Abstract
The autophosphorylation-dependent protein kinase has been identified as a potent vimentin kinase that incorporates 2 mol of phosphates per mol of protein and generates five major phosphorylation sites in vimentin. Tryptic phosphopeptide mapping by high-performance liquid chromatography followed by sequential manual Edman degradation and direct peptide sequence analysis revealed that Ser-25, Ser-38, Ser-65, and Ser-71 in the amino-terminal domain and Ser-411 in the carboxyl-terminal domain are the phosphorylation sites in vimentin phosphorylated by this kinase, indicating that autophosphorylation-dependent protein kinase is a potent and unique vimentin kinase. Functional study further revealed that phosphorylation of vimentin by autophosphorylation-dependent protein kinase can completely inhibit polymerization and assembly of the cytoskeletal intermediate filament as demonstrated by electron microscopic analysis. Taken together, the results provide initial evidence that the autophosphorylation-dependent protein kinase may function as a vimentin kinase involved in the structure-function regulation of the cytoskeletal system. The results also support the notion that this cyclic nucleotide- and calcium-independent protein kinase may function as a multisubstrate/multifunctional protein kinase involved in the regulation of diverse cell functions.
Similar content being viewed by others
References
Andersson, L., and Porath, J. (1986).Anal. Biochem. 154, 250–254.
Ando, S., Tanabe, K., Gonda, Y., Sato, C., and Inagaki, M. (1989).Biochemistry 28, 2974–2979.
Ando, S., Tokui, T., Yamauchi, T., Sugiura, H., Tanabe, K., and Inagaki, M. (1991).Biochem. Biophys. Res. Commun. 150, 1006–1011.
Boyle, W. J., van der Geer, P., and Hunter, T. (1991)Meth. Enzymol. 201, 110–149.
Chou, Y.-H., Bischoff, J. R., Beach, D., and Goldman, R. D. (1990).Cell 62, 1063–1071.
Chou, Y.-H., Ngai, K.-L., and Goldman, R. D. (1991)J. Biol. Chem. 266, 7327–7328.
Cohen, P., Parker, P. J., and Woodgett, J. R. (1985). InMolecular Basis of Insulin Action (Czech, M. P., ed.) Plenum Press, New York, pp. 213–233.
De la Houssaye, B. A., Echols, T. K., and Masaracchia, R. A. (1983).J. Biol. Chem. 258, 4272–4278.
Eylar, E. H., Salk, J., Beveridge, G. C., and Brown, L. V. (1969).Arch. Biochem. Biophys. 132, 34–48.
Flockhart, D. A., and Corbin, J. D. (1982).CRC Crit. Rev. Biochem. 12, 134–186.
Geisler, N., Hatzfeld, M., and Weber, K. (1989).Eur. J. Biochem. 183, 441–447.
Guo, H., and Damuni, Z. (1993).Proc. Natl. Acad. Sci USA 90, 2500–2504.
Guo, H., Reddy, S. A. G., and Damuni, Z. (1993)J. Biol. Chem. 268, 11193–11198.
Hathaway, G. M., and Traugh, J. A. (1982).Curr. Top. Cell. Regul. 21, 101–127.
Hennekes, H., Kuehn, S., and Traub, P. (1990)Mol. Gen. Genet. 221, 33–36.
Ingaki, M., Nishi, Y., Nishizawa, K., Matsuyama, M., and Sato, C. (1987).Nature 328, 649–652.
Ingaki, M., Gonda, Y., Matsuyama, M., Nishizawa, K., Nishi, Y., and Sato, C. (1988).J. Biol. Chem. 263, 5970–5978.
Krebs, E. G., and Beavo, J. A. (1979).Annu. Rev. Biochem. 48, 923–959.
Laemmli, U. K. (1970).Nature 227, 680–685.
Laursen, R. A. (1966).J. Am. Chem. Soc. 88, 5344–5346.
Laursen, R. A., and Machleidt, W. (1980). InMethods of Biochemical Analysis, Vol. 26 (Glick, D. ed.), Wiley, New York, pp. 201–284.
Merdes, A., Brunkener, M., Horstmann, H., and Georgatos, S. D. (1991).J. Cell Biol. 115, 397–410.
Nairn, A. C., Hemmings, H. C., and Greengard, P. (1985).Annu. Rev. Biochem. 54, 931–976.
Ramakrishna, S., and Benjamin, W. B. (1985).J. Biol. Chem. 260, 12280–12286.
Reimann, E. M., Walsh, D. A., and Krebs, E. G. (1971).J. Biol. Chem. 246, 1986–1995.
Roach, P. J. (1984).Meth. Enzymol. 107, 81–101.
Wood, L., Theriault, N., and Vogel, G. (1989).Gene 76, 171–175.
Wyatt, T. A., Lincoln, T. M., and Pryzwansky, K. B. (1991).J. Biol. Chem. 266, 21274–21280.
Yang, D.-D., Fong, Y.-L., Yu, J.-S., and Liu, J.-S., (1987a).J. Biol. Chem. 262, 7034–7040.
Yang, S.-D., Cheng, S.-Y., and Soderling, T. R., (1987b).J. Biol. Chem. 262, 9421–9427.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huang, TJ., Lee, TT., Lee, WC. et al. Autophosphorylation-dependent protein kinase phosphorylates Ser25, Ser38, Ser65, Ser71, and Ser411 in vimentin and thereby inhibits cytoskeletal intermediate filament assembly. J Protein Chem 13, 517–525 (1994). https://doi.org/10.1007/BF01901533
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01901533