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The primary structure of papaya mosaic virus coat protein: A revision

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Abstract

The presence of an acetyl blocking group at theN-terminus of the coat protein of papaya mosaic virus has been identified by FAB mass spectrometry. Furthermore, we have found that theN-terminal sequence of the protein is four amino-acid residues (AC-Ser-Lys-Ser-Ser-) longer than that previously reported, while Glu instead of Gln is theC-terminal residue. The present paper shows that PMV-protein is made up of 215 amino acid residues, with a molecular mass of 22,960 Da.

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References

  • Acharya, A. S., Di Donato, A., Manjula, B. N., Fischetti, V. A., and Manning, J. M. (1983).Int. J. Peptide Protein Res. 22, 78–82.

    Article  CAS  Google Scholar 

  • D'Alessio, G., Parente, A., Farina, B., La Montagna, R., De Prisco, R., Demma, G. B., and Leone, E. (1972).Biochem. Biophys. Res. Commun. 47, 293–299.

    Article  CAS  PubMed  Google Scholar 

  • Erickson, J. W., Bancroft, J. B., and Horne, R. W. (1976).Virology 72, 514–517.

    Article  CAS  PubMed  Google Scholar 

  • Erickson, J. W., and Bancroft, J. B. (1978).Virology 90, 36–46.

    Article  CAS  PubMed  Google Scholar 

  • Gibbs, A., and Harrison, B. (1976). InPlant Virology: The Principles, Arnold, Butler & Tanner, London.

    Google Scholar 

  • Harrison, B. D., Finch, J. T., Gibbs, A. J., Hollings, M., Shepherd, R. J., Valento, V., and Wetter, C. (1971).Virology 45, 356–363.

    Article  CAS  PubMed  Google Scholar 

  • Jones, B. N. (1986). InMethods of Proteins Microcharacterization (Shively, J. E., ed.), Humana Press, Clifton, New Jersey, pp. 337–361.

    Chapter  Google Scholar 

  • Lago, C. T., Sannia, G., Marino, G., Squires, C. H., Calvo, J. M., and De Felice, M. (1985).Biochim. Biophys. Acta 824, 74–79.

    Article  CAS  PubMed  Google Scholar 

  • Malorni, A., Milone, A., and Marino, G. (1986).Biomed Environ. Mass Spectrom. 13, 477–482.

    Article  CAS  PubMed  Google Scholar 

  • Parente, A., Short, M. N., Self, R., and Parsley, K. R. (1982).Biomed. Mass Spectrom. 9, 141–145.

    Article  CAS  PubMed  Google Scholar 

  • Penke, B., Ferenczi, R., and Kovaks, K. (1974).Anal. Biochem. 60, 45–50.

    Article  CAS  PubMed  Google Scholar 

  • Petrilli, P. (1988).CABIOS 4, 265–270.

    CAS  PubMed  Google Scholar 

  • Pucci, P., Sannia, G., and Marino, G. (1981).J. Chromatogr. 270, 371–377.

    Article  Google Scholar 

  • Rees, M. W., Parente, A., and Short, M. N. (1978).John Innes Annual Report, 1978, pp. 93–97.

  • Sawyer, L., Tollin, P., and Wilson, H. R. (1987).J. Gen. Virol. 68, 1229–1232.

    Article  CAS  Google Scholar 

  • Short, M. N., Turner, D. S., March, J. F., Pappin, D. J. C., Parente, P., and Davies, J. W. (1986).Virology 152, 280–283.

    Article  CAS  PubMed  Google Scholar 

  • Stein, W. H., and Moore, S. (1963).Methods Enzymol. 6, 819–831.

    Article  Google Scholar 

  • Tarr, G. E. (1982). InMethods in Protein Sequence Analysis (Elzinga, M., ed.), Humana Press, Clifton, New Jersey, pp. 223–232.

    Chapter  Google Scholar 

  • Verde, C., Malorni, A., and Parente, A. (1988).Ital. J. Biochem. 37, 329A-330A.

    Google Scholar 

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This paper is dedicated to the memory of Mr. Maurice Rees.

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Verde, C., Malorni, A. & Parente, A. The primary structure of papaya mosaic virus coat protein: A revision. J Protein Chem 8, 795–805 (1989). https://doi.org/10.1007/BF01024903

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