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Effects of indole-3-acetic acid on croton oil- and arachidonic acid-induced mouse ear edema

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Abstract

The indole-3-acetic acid (IAA) is a plant growth hormone (auxin) being considered as a tryptophan metabolite in animals. The main purpose of this work was to verify IAA's topical anti-inflammatory action using croton oil- or arachidonic acid-induced mouse ear edema, in comparison to known anti-inflammatory agents. IAA antioxidant activity was also verified by measuring the inhibition of brain homogenate lipid peroxidation with the thiobarbituric acid reactive substances (TBARS) test. IAA inhibited the action of both croton oil- and arachidonic acid-induced edema in a dose-dependent manner (4.0 µmoles IAA inhibited 75.8% in croton oil and 82.5% in arachidonic acid induced ear edema). Both IAA (5.3 mM) and indomethacin (8.0 mM) inhibited TBARS formation. Data suggest that IAA exhibits antiinflammatory effect possibly by its antioxidant activity.

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Authors and Affiliations

  1. Department of Physiology, Biosciences Institute, University of São Paulo, Rua do Matão, Travessa 14, No 101, 05508-900, São Paulo, Brazil

    L. H. Jones & J. C. Freitas

  2. Department of Clinical and Toxicological Analyses, Pharmaceutical Sciences Faculty, University of São Paulo, Brazil

    D. S. P. Abdalla

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  1. L. H. Jones
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  3. J. C. Freitas
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Jones, L.H., Abdalla, D.S.P. & Freitas, J.C. Effects of indole-3-acetic acid on croton oil- and arachidonic acid-induced mouse ear edema. Inflamm Res 44, 372–375 (1995). https://doi.org/10.1007/BF01797863

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  • DOI: https://doi.org/10.1007/BF01797863

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