Summary
The transposon encoding resistance to mercuric ions, Tn501, is 5.2 (±0.1)×106 daltons and is bounded by small inverted repeats. The restriction sites for the restriction endonucleases EcoRI, HindIII and SalGI have been mapped on the element.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barth, P.T., Grinter, N.J.: Map of plasmid RP4 derived by insertion of transposon C. J. molec. Biol. 113, 455–474 (1977)
Bennett, P.M., Richmond, M.H.: Translocation of a discrete piece of deoxy-ribonucleic acid carrying an amp gene between replicons in Escherichia coli. J. Bact. 126, 1–6 (1976)
Chandler, P.M., Krishnapillai, V.: Isolation and properties of recombination deficient mutants of Pseudomonas aeruginosa. Mutation Res. 23, 15–23 (1974)
Cohen, S.N., Chang, A.C.Y., Hsu, L.: Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc. nat. Acad. Sci. (Wash.) 69, 2110–2114 (1972)
Cornelis, G., Bennett, P.M., Grinsted, J.: Properties of pGC1, a lac plasmid originating in Yersinia enterocolitica 842. J. Bact. 127, 1058–1062 (1976)
Demerec, M., Adelberg, E.A., Clark, A.J., Hartman, P.E.: A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76 (1966)
Figurski, D., Meyer, R., Miller D.S., Helinski, D.R.: Generation in vitro of deletions in the broad host range plasmid RK2 using phage Mu insertions and a restriction endonuclease. Gene 1, 107–119 (1976)
Grinsted, J., Bennett, P.M., Richmond, M. H.: A restriction enzyme map of the R-factor RP1. Plasmid 1, 34–37 (1977)
Grinsted, J., Saunders, J.R., Ingram, L.C., Sykes, R.B., Richmond, M.H.: Properties of an R factor which originated in Pseudomonas aeruginosa 1822. J. Bact. 110, 529–537 (1972)
Hedges, R.W., Jacob, A.: Transposition of ampicillin resistance from RP4 to other replicons. Molec. gen. Genet. 132, 31–40 (1974)
Heffron, F., Rubens, C., Falkow, S.: Translocation of a plasmid DNA sequence which mediates ampicillin resistance: Molecular nature and specificity of insertion. Proc nat. Acad. Sci. (Wash.) 72, 3623–3627 (1975)
Kopecko, D.J., Cohen, S.N.: Site-specific recA-independent recombination between bacterial plasmids: Involvement of palindromes at the recombinational loci. Proc. nat. Acad. Sci. (Wash.) 72, 1373–1377 (1975)
Lai, C.J., Nathans, D.: Deletion mutants of simian virus 40 generated by enzymatic excision of DNA segments from the viral genome. J. molec. Biol. 89, 179–193 (1974)
Meyer, R., Figurski, D., Helinski, D.R.: Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molec. gen. Genet. 152, 129–135 (1977)
Murray, K., Murray, N.E.: Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J. molec. Biol. 98, 551–564 (1975)
Novick, R.P., Clowes, R.C., Cohen, S.N., Curtiss III, R., Datta, N., Falkow, S.: Uniform nomenclature for bacterial plasmids: a proposal. Bact. Rev. 40, 168–189 (1976)
Robinson, M.K., Bennett, P.M., Richmond, M.H.: Inhibition of TnA translocation by TnA. J. Bact. 129, 407–414 (1977)
Rubens, C., Heffron, F., Falkow, S.: Transposition of a plasmid deoxyribonucleic acid sequence that mediates ampicillin resistance: Independence from host rec functions and orientation of insertion. J. Bact. 128, 425–434 (1976)
So, M., Gill, R., Falkow, S.: The generation of a Co1E1-Apr cloning vehicle which allows detection of inserted DNA. Molec. gen. Genet. 142, 239–249 (1975)
Stanisich, V.A.: Interaction between an R factor and an element conferring resistance to mercuric ions in Pseudomonas aeruginosa. Molec. gen. Genet. 128, 201–212 (1974)
Stanisich, V.A., Bennett, P.M., Richmond M.H.: Characterization of a translocation unit encoding resistance to mercuric ions that occurs on a nonconjugative plasmid in Pseudomonas aeruginosa. J. Bact. 129, 1227–1233 (1977)
Tanaka, T., Weisblum, B.: Construction of a colicin E1-R-factor of deoxyribonucleic acid. J. Bact. 121, 354–362 (1975)
Thomas, M., Davis, R.W.: Studies on the cleavage of bacteriophage lambda DNA with EcoRI restriction endonuclease. J. molec. Biol. 91, 315–328 (1975)
Author information
Authors and Affiliations
Additional information
Communicated by W. Arber
Rights and permissions
About this article
Cite this article
Bennett, P.M., Grinsted, J., Choi, C.L. et al. Characterisation of Tn501, a transposon determining resistance to mercuric ions. Molec. Gen. Genet. 159, 101–106 (1978). https://doi.org/10.1007/BF00401753
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00401753