Skip to main content
Log in

Formation of ethanol by bacteria. A pledge for the use of extreme thermophilic anaerobic bacteria in industrial ethanol fermentation processes

  • Swiss Society of Microbiology Reports of the 39th Annual Meeting
  • Microbiology and Biochemistry of Methanogens
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Many anaerobic, facultatively anaerobic and even some strictly aerobic microorganisms form various amounts of ethanol from glucose. Only few mesophilic and extreme thermophilic bacteria ferment glucose stoichiometrically to 2 ethanol and 2 carbondioxid. Biotechnological processes at elevated temperatures seem advantageous in many respects. Hence, thermophiles and extreme thermophiles can potentially substitute for yeast in ethanol production on an industrial scale. Advantages and disadvantages of thermophilic mixed cultures and of potentially useful organisms are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. A. H. Rose and J. S. Harrison, The yeasts, vol. 3. Academic Press, London 1970.

    Google Scholar 

  2. A. H. Rose, Economic microbiology, vol. 1 and 2. Academic Press, London 1977, 1978.

    Google Scholar 

  3. A. H. Rose, The yeasts, vol. 1 and 2. Academic Press, London 1969, 1971.

    Google Scholar 

  4. J. R. Johnston and H. Oberman, in: Progress in industrial microbiology, vol. 15, p. 151. Ed. M. J. Bull. Elsevier Scientific Publ. Company, Amsterdam, Oxford, New York 1979.

    Google Scholar 

  5. J. D. Bu'Lock, 29th Symposium of the Society for General Microbiology, Cambridge 1979.

  6. A. Margaritis and Ch. R. Wilke, Biotech. Bioeng.20, 709 (1978).

    Article  CAS  Google Scholar 

  7. M. Wümpelmann and L. Kjaergaard, Biotechn. Lett.1, 365 (1979).

    Article  Google Scholar 

  8. J. Wiegel and L. G. Ljungdahl, in: Technische Mikrobiologie, p. 117. Ed. H. Dellweg. Verlag Versuchs- und Lehranstalt für Spiritusfabrikation und Fermentationstechnologie im Institut für Gärungsgewerbe und Biotechnologie, Berlin 1979.

    Google Scholar 

  9. J. Wiegel, L. G. Ljungdahl and J. R. Rawson, J. Bact.139, 800 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. E. A. Dawes, J. gen. Microbiol.32, 151 (1963).

    Article  CAS  PubMed  Google Scholar 

  11. T. E. King and V. H. Cheldelin, J. biol. Chem.208, 821 (1954).

    Article  CAS  PubMed  Google Scholar 

  12. T. Bauchop and E. A. Dawes, J. gen. Microbiol.52, 195 (1959).

    Article  Google Scholar 

  13. A. Haq and E. A. Dawes, J. gen. Microbiol.68, 295 (1971).

    Article  CAS  PubMed  Google Scholar 

  14. R. D. De Moss, J. cell. comp. Physiol.41, suppl. 1, 207 (1953).

    Google Scholar 

  15. A. H. Stouthamer, Symp. Soc. gen. Microbiol.27, 285 (1977).

    CAS  Google Scholar 

  16. A. H. Stouthamer, Int. Rev. Biochem. Microb. Biochem.21, 1 (1979).

    CAS  Google Scholar 

  17. W. E. Balch, S. Schoberth, R. S. Tanner and R. S. Wolfe, Int. J. Syst. Bact.27, 355 (1977).

    Article  CAS  Google Scholar 

  18. M. Braun, F. Mayer and G. Gottschalk, Arch. Microbiol., in press (1981).

  19. R. C. Lawrence and T. D. Thomas, Symp. Soc. gen. Microbiol.29, 187 (1979).

    CAS  Google Scholar 

  20. I. C. Gunsalus and C. F. Niven, J. biol. Chem.145, 131 (1942).

    Article  CAS  Google Scholar 

  21. J. Wiegel and L. G. Ljungdahl, Arch. Microbiol., in press (1981).

  22. A. Harden, J. chem. Soc.79, 610 (1901).

    Article  CAS  Google Scholar 

  23. H. von Hugo, Doctoral thesis, Univ. Göttingen (1973).

  24. H. von Hugo, S. Schoberth, V. K. Madan and G. Gottschalk, Arch. Mikrobiol.87, 189 (1972).

    Article  Google Scholar 

  25. J. E. L. Corry, J. appl. Bact.44 1 (1978).

    Article  CAS  Google Scholar 

  26. H. A. Barker, in: The bacteria, vol. 2, p. 151. Ed. I. C. Gunsalus and R. Y. Stanier. Academic Press, New York 1961.

    Google Scholar 

  27. G. J. Lees and G. R. Jago, J. Dairy Res.43, 75 (1976).

    Article  CAS  PubMed  Google Scholar 

  28. H. R. Hayward and T. C. stadtman, J. Bact.78, 557 (1959).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. F. D. Baker, H. R. Papiska and L. L. Campbell, J. Bact.84, 973 (1962).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. C. Bradbeer, J. biol. Chem.240, 4669 (1965).

    Article  CAS  PubMed  Google Scholar 

  31. R. Walther, Doctoral thesis, Univ. Göttingen (1977).

  32. R. Walther, H. Hippe and G. Gottschalk, Appl. environ. Microbiol.33, 955 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. M. Bogusz, M. Guminska and J. Markiewicz, Forensic Sci.1, 229 (1972).

    Article  CAS  PubMed  Google Scholar 

  34. M. Bogusz, M. Guminska and J. Markiewicz, J. forensic Med.17, 156 (1970).

    CAS  PubMed  Google Scholar 

  35. V. S. O'Leary, R. Green, G. C. Sullivan and V. H. Holsinger, Biotech. Bioeng.19, 1019 (1977).

    Article  CAS  Google Scholar 

  36. Tansey, M., ASM-news45, 417 (1979).

    Google Scholar 

  37. R. E. Buchanan and N. G. Gibbons, Bergey's manual of determinative bacteriology, 8th ed. Williams and Wilkins, Baltimore 1974.

    Google Scholar 

  38. D. Vollbrecht and M. A. El Nawawy, Eur. J. appl. Microbiol. Biotech.9, 1 (1980).

    Article  CAS  Google Scholar 

  39. W. A. Wood, in: The bacteria, vol. II, p. 50. Ed. I. C. Gunsalus and R. Y. Stanier. Academic Press, New York 1961.

    Google Scholar 

  40. H. G. Schlegel and D. Vollbrecht, J. gen. Microbiol.117, 475 (1980).

    CAS  Google Scholar 

  41. N. Kato and K. Ogata, J. Ferment Technol.51, 871 (1973).

    CAS  Google Scholar 

  42. A. Atkinson, C. G. T. Evans and R. G. Yeo, J. appl. Bact.38, 301 (1975).

    Article  CAS  Google Scholar 

  43. A. Atkinson, D. E. Ellwood, C. G. T. Evans and R. G. Yeo, Biotech. Bioeng.17, 1375 (1975).

    Article  CAS  Google Scholar 

  44. E. Kobl and J. I. Harris, Biochem. J.124, 76p (1975).

  45. L. Jung, R. Jost, E. stoll and H. Zuber, Arch. Mikrobiol.95, 125 (1974).

    Article  CAS  PubMed  Google Scholar 

  46. J. L. Stokes, J. Bact.57, 147 (1949).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. J. Swings and J. De Ley, Bact. Rev.41, 1 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. N. Okafor, J. appl. Bact.8, 81 (1975).

    Article  Google Scholar 

  49. K. J. Lee, D. E. Tribe and P. L. Rogers, Biotech. Lett.1, 421 (1979).

    Article  CAS  Google Scholar 

  50. P. L. Rogers, K. J. Lee and D. E. Tribe, Biotech. Lett.1, 165 (1979).

    Article  CAS  Google Scholar 

  51. G. Gottschalk, H. Hippe and J. R. Andreesen, in: The prokaryotes, in press (1981).

  52. L. V. Holdeman and W. E. C. Moore, in: Anaerobe Laboratory Manual, 3rd ed. Virginia Polytechnic Institute and State University, Blacksburg 1975.

    Google Scholar 

  53. L. G. Ljungdahl, in: Adv. microbiol. Physiol.19, 149. Ed. A. H. Rose and J. G. Morris. Academic Press, London 1979.

    Google Scholar 

  54. R. E. Amelunxen and A. L. Murdock, CCR Crit. Rev. Microbiol.6, 343 (1978).

    Article  CAS  Google Scholar 

  55. M. Friedman, Biochemistry of thermophily. Academic Press, New York 1979.

    Google Scholar 

  56. J. G. Zeikus, Env. Microbiol. Tech.1, 243 (1979).

    Article  CAS  Google Scholar 

  57. D. G. Cooney and R. Emerson, Thermophilic fungi. An account of their biology, activities and Classification. W. H. Freeman, San Francisco, London 1964.

    Google Scholar 

  58. J. G. Zeikus and R. S. Wolfe, J. Bact.109, 707 (1972).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  59. H. Klaushofer and E. Parkinen, Z. Zuckerind. Böhm.15, 445 (1965).

    CAS  Google Scholar 

  60. J. G. Zeikus, P. W. Hegge and M. A. Anderson, Arch. Microbiol.122, 41 (1979).

    Article  CAS  Google Scholar 

  61. S. H. Zinder and R. A. Mah, Appl. environ. Microbiol.38, 996 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  62. S. Schoberth, Abstr. J 18, Ann. Meet. Am. Soc. Microbiol. Los Angeles 1979.

  63. J. R. Postgate and L. L. Campbell, Bact. Rev.30, 732 (1966).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  64. L. G. Ljungdahl, S.-S. Yang, M.-T. Liu, J. Wiegel and F. Mayer, in: Biochemistry of thermophily, p. 385. Ed. Friedmann. Academic Press Inc. New York 1978.

    Chapter  Google Scholar 

  65. J. G. Zeikus, A. Ben-Bassat and P. W. Hegge, J. Bact.143, 432 (1980).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  66. F. Hollaus and U. Sleytr, Arch. Mikrobiol.86, 129 (1972).

    Article  CAS  PubMed  Google Scholar 

  67. S. C. Prescott and C. G. Dunn, in: Industrial microbiology, p. 250. McGraw-Hill, New York 1959.

    Google Scholar 

  68. J. Wiegel and L. G. Ljungdahl, Abstr. K73, A. Meet. Am. Soc. Microbiol. Los Angeles 1979.

  69. W. A. Mercer and R. H. Vaugh, J. Bact.62, 27 (1951).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  70. D. Matteuzzi, F. Hollaus and B. Biavati, Int. J. Syst. Bact.28, 528 (1978).

    Article  Google Scholar 

  71. K. T. Yasunobu and M. Tanaka, in: Iron sulfur proteins, vol. 2, p. 27. Ed. W. Lovenberg. Academic Press, New York 1973.

    Google Scholar 

  72. S.-S. Yang, L. G. Ljungdahl and J. Le Gall, J. Bact.130, 1084 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  73. L. S. McClung, J. Bact.29, 189 (1935).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  74. N. O. Sjolander, J. Bact.34, 419 (1937).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  75. C. K. Lee and Z. J. Ordal, J. Bact.94, 530 (1967).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  76. E. J. Hsu and Z. J. Ordal, J. Bact.102, 369 (1970).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  77. M. Ingraham, in: The bacterial spores, p. 549. Ed. G. W. Gould and A. Hurst. Academic Press, London, New York 1969.

    Google Scholar 

  78. D. I. C. Wang, I. Biocic, H.-Y. Fang and S.-D. Wang 3rd Ann. Biomass Energy Systems Conference Proceedings, p. 61 (1979).

  79. R. H. McBee, Bact. Rev.14, 51 (1950).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  80. L. Enebo, Ph. D. thesis, Royal Institute of Technology Stockholm, Sweden (1954).

  81. N. J. Patni and J. K. Alexander, J. Bact.105, 220 (1971).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  82. N. J. Patni and J. K. Alexander, J. Bact.105, 226 (1971).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  83. B. H. Lee and T. H. Blackburn, Appl. Microbiol.30, 346 (1975).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  84. T. K. Ng, P. J. Weimar and J. G. Zeikus, Arch. Microbiol.114, 1 (1977).

    Article  CAS  PubMed  Google Scholar 

  85. L. Y. Quinn, Ph.D. thesis, Puerdue Univ. Lafayette, Ind. USA (1949).

  86. J. A. Viljoens, E. B. Fred and W. H. Peterson, Agric. Sci.1, 17 (1926).

    Google Scholar 

  87. R. F. Gomez, in: Proceedings Colloque Cellulyse Microbienne, Centre National de la Recherche Scientifique, Marseille 1980.

  88. P. J. Weimar and J. G. Zeikus, Appl. environ. Microbiol.33, 289 (1977).

    Article  Google Scholar 

  89. J. G. Zeikus, P. W. Hegge and M. A. Anderson, Arch. Microbiol.122, 41 (1979).

    Article  CAS  Google Scholar 

  90. V. M. Varel, H. R. Isaacson and M. P. Bryant, Appl. environ. Microbiol.33, 298 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  91. E. Canale-Parola, Bact. Rev.41, 181 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  92. D. D. Sutton and M. P. Starr, J. Bact.78, 427 (1959).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  93. S. L. Rosenberg, Enzym. Microbiol. Technol.2, 185 (1980).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Acknowledgment. This work was partly supported by Energy and Research Development Administration contract number EY-76-509-0888-M003 and by the Deutsche Forschungsgemeinschaft.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wiegel, J. Formation of ethanol by bacteria. A pledge for the use of extreme thermophilic anaerobic bacteria in industrial ethanol fermentation processes. Experientia 36, 1434–1446 (1980). https://doi.org/10.1007/BF01960144

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01960144

Keywords

Navigation