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Inhibitory factors involved in acetone-butanol fermentation byClostridium saccharoperbutylacetonicum

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Abstract

Studies carried out onClostridium saccharoperbutylacetonicum (ATCC 27022) reveal that intracellular and extracellular inhibitors, including metabolic end-products, caused the inhibition of cell growth and solvent production. Butanol at the level of 13.0 g/liter was completely inhibitory to the growth of cells, whereas butyric acid totally inhibited the cell growth at a concentration of 8.7 g/liter. Investigations carried out on the effect of addition of culture filtrate concentrate and cell-free extract concentrate indicate that nonvolatile inhibitors produced by cells were also inhibitory for bioconversion. The butanol production was found to be reduced by 15%–20% on addition of cell-free extract concentrate. Inhibition by concentrates was enhanced in the presence of butanol. Addition of heat-sterilized concentrates resulted in a reduction of inhibition.

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Author notes

  1. Bhupinder K. Soni

    Present address: Department de Genie Biochemique et Alimentaire, INSA, 31077 Avenue de Rangueil, Toulouse, Cedex, France

Authors and Affiliations

  1. Biochemical Engineering Research Center, Indian Institute of Technology, Delhi, Haus Khas, 110016, New Delhi, India

    Bhupinder K. Soni,  Kumudeswar Das & Tarun K. Ghose

Authors
  1. Bhupinder K. Soni
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  2. Kumudeswar Das
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  3. Tarun K. Ghose
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Soni, B.K., Das, K. & Ghose, T.K. Inhibitory factors involved in acetone-butanol fermentation byClostridium saccharoperbutylacetonicum . Current Microbiology 16, 61–67 (1987). https://doi.org/10.1007/BF01588173

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