J Mol Biol 98:781–795
Parker WD, Boyson SJ, Parks JK (1989) Abnormalities of the electron transport chain in idiopathic Parkinson's disease. Ann Neurol 26: 719–723
Ramsay RR, Salach JI, Singer TP (1986a) Uptake of the neurotoxin 1-methyl-4-phenylpyridine (MPP+) by mitochondria and its relation to the inhibition of the mitochondrial oxidation of NAD+-linked substrates by MPP+. Biochem Biophys Res Commun 134: 743–748
Ramsay RR, Salach JI, Dadgar J, Singer TP (1986b) Inhibition of mitochondrial NADH dehydrogenase by pyridine derivatives and its possible relation to experimental and idiopathic Parkinsonism. Biochem Biophys Res Commun 135: 269–275
Ramsay RR, Singer TP (1986c) Energy dependent uptake of N-methyl-4-phenyl-pyridinium, the neurotoxic metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, by mitochondria. J Biol Chem 261: 7585–7587
Richter C, Park JW, Ames BN (1988) Normal oxidative damage to mitochondrial and nuclear DNA is extensive. Proc Natl Acad Sci USA 85: 6465–6467
Schapira AHV, Cooper JM, Dexter D, Jenner P, Clark JB, Marsden CD (1989) Mitochondrial complex I deficiency in Parkinson's disease. Lancet i: 1269
Schapira AHV, Cooper JM, Dexter D, Clark JM, Jenner P, Marsden CD (1990) Mitochondrial complex I deficiency in Parkinson's disease. J Neurochem 54: 823–827
Suzuki K, Mizuno Y, Yoshida M (1989) Selective inhibition of Complex I of the brain electron transport system by tetrahydroisoquinoline. Biochem Biophys Res Commun 162: 1541–1545
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yoshino, H., Nakagawa-Hattori, Y., Kondo, T. et al. Mitochondrial complex I and II activities of lymphocytes and platelets in Parkinson's disease. J Neural Transm Gen Sect 4, 27–34 (1992). https://doi.org/10.1007/BF02257619
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02257619