Summary
Aedes albopictus clone C6/36 cells were adapted to suspension culture in spinner flasks, and largescale cell cultures were achieved. Suspension cultured cells, attached on microcarriers, were inoculated with Japanese encephalitis virus. Virus-infected fluid with 107 to 108 plaque-forming units/ml was harvested daily from Days 2 to 8 postinfection. This method makes it possible to prepare large amounts of purified virions with less culture space and labor.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Deubel, V.; Kinney, R. M.; Trent, D. W. Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 2 virus, Jamaica genotype: comparative analysis of the full-length genome. Virology 165:234–244; 1988.
Igarashi, A. Isolation of Singh'sAedes albopictus cell clone sensitive to dengue and chikungunya viruses. J. Gen. Virol. 40:531–544; 1978.
Morita, K.; Igarashi, A. Oligonucleotide fingerprint analysis of strains of Getah virus isolated in Japan and Malaysia. J. Gen. Virol. 65:1899–1908; 1984.
Sumiyoshi, H.; Mori, C.; Fuke, I., et al. Complete nucleotide sequence of the Japanese encephalitis virus genome RNA. Virology 161:497–510; 1987.
Trent, D. W.; Grant, J. A.; Rosen, L., et al. Genetic variation among dengue 2 viruses of different geographic origin. Virology 128:271–284; 1983.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Morita, K., Igarashi, A. Suspension culture ofAedes albopictus cells for flavivirus mass production. Journal of Tissue Culture Methods 12, 35–37 (1989). https://doi.org/10.1007/BF01578006
Issue Date:
DOI: https://doi.org/10.1007/BF01578006