Abstract
The complete mitochondrial DNA (mtDNA) molecules ofHomo and of the common chimpanzee were sequenced. Each sequence was established from tissue of one individual and thus nonchimeric. Both sequences were assembled in their entirety from natural (not PCR amplified) clones. Comparison with sequences in the literature identified the chimpanzee specimen asPan troglodytes verus, the West African variety of the species. The nucleotide difference between the complete human and chimpanzee sequences is 8.9%. The difference between the control regions of the two sequences is 13.9% and that between the remaining portions of the sequences 8.5%. The mean amino acid difference between the inferred products of the 13 peptide-coding genes is 4.4%. Sequences of the complete control regions, the complete 12S rRNA genes, the complete cytochromeb genes, and portions of the NADH4 and NADH5 genes of two other chimpanzee specimens showed that they were similar but strikingly different from the same regions of the completely sequenced molecule fromPan troglodytes verus. The two specimens were identified asPan troglodytes troglodytes, the Central African variety of the common chimpanzee.
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Anderson S, Bankier AT, Barrell BG, de Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Sequence and organisation of the human mitochondrial genome. Nature 290:457–465
Arnason U, Gullberg A, Widegren B (1991) The complete nucleotide sequence of the mitochondrial DNA of the fin whale,Balaenoptera physalus. J Mol Evol 33:556–568
Arnason U, Spilliaert R, Palsdottir A, Arnason A (1991a) Molecular identification of hybrids between the two largest whale species, the blue (Balaenoptera musculus) and the fin whale (B. physalus). Hereditas 115:183–189
Arnason U, Johnsson E (1992) The complete mitochondrial sequence of the harbour seal,Phoca vitulina. J Mol Evol 34:493–505
Arnason U, Gullberg A (1993) Comparison between the complete mtDNA sequences of the blue and the fin whale, two species that can hybridize in nature. J Mol Evol 37:312–322
Arnason U, Gullberg A, Johnsson E, Ledje C (1993) The nucleotide sequence of the mitochondrial DNA molecule of the grey seal,Halichoerus grypus, and a comparison with mitochondrial sequences of other true seals. J Mol Evol 37:323–330
Arnason U, Xu X, Gullberg A, Graur A (1996) The “Phoca standard”: an external molecular reference for calibrating recent evolutionary divergences. J Mol Evol (in press)
Foran DR, Hixson JE, Brown WM (1988) Comparison of ape and human sequences that regulate mitochondrial DNA transcription and D-loop DNA synthesis. Nucleic Acids Res 16:5841–5861
Goodman M (1963) Man's place in the phylogeny of primates as reflected in serum proteins. In: Washburn SL (ed) Classification and human evolution. Aldine Press, Chicago, pp 204–234
Goodman M (1974) Biochemical evidence on hominid phylogeny. Ann Rev Anthropol 3:203–226
Goodman M (1976) Towards a genealogical description of the primates. In: Goodman M, Tashian RE (eds) Molecular anthropology. Plenum Press, New York, pp 321–353
Hixson JE, Brown WM (1986) A comparison of the small ribosomal RNA genes from the mitochondrial DNA of great apes and humans: sequence, structure, evolution, and phylogenetic implications. Mol Biol Evol 3:1–18
Horai S, Hayasaka K, Kondo R, Tsugane K, Takahata N (1995) Recent African origin of modern humans revealed by complete sequences of hominoid mitochondrial DNAs. Proc Natl Acad Sci USA 92:532–536
Irwin DM, Kocher TD, Wilson AC (1991) Evolution of the cytochromeb gene of mammals. J Mol Evol 32:128–144
Kappelman J, Kelley J, Pilbeam D, Sheikh KA, Ward S, Anwar M, Barry JC, Brown B, Hake P, Johnson NM, Raza SM, Shah SMI (1991) The earliest occurrence ofSivapithecus from the middle Miocene Chinji Formation of Pakistan. J Hum Evol 21:61–73
Kocher TD, Wilson AC (1991) Sequence evolution in mitochondrial DNA in humans and chimpanzees: control region and a proteincoding region. In: Osawa S, Honjo T (eds) Evolution of life: fossils, molecules and culture. Springer, Tokyo, pp 391–413
Morin PA, Moore JJ, Chakraborty R, Jin L, Goodall J, Woodruff DS (1994) Kin selection, social structure, gene flow and the evolution of chimpanzees. Science 265:1193–1201
Neefs J-M, Van de Peer Y, De Rijk P, Chapelle S, De Wachter R (1993) Compilation of small ribosomal subunit RNA structures. Nucleic Acids Res 21:3025–3049
Ozawa T, Tanaka M, Sugiyama S, Ino H, Ohno K, Hattori K, Ohbayashi T, Ito T, Deguchi H, Kawamura K, Nakase Y, Hashiba K (1991) Patients with idiopathic cardiomyopathy belong to the same mitochondrial DNA gene family of Parkinson's disease and mitochondrial encephalomyopathy. Biochem Biophys Res Commun 177:518–525
Ruvolo M, Pan D, Zehr S, Goldberg TD, Disotell TR (1994) Gene trees and hominoid phylogeny. Proc Natl Acad Sci USA 91:8900–8904
Sarich VM, Wilson AC (1967) Immunological time-scale for hominid evolution. Science 158:1200–1203
Sarich VM, Cronin JE (1976) Molecular systematics of the primates. In: Goodman M, Tashian RE (eds) Molecular anthropology. Plenum Press, New York, pp 141–170
Sibley CG, Ahlquist JE (1984) The phylogeny of hominoid primates, as indicated by DNA-DNA hybridization. J Mol Evol 20:2–15
Spilliaert R, Vikingsson G, Arnason U, Palsdottir A, Sigurjonsson J, Arnason A (1991) Species hybridization between a female blue whale (Balaenoptera musculus) and a male fin whale (B. physalus): molecular and morphological documentation. J Hered 82:269–274
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Arnason, U., Xu, X. & Gullberg, A. comparison between the complete mitochondrial DNA sequences ofHomo and the common chimpanzee based on nonchimeric sequences. J Mol Evol 42, 145–152 (1996). https://doi.org/10.1007/BF02198840
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DOI: https://doi.org/10.1007/BF02198840