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Replication of echo virus type 25 JV-4 reference strain and wild type strains in MRC5 cells compared with that of poliovirus type 1

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Summary

In echo virus type 25/JV-4 the shut off of host cell protein synthesis took significantly longer and the kinetics of the synthesis of viral proteins and viral RNA occurred much later than in the poliovirus. However, these characteristics impaired neither polyprotein processing nor virus production in the JV-4 strain. In contrast the two wild strains M.1262 and Th.222 had a lower virus yield than strain JV-4. The presence of a high Mr protein in the pattern of viral proteins of wild strains suggested that a defect in the polyprotein processing was responsible for the decreased virus yield. The infectious cycle of strain Th.222 differed from that of strains JV-4 and M.1262 in the rapid inhibition of host cell translation and the extent of viral protein synthesis. The sensitivity to actinomycin D was also investigated. Strain M.1262 was found to be insensitive. The virus yield of strains JV-4 and Th.222 was three- and fourfold lower respectively in the presence of actinomycin D. This sensitivity to the antibiotic was observed during viral RNA synthesis in strain JV-4 and during viral protein synthesis in strain Th.222. These results suggest that cellular factors are involved in the replication of echo virus type 25 strains in MRC5 cells.

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References

  1. Agol VI (1991) The 5′-untranslated region of picornaviral genomes. Adv Virus Res 40: 103–180

    Google Scholar 

  2. Bailly JL, Chambon M, Peigue-Lafeuille H, Laveran H, De Champs C, Beytout D (1991) Activity of glutaraldehyde at low concentrations (<2%) against poliovirus and its relevance to gastrointestinal endoscope disinfection procedures. Appl Environ Microbiol 57: 1156–1160

    Google Scholar 

  3. Barbi Guidotti M (1983) An outbreak of skin rash by echovirus 25 in an infant home. J Infect 6: 67–70

    Google Scholar 

  4. Bell EJ, Grist NR, Russel SJM (1965) Echovirus 25 infections in Scotland, 1961–1964. Lancet 2: 464–466

    Google Scholar 

  5. Carrasco L, Castrillo JL (1987) The regulation of translation in picornavirus-infected cells. In: Pevear DC, Calenoff M, Rozhon E, Lipton HL (eds) Mechanisms of viral toxicity in animal cells. CRC Press, Boca Raton, pp 115–146

    Google Scholar 

  6. Chambon M, Bailly JL, Peigue-Lafeuille H (1992) Activity of glutaraldehyde at low concentrations against capsid proteins of poliovirus type 1 and echovirus type 25. Appl Environ Microbiol 58: 3517–3521

    Google Scholar 

  7. Chambon M, Bailly JL, Peigue-Lafeuille H (1994) Comparative sensitivity of the echo virus type 25 JV-4 prototype strain and two recent isolates to glutaraldehyde at low concentrations. Appl Environ Microbiol 60: 387–392

    Google Scholar 

  8. Cooper PD (1966) The inhibition of poliovirus growth by actinomycin D and the prevention of the inhibition by pretreatment of the cells with serum or insulin. Virology 28: 663–678

    Google Scholar 

  9. Chang SL, Berg G, Busch KA, Stevenson RE, Clarke NA, Kabler PW (1958) Application of the “most probable number” method for estimating concentrations of animal viruses by the tissue culture technique. Virology 6: 27–42

    Google Scholar 

  10. Del Angel RM, Papavassiliou AG, Fernandez-Thomas C, Silverstein SJ, Racaniello VR (1989) Cell proteins bind to multiple sites within the 5′ untranslated region of poliovirus RNA. Natl Acad Sci USA 86: 8299–8303

    Google Scholar 

  11. Dildine SL, Semler BL (1992) Conservation of RNA-Protein interactions among picornaviruses. J Virol 66: 4364–4376

    Google Scholar 

  12. Gebhard JR, Ehrenfeld E (1992) Specific interactions of HeLa cell proteins with proposed translation domains of the poliovirus 5′ noncoding region. J Virol 66: 3101–3109

    Google Scholar 

  13. Grado C, Fischer S, Contreras G (1965) The inhibition by actinomycin D of poliovirus multiplication in HEp 2 cells. Virology 27: 623–625

    Google Scholar 

  14. Hellen CUT, Fäcke M, Kräusslich HG Lee CK, Wimmer E (1991) Characterization of poliovirus 2A proteinase by mutational analysis: residues required for autocatalytic activity are essential for induction of cleavage of eukaryotic initiation factor 4F polypeptide p220. J Virol 65: 4226–4231

    Google Scholar 

  15. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685

    Google Scholar 

  16. McKinney RE Jr, Katz SL, Wilfert CM (1987) Chronic enteroviral meningoencephalitis in agammaglobulinemic patients. Rev Infect Dis 9: 334–356

    Google Scholar 

  17. Melnick JL, Dalldorf G, Enders JF, Gelfand HM, Mc D Hammon W, Huebner RJ, Rosen L, Sabin AB, Syverton JT, Wenner HA (1962) Classification of human enteroviruses. Virology 16: 501–504

    Google Scholar 

  18. Melnick JL (1990) Enteroviruses: Polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In: Fields BN, Knipe DM (eds) Virology, 2nd edn. Raven Press, New York, pp 549–605

    Google Scholar 

  19. Moritsugu Y, Sawada K, Hinohara M, Tsushiya K, Tagaya I, Hirayama M, Futaki T (1968) An outbreak of type 25 echovirus infection with exanthem in an infant home near Tokyo. Am J Epidemiol 87: 599–608

    Google Scholar 

  20. Nottay BK, Kew OM, Hatch MH, Heyward JT, Obijeski JF (1981) Molecular variation of type 1 vaccine-related and wild polioviruses during replication in humans. Virology 108: 405–423

    Google Scholar 

  21. Pallansch MA, Kew OM, Semler BL, Omilianowski DR, Anderson CW, Wimmer E, Rueckert RR (1984) Protein processing map of poliovirus. J Virol 49: 873–880

    Google Scholar 

  22. Peigue-Lafeuille H, Fuchs F, Gharabaghi F, Chambon M, Aymard M (1990) Impact on routine diagnosis of echovirus infections of intratypic differentiation and antigenic variation in echovirus type 25 studied by using monoclonal antibodies. J Clin Microbiol 28: 2291–2296

    Google Scholar 

  23. Peigue-Lafeuille H, Bailly JL, Fuchs F, Chambon M, Aymard M (1991) Heterogeneity of capsid proteins of echovirus type 25 wild-type strain and prototype strain, studied by using sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting. J Clin Microbiol 29: 1780–1784

    Google Scholar 

  24. Porter AG (1993) Picornavirus nonstructural proteins: emerging roles in virus replication and inhibition of host cell functions. J Virol 67: 6917–6921

    Google Scholar 

  25. Racaniello VR, Meriam C (1986) Poliovirus temperature-sensitive mutant containing a single nucleotide deletion in the 5′-noncoding region of the viral RNA. Virology 155: 498–507

    Google Scholar 

  26. Rosen L, Kern J, Bell JA (1964) Observations on an outbreak of infection with a newly recognized enterovirus (JV-4). Am J Hyg 79: 1–6

    Google Scholar 

  27. Rueckert RR, Wimmer E (1984) Systematic nomenclature of picornavirus proteins. J Virol 50: 957–959

    Google Scholar 

  28. Rueckert RR (1990) Picornaviridae and their replication. In: Fields BN, Knipe DM (eds) Virology. Raven Press, New York, pp 507–548

    Google Scholar 

  29. Simoes EAF, Sarnow P (1991) An RNA hairpin at the extreme 5′ end of the poliovirus RNA genome modulates viral translation in human cells. J Virol 65: 913–921

    Google Scholar 

  30. Trono D, Andino R, Baltimore D (1988) An RNA sequence of hundreds of nucleotides at the 5′ end of poliovirus RNA is involved in allowing viral protein synthesis. J Virol 62: 2291–2299

    Google Scholar 

  31. Van Der Sar A (1979) Acute infectious lymphocytosis with echovirus type 25. West Indian Med J 28: 185–188

    Google Scholar 

  32. Wiegers KJ, Dernick R (1981) Poliovirus-specific polypeptides in infected HeLa cells analysed by isoelectric focusing and 2D-analysis. J Gen Virol 52: 61–69

    Google Scholar 

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Authors and Affiliations

  1. Service de Bactériologie-Virologie, Faculté de Médecine, Clermont-Ferrand, France

    J. -L. Bailly, M. Chambon, H. Peigue-Lafeuille & F. Charbonné

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  1. J. -L. Bailly
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  2. M. Chambon
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  3. H. Peigue-Lafeuille
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  4. F. Charbonné
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Bailly, J.L., Chambon, M., Peigue-Lafeuille, H. et al. Replication of echo virus type 25 JV-4 reference strain and wild type strains in MRC5 cells compared with that of poliovirus type 1. Archives of Virology 137, 327–340 (1994). https://doi.org/10.1007/BF01309479

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  • DOI: https://doi.org/10.1007/BF01309479

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