Abstract
In 248 strains representing 205 yeast species, changes in pH coupled to glucose addition were followed in unbuffered cell suspensions. Alkalinization of the external medium elicited by glucose, indicating a H+-glucose symport was observed in 34% of the strains, most of them belonging to the genera Rhodotorula, Hansenula and Candida. H+ uptake coupled to glucose transport was observed only after exhaustion of glucose in growth media. This observation was taken as an indication that, in general, the synthesis of H+-glucose symport is under the control of catabolite repression. Subsequently to the addition of glucose, in most yeasts (82%) acidification was observed. This ability is probably related to the creation of a proton-gradient across the plasma membrane and is generally distributed among yeasts.
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References
Alderman, B. & M. Höfer (1981) The active transport of monosaccharides by the yeast Metschnikovia reukaufii: Evidence for an electrochemical gradient of H+ across the cell membrane. Experiment. Mycol 5: 120–132
Barnett, J.A. & A. Sims (1976) Some physiological observations on the uptake of D-glucose and 2-deoxy-D-glucose by starving and exponentially-growing yeasts. Archiv. Microbiol. 111: 185–192
Deák, T. (1978) On the existence of H+-symports in yeasts. Archiv. Microbiol. 116: 205–211
Eddy, A.A. & J.A. Novacki (1971) Stoichiometrical proton and potassium ion movements accompanying the absorption of amino acids by the yeast Saccharomyces carlsbergensis. Biochem. J. 122: 701–711
Goffeau, A. & C.W. Slayman (1981) The proton-translocating ATPase of the fungal plasma membrane. Biochim. Biophys. Acta 639: 197–223
Höfer, M. & P.C. Misra (1978) Evidence for a proton/sugar symport in the yeast Rhodotorula gracilis (glutins). Biochem. J. 172: 15–22
Jaspers, H.T.A. & J.Van Steveninck (1977) Active transport of L-sorbose and 2-deoxy-D-galactose in Saccharomyces fragilis. Biochim. Biophys. Acta 469: 292–300
Kreger-Van Rij, N.J.W. (Ed) (1984) The Yeasts 3rd edn. Elsevier Science Publishers B.V., Amsterdam
Mitchell, P. (1967) Active transport and ion accumulation. In: M. Florkin & E.H. Stotz (Eds) Elsevier Publishing Company, Amsterdam. Comprehensive Biochemistry 22: 167–197
Schneider, R.P. & W.R. Wiley (1971) Regulation of sugar transport in Neurospora crassa. J. Bacteriol 106: 487–492
Seaston, A., C. Inkson & A.A. Eddy (1973) The absorption of protons with amino acids and carbohydrates by yeast. Biochem. J. 134: 1031–1043
Serrano, R. (1984) Plasma membrane ATPase of fungi and plants as a novel type of proton-pump. Current Topics in Cellular Regulation 23: 87–126
Sigler, K., A. Knotková & A. Kotyk (1981) Factors governing substrate-induced generation and extrusion of protons in the yeast Saccharomyces cerevisiae. Biochim. Biophys. Acta 643: 572–582
Spencer-Martins, I. & N.Van Uden (1985) Catabolite interconversion of glucose transport system in the yeast Candida wickerhamii. Biochim. Biophys. Acta 812: 168–172
West, I.C. (1970) Lactose transport coupled to proton movements in Escherichia coli. Biochem. Biophys. Res. Commun. 41: 655–661
Van Uden, N. (1967) Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition. Archiv. Microbiol. 58: 155–168
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Loureiro-Dias, M.C. Movements of protons coupled to glucose transport in yeasts. A comparative study among 248 yeast strains. Antonie van Leeuwenhoek 54, 331–343 (1988). https://doi.org/10.1007/BF00393524
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DOI: https://doi.org/10.1007/BF00393524