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Monoterpene hydrocarbon biosynthesis by isolated leucoplasts of Citrofortunella mitis

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Abstract

A plastid vesicle preparation isolated from exocarpium of young Citrofortunella mitis (calamondin) fruits was able to synthesise monoterpene hydrocarbons when incubated with isopentenyl pyrophosphate. The electron-microscope comparison between this organelle fraction and the various plastid classes present in the peel tissues has shown the structural identity between these plastid vesicles and the leucoplasts of the epithelial cells lining the secretory pockets. The monoterpene biosynthesis required the presence of dimethylallyl pyrophosphate, Mn2+ or Mg2+ and was increased by addition of 2-mercaptoethanol. Evidence is provided that the leucoplast vesicles act as a complete system in which occur all the successive steps involved in monoterpene hydrocarbon elaboration from isopentenyl pyrophosphate.

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Abbreviations

DMAPP:

dimethylallyl pyrophosphate

IPP:

isopentenyl pyrophosphate

Tricine:

N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl] glycine

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Authors and Affiliations

  1. Laboratoire de Physiologie Cellulaire Végétale, E.R.A. au C.N.R.S., no 403, Université de Bordeaux I, Avenue des Facultés, F-33405, Talence Cedex, France

    Michel Gleizes, Ginette Pauly, Jean-Pierre Carde, Anne Marpeau & Colette Bernard-Dagan

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  1. Michel Gleizes
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  2. Ginette Pauly
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  3. Jean-Pierre Carde
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  4. Anne Marpeau
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  5. Colette Bernard-Dagan
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Gleizes, M., Pauly, G., Carde, JP. et al. Monoterpene hydrocarbon biosynthesis by isolated leucoplasts of Citrofortunella mitis . Planta 159, 373–381 (1983). https://doi.org/10.1007/BF00393177

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  • DOI: https://doi.org/10.1007/BF00393177

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