Summary
An efficient and reliable protocol for tomato protoplast isolation, culture, and plant regeneration has been developed. Fourteen diverse cultivars were tested. Fertile plants were regenerated from all 14 cultivars without any modification in the protocol. Plating efficiency (percentage of the protoplasts that formed minicalli) of up to 50% was achieved. Those mini-calli rapidly regenerated shoots at high frequencies. Regenerated shoots can be easily rooted on a basal medium with the appropriate auxin, and have been set to soil within two months after the isolation of the protoplasts.
Similar content being viewed by others
References
Behki RM, Lesley SM (1976)In vitro plant regeneration from leaf expiants ofLycoperison esculentum (tomato). Can J Bot 54:2409–2414
Bhatt DP, Fassuliotis G (1981) Plant regeneration from mesophyll protoplasts of eggplant. Z Pflanzenphysiol 104:81–89
Binding H (1974) Regeneration of haploid and dihaploid plants from protoplasts ofPetunia hybrida L. Z Pflanzen-physiol 74:327–356
Bingham ET, Hurley LV, Kaatz DM, Saunders JW (1975) Breeding alfalfa which regenerates from callus tissue in cultures. Crop Sci 15:719–721
Fobes JF (1980) The tomato as a model system for the molecular biologist. PMB Newslett 1:64–68
Morgan A, Cocking EC (1982) Plant regeneration from protoplasts ofLycopersicon esculentum Mill. Z Pflanzenphysiol 106:97–104
Mühlbach HP (1980) Different regeneration potentials of mesophyll protoplasts from cultivated and a wild species of tomato. Planta 148:89–96
Rick CM (1973) Potential genetic resources in tomato species: clues from observation in native habitats. In: Srb AM (ed) Handbook of genetics, vol 2. Plenum Press, New York, pp 255–269
Rick CM (1978) The tomato. Sci Am 239:76–87
Rick CM (1982) The potential of exotic germplasm for the tomato improvement In: Vasil I, Scowcroft W, Frey K (eds) Plant improvement and somatic cells genetics. Academic Press, London New York, pp 1–28
Shepard JF, Totten RE (1975) Isolation and regeneration of tobacco mesophyll cell protoplasts under low osmotic conditions. Plant Physiol 55:689–694
Thomas BR, Pratt D (1981) Breeding tomato strains for use in cell culture research. PMB Newslett 2:102–105
Zapata FJ, Sink KC, Cocking EC (1981) Calus formation from leaf mesophyll protoplasts of threeLycopersicon species;L. esculentum, c.v. ‘Walter’,L. pimpinellifolium andL. hirsutum, F. glabratum. Plant Sci Lett 23:41–46
Author information
Authors and Affiliations
Additional information
Communicated by G. Melchers
Rights and permissions
About this article
Cite this article
Shahin, E.A. Totipotency of tomato protoplasts. Theoret. Appl. Genetics 69, 235–240 (1985). https://doi.org/10.1007/BF00662431
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00662431