Skip to main content
SpringerLink
Log in

Role of endogenous secretin and cholecystokinin in intraduodenal oleic acid-induced inhibition of gastric acid secretion in rats

  • Original Article
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

We investigated a possible role of endogenous secretin and cholecystokinin (CCK) in inhibition of gastric acid secretion induced by intraduodenal administration of oleic acid in rats. Intraduodenal administration of oleic acid emulsion in a dose of 1 mmol/hr resulted in significant inhibition of gastric acid secretion stimulated by intravenous infusion of pentagastrin (0.3 μg/kg/hr), and this was accompanied by an increase in the plasma concentration of both secretin and CCK, from 1.2±0.08 pM and 20.6±1.2 pM to 4.3±0.18 pM and 31.6±0.9 pM, respectively (P<0.001). Intravenous infusion of secretin (0.05 CU/kg/hr) inhibited pentagastrin-stimulated gastric acid secretion, but CCK-8 (0.03 μ/kg/hr) failed, although intravenous infusion of secretin, and CCK in those doses produced plasma levels comparable to the levels achieved in response to oleic acid administration. Furthermore, the oleic acid-induced suppression of gastic acid secretion was blocked significantly by intravenous injection of rabbit anti-secretin serum (0.1 ml), but not by intravenous infusion of a CCK-receptor antagonist, CR 1409 (5 mg/kg/hr). Thus, the results of this study indicate that endogenous secretin rather than CCK is involved in the hormonal mechanism regulating the inhibition of gastric acid secretion by intestinal fat in rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Grossman MI: Regulation of gastric acid secretion.In Physiology of the Gastrointestinal Tract, LR Johnson (ed). New York, Raven Press, 1981, pp 659–671

    Google Scholar 

  2. Kosaka T, Lim RKS: Demonstration of the humoral agent in fat inhibition of gastric secretion. Proc Soc Exp Biol Med 27:890–891, 1930

    Google Scholar 

  3. Kosaka T, Lim RKS: On the mechanism of the inhibition of gastric secretion by fat: The role of bile and cystokinin. Chin J Physiol 4:213–220, 1930

    Google Scholar 

  4. Watanabe S, Chey WY, Lee KY, Chang T-M: Secretin is released by digestive products of fat in dogs. Gastroenterology 90:1008–1017, 1986

    Google Scholar 

  5. Shaffalitzky de Muckadell OB, Olsen O, Canto P, Magid E: Concentrations of secretin and CCK in plasma and pancreaticobiliary secretion in response to intraduodenal acid and fat. Pancreas 1:536–543, 1986

    Google Scholar 

  6. Shiratori K, Jo YH, Lee KY, Chang TM, Chey WY: Effect of pancreatic juice and trypsin on oleic acid-stimulated pancreatic secretion and plasma secretin in dogs. Gastroenterology 96:1330–1336, 1989

    Google Scholar 

  7. Fried GM, Ogden WD, Swierczek J, Greeley GH, Rayford PL, Thompson JC: Release of cholecystokinin in conscious dogs: Correlation with simultaneous measurements of gallbladder pressure and pancreatic protein secretion. Gastroenterology 85:1113–1119, 1983

    Google Scholar 

  8. Chey WY, Sivasomboon B, Hendricks J: Actions and interactions of gut hormones and histamine on gastric secretion of acid in the rat. Am J Physiol 224:852–856, 1973

    Google Scholar 

  9. Johnson LR, Grossman MI: Secretin: The enterogastrone released by acid in the duodenum. Am J Physiol 215:885–888, 1968

    Google Scholar 

  10. Chey, WY, Kim MS, Lee KY, Chang TM: Secretin is an enterogastrone in the dog. Am J Physiol 240:G239-G244, 1981

    Google Scholar 

  11. Kim YC, Lee KY, Chey WY: Role of secretin on postprandial gastrin release in the dog: A further study. Surgery 90:504–508, 1981

    Google Scholar 

  12. Chey WY, Hitanant S, Hendricks J, Lorber SH: Effect of secretin and cholecystokinin on gastric emptying and gastric secretion in man. Gastroenterology 58:820–827, 1970

    Google Scholar 

  13. Shiratori K, Watanabe S, Takeuchi T: Physiological significance of secretin on gastric secretion. Jpn J Gastroenterol 81:1737–1741, 1984

    Google Scholar 

  14. You CH, Chey WY: Secretin is an enterogastrone in humans. Dig Dis Sci 32:466–471, 1987

    Google Scholar 

  15. Shiratori K, Watanabe S, Takeuchi T, Shimizu K: Plaunotol inhibits postprandial gastrin release by its unique secretin-releasing action in humans. Dig Dis Sci 35:1140–1145, 1990

    Google Scholar 

  16. Seal AM, Meloche RM, Liu YQE, Buchan AMJ, Brown JC: Effects of monoclonal antibodies to somatostatin on somatostatin-induced and intestinal fat-induced inhibition of gastric acid in the rat. Gastroenterology 92:1187–1192, 1987

    Google Scholar 

  17. Green GM, Taguchi S, Friestman J, Chey WY, Liddle RA: Plasma secretin, CCK, and pancreatic secretion in response to dietary fat in the rat. Am J Physiol 256:G1016-G1021, 1989

    Google Scholar 

  18. Shiratori K, Watanabe S Takeuchi T, Shimizu K, Moriyoshi Y: Role of secretin and cholecystokinin in oleic acidstimulated pancreatic secretion in rats. Gastroenterol Jpn 25:104–111, 1990

    Google Scholar 

  19. Li P, Lee KY, Ren XS, Chang T-M, Chey WY: Effect of pancreatic proteases on plasma cholecystokinin, secretin, and pancreatic exocrine secretion in response to sodium oleate. Gastroenterology 98:1642–1648, 1990

    Google Scholar 

  20. Ghosh MN, Schild HO: Continuous recording of acid gastric secretion in the rat. Brt J Pharmacol 13:54–61, 1958

    Google Scholar 

  21. Lai KS: Studies on gastrin,. Gut 5:327–333, 1964

    Google Scholar 

  22. Chang T-M, Chey WY: Radioimmunoassay of secretin. A critical review and current status. Dig Dis Sci 25:529–551, 1980

    Google Scholar 

  23. Makovec F, Chiste R, Bani M, Pacini MA, Setnikar I, Rovati LA: New glutaramic acid derivatives with potent competitive and specific cholecystokinin-antagonistic activity. Drug Res 35:1048–1051, 1985

    Google Scholar 

  24. Watanabe S, Shiratori K, Takeuchi T: Radioimmunoassay of secretin using ethanol extraction method of plasma: Significant increase of plasma secretin concentrations after a meal in man. Jpn J Gastroenterol 78:1920–1928, 1981

    Google Scholar 

  25. Shiratori K, Watanabe S, Takeuchi T: Radioimmunoassay of secretin with special reference to examination of its sensitivity. Jpn J Gastroenterol 80:1475–1479, 1983

    Google Scholar 

  26. Shiratori K, Watanabe S, Takeuchi T: Radiommunoassay of cholecystokinin using XAD-2 resin extraction method of plasma: Plasma cholecystokinin responses after a meal and intraduodenal ingestion of MgSO4 in humans. Jpn J Gastroenterol 82:277–287, 1985

    Google Scholar 

  27. Chang T-M, Chey WY: Radioimmunoassay of cholecystokinin. Dig Dis Sci 28:456–468, 1983

    Google Scholar 

  28. Chey WY, Kim MS, Lee KY., Chang T-M: Effect of rabbit antisecretin serum on pastprandial pancreatic secretion in dogs. Gastroenterology 77:1268–1275, 1979

    Google Scholar 

  29. Shiratori K, Shimizu K, Watanabe S, Takeuchi T, Moriyoshi Y: Effect of CCK antagonists CR 1409 and CR 1505 on rat pancreatic exocrine secretionin vivo. Pancreas 4:744–750, 1989

    Google Scholar 

  30. Colturi TJ, Unger RH, Feldman M: Role of circulating somatostatin in regulation of gastric acid secretion, gastrin release and islet cell function: Studies in healthy subjects and duodenal ulcer patients. J Clin Invest 74:417–423, 1984

    Google Scholar 

  31. Guo Y-S, Singh P, Gomez G, Greeley GH Jr, Thompson JC: Effect of peptide YY on cephalic, gastric, and intestinal phase of gastric acid secretion and on the release of gastrointestinal hormones. Gastroenterology 92:1202–1208, 1987

    Google Scholar 

  32. Mogard MH, Maxwell V, Wong H, Reedy TJ, Sytnik B, Walsh JH: Somatostatin may not be a hormonal messenger of fat-induced inhibition of gastric functions. Gastroenterology 94:405–408, 1988

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine, Tokyo Women's Medical College, Tokyo, Japan

    Keiko Shiratori MD, Shin-Ichiro Watanabe MD & Tadashi Takeuchi MD, PhD

  2. Department of Gastroenterology, Tokyo Women's Medical College, 8-1 Kawada-cho, Shinjuku-ku, 162, Tokyo, Japan

    Keiko Shiratori MD, Shin-Ichiro Watanabe MD & Tadashi Takeuchi MD, PhD

Authors
  1. Keiko Shiratori MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shin-Ichiro Watanabe MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tadashi Takeuchi MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported in part by a grant from the Japanese Ministry of Education. Part of this work was presented at the Annual Meeting of the American Gastroenterological Association, May 12–18, 1990, San Antonio, and appeared in abstract form inGastroenterology 98:A124, 1990

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shiratori, K., Watanabe, SI. & Takeuchi, T. Role of endogenous secretin and cholecystokinin in intraduodenal oleic acid-induced inhibition of gastric acid secretion in rats. Digest Dis Sci 37, 397–402 (1992). https://doi.org/10.1007/BF01307734

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01307734

Key Words

Search

Navigation