Skip to main content
SpringerLink
Log in

The genome of the Mediterranean fruitflyceratitis capitata: Localization of molecular markers by in situ hybridization to salivary gland polytene chromosomes

  • Original Articles
  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

We hybridized cloned DNA segments to salivary gland polytene chromosomes of the medfly,Ceratitis capitata, and thus established molecular markers for 24 sites on 6 out of 10 autosomal arms. An additional marker identified a medfly repetitive element that hybridizes to approximately 100 autosomal sites as well as a granular network that is thought to represent theX chromosome. Some of the markers correspond to 9 characterized transcription units, while 17 remain anonymous; at least 3 of the latter are restriction fragment length polymorphism (RFLP) markers. The characterized transcription units document that chromosomal arm 5L ofC. capitata is homologous to theDrosophila melanogaster X chromosome, in agreement with previous inferences based on the extensive conservation of linkage groups in Diptera.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anonymous (1987) Medfly genetics. Informational Circular, Joint FA/IAEA Division, Vienna, Austria

  • Bedo DG (1982) Differential sex chromosome replication and dosage compensation in polytene trichogen cells ofLucillia cuprina (Diptera: Calliphoridae). Chromosoma 87: 21–32

    Google Scholar 

  • BedoDG (1986) Polytene and mitotic chromosome analysis inCeratitis capitata (Diptera: Tephritidae). Can J Gen Cytol 28: 180–188

    Google Scholar 

  • BedoDG (1987) Polytene chromosome mapping inCeratitis capitata (Diptera: Tephritidae). Genome 29: 598–611

    Google Scholar 

  • BedoDG, ZacharopoulouA (1988) Inter-tissue variability of the polytene chromosome banding. Trends Genet 4: 90–91

    Google Scholar 

  • BeverlySM, WilsonAC (1984) Molecular evolution inDrosophila and the higher Diptera. II. A time scale for fly evolution. J Mol Evol 21: 1–13

    Google Scholar 

  • BrownNH, KingDL, WilcoxM, KafatosFC (1989) Alternative splicing ofDrosophila integrinPS2α transcripts. Cell 59: 185–195

    Google Scholar 

  • ChurchGM, GilbertW (1984) Genomic sequencing. Proc Natl Acad Sci USA 81: 1991–1995

    Google Scholar 

  • FeinbergAP, VogelsteinB (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13

    Google Scholar 

  • FeinbergAP, VogelsteinB (1984) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity, addendum. Anal Biochem 137: 266–267

    Google Scholar 

  • FosterGG, WhittenMJ, KonovalovC, ArnoldSTA, MaffiG (1981) Autosomal genetic maps of the Australian Sheep Blowfly,Lucillia cuprina dorsalis R-D (Dipteral, Calliphoridae) and possible correlations with the linkage maps ofMusca domestica L, andDrosophila melanogaster (Mg). Genet Res 37: 55–69

    Google Scholar 

  • GarabedianMJ, ShirrasAD, BownesM, WensinkPC (1987) The nucleotide sequence of the gene coding forDrosophila melanogaster yolk protein 3. Gene 55: 1–8

    Google Scholar 

  • Gasperi G, Guglielmino CR, Malacrida AR, Milani R (1991) Genetic variability and gene flow in geographic populations of the medfly,Ceratitis capitata. Heredity 67 (in press)

  • HarrisEJ (1989) Pest status of fruit flies. In: RobinsonAS, HooperGH (eds) Fruit flies, their biology, natural enemies and control. Elsevier, Amsterdam, pp 73–81

    Google Scholar 

  • HatzopoulosP, KambysellisMP (1987) Isolation and structural analysis ofDrosophila grimshawi vitellogenin genes. Mol Gen Genet 206: 475–484

    Google Scholar 

  • HaymerDS, AntleinerJE, HeM, ThanaphumS, SaulSH, IvyJ, HoutchensK, ArcangeliL (1990) Actin genes in the Mediterranean fruit fly,Ceratitis capitata. Genetics 125: 155–160

    Google Scholar 

  • HungM-C, WensinkPC (1983) Sequence and structure conservation in yolk proteins and their genes. J Mol Biol 164: 481–492

    Google Scholar 

  • KerremansPh, BourtzisK, ZacharopoulouA (1990) Cytogenetic analysis of three genetic sexing strains ofCeratitis capitata. Theor Appl Genet 80: 177–182

    Google Scholar 

  • KonsolakiM, KomitopoulouK, ToliasPP, KingDL, SwimmerC, KafatosFC (1990) The chorion genes of the medfly,Ceratitis capitata. I. Structural and regulatory conservation of thes36 gene relative to twoDrosophila species. Nucleic Acids Res 18: 1731–1737

    Google Scholar 

  • LoukasM, KafatosFC (1986) The actin loci in the genusDrosophila: establishment of chromosomal homologies among distantly related species by in situ hybridization. Chromosoma 94: 297–308

    Google Scholar 

  • LoukasM, KafatosFC (1988) Chromosomal locations of actin genes are conserved between themelanogaster andobscura groups ofDrosophila. Genetica 76: 33–41

    Google Scholar 

  • MalacridaA, GasperiG, BiscaldiGF, MilaniR (1984) Functional significance of gene clusters in the housefly,Musca domestica and in other Diptera. Atti 2nd Congr Nat Soc Ital Ecol SItE 5: 307–310

    Google Scholar 

  • MalacridaA, GasperiG, BiscaldiGF, MilaniR (1986) Persistence of linkage relationships among enzyme loci in some Dipteran species. Atti Assoc Genet Ital 31: 121–122

    Google Scholar 

  • ManiatisT, FritschEF, SambrookJ (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  • Martínez-CruzadoJ-C, SwimmerC, FenerjianMG, KafatosFC (1988) Evolution of the autosomal chorion locus inDrosophila I: General organization of the locus and sequence comparisons of geness15 ands19 in evolutionarily distant species. Genetics 119: 663–667

    Google Scholar 

  • MiklosGLG, YamamotoMT, DaviesJ, PirrottaV (1988) Micro-cloning reveals a high frequency of repetitive sequences characteristic of chromosome 4 and the β-heterochromatin ofDrosophila melanogaster. Proc Natl Acad Sci USA 85: 2051–2055

    Google Scholar 

  • RinaM, SavakisC (1991) A cluster of vitellogenin genes in the Mediterranean fruit flyCeratitis capitata: sequence and structural conservation in Dipteran yolk proteins and their genes. Genetics 127: 769–778

    Google Scholar 

  • RobinsonAS, VanHeemertC (1982)Ceratitis capitata — a suitable case for genetic sexing. Genetica 58: 224–232

    Google Scholar 

  • RösslerY (1979) Automated sexing ofCeratitis capitata: the development of strains with inherited, sex-limited pupal colour dimorphism. Entomophaga 24: 411–416

    Google Scholar 

  • SaulSH (1985) Diagnosing the heterogametic sex in the Mediterranean fruitfly (Diptera: Tephritidae): The first sex-linked gene. Ann Entomol Soc Am 78: 198–200

    Google Scholar 

  • SaulSH, RösslerY (1984) Genetic markers of the autosomal linkage groups of the Mediterranean fruitflyCeratitis capitata (Diptera: Tephritidae). Ann Entomol Soc Am 77: 323–327

    Google Scholar 

  • SaundersRDC, GloverDM, AshburnerM, Sidén-KiamosI, LouisC, MonastiriotouM, SavakisC, KafatosFC (1989) PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning. Nucleic Acids Res 17: 9027–9029

    Google Scholar 

  • SpradlingAC, MahowaldAP (1980) Amplification of genes for chorion proteins during oogenesis inDrosophila melanogaster. Proc Natl Acad Sci USA 77: 1096–1100

    Google Scholar 

  • SteinemannM (1982) Analysis of chromosomal homologies between two species of the subgenusSophophora: D. miranda andD. melanogaster using cloned DNA segments. Chromosoma 87: 77–88

    Google Scholar 

  • SteinemannM, PinskerW, SperlichD (1984) Chromosomal homologies within theDrosophila obscura group probed by in situ hybridization. Chromosoma 91: 46–53

    Google Scholar 

  • SturtevantAH, NovitskiE (1941) The homologies of the chromosome elements in the genusDrosophila. Genetics 26: 517–541

    Google Scholar 

  • ToliasPP, KonsolakiM, KomitopoulouK, KafatosFC (1990) The chorion genes of the medfly,Ceratitis capitata. II. Characterization of three novel cDNA clones obtained by differential screening of an ovarian library. Dev Biol 140: 105–112

    Google Scholar 

  • WhitingJHJr, PlileyMD, FarmerJL, JefferyDE (1989) In situ hybridization analysis of chromosomal homologies inDrosophila melanogaster andDrosophila virilis. Genetics 122: 99–109

    Google Scholar 

  • YanYL, KunertCJ, PostlethwaitJH (1987) Sequence homologies among the three yolk polypeptide (Yp) genes inDrosophila melanogaster. Nucleic Acids Res 15: 67–85

    Google Scholar 

  • ZacharopoulouA (1987) Cytogenetic analysis of mitotic and salivary gland chromosomes in the medflyCeratitis capitata. Genome 29: 67–71

    Google Scholar 

  • ZacharopoulouA (1990) Polytene chromosome maps in the medflyCeratitis capitata. Genome 33: 184–197

    Google Scholar 

  • ZacharopoulouA, BourtzisK, KerremansPh (1991 a) A comparison of polytene chromosomes in salivary glands and orbital bristle trichogen cells inCeratitis capitata. Genome 34: 215–219

    Google Scholar 

  • Zacharopoulou A, Riva ME, Malacrida A, Gasperi G (1991 b) Cytogenetic characterization of a genetic sexing strain inCeratitis capitata. Genome 34 (in press)

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Division of Genetics, Cell and Developmental Biology, University of Patras, GR-Patras, Greece

    A. Zacharopoulou

  2. Department of Cellular and Development Biology, Harvard University, 16 Divinity Avenue, 02138, Cambridge, MA, USA

    M. Frisardi, P. Tolias & F. C. Kafatos

  3. Institute of Molecular Biology and Biotechnology-FORTH, P.O. Box 1527, GR-71110, Heraklion, Crete, Greece

    C. Savakis, A. S. Robinson, M. Konsolaki & F. C. Kafatos

  4. Department of Medical Sciences, University of Crete Medical School, GR-Heraklion, Crete, Greece

    C. Savakis

  5. Department of Biochemistry, Cell and Molecular Biology and Genetics, University of Athens, GR-15701, Athens, Greece

    K. Komitopoulou

Authors
  1. A. Zacharopoulou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Frisardi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Savakis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. S. Robinson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Tolias
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Konsolaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. K. Komitopoulou
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. F. C. Kafatos
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

by P.B. Moens

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zacharopoulou, A., Frisardi, M., Savakis, C. et al. The genome of the Mediterranean fruitflyceratitis capitata: Localization of molecular markers by in situ hybridization to salivary gland polytene chromosomes. Chromosoma 101, 448–455 (1992). https://doi.org/10.1007/BF00582839

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00582839

Keywords

Search

Navigation