Summary
Plastid (pt) DNA from the red alga Porphyridium aerugineum was purified by CsCI gradient centrifugation. An EcoRI library of the ptDNA was screened with a gene probe specific for the gene encoding the large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco EC 4.1.1.39) from spinach. A 5.8 kb EcoRI clone containing the LSU gene (rbcL) was isolated and the DNA sequence of the Porphyridium rbcL gene and its flanking regions was determined. An open reading frame was found 130 by downstream from the rbcL gene that shows homology to genes coding for the small subunit of Rubisco (rbcS) from higher plants and cyanobacteria. Both genes (rbcL + rbcS) are cotranscribed. Comparison of rbcL and rbcS sequences from Porphyridium, higher plants and cyanobacteria seems to reveal a remarkable evolutionary distance between the plastids of the red algae (rhodoplasts), chloroplasts and cyanobacteria.
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References
Bedbrook JR, Smith SM, Ellis RJ (1980) Nature 287:692–697
Curtis SE, Haselkorn R (1983) Proc Natl Acad Sci USA 80:1835–1839
Davies LG, Dibner MD, Battey JF (1986) Basic methods in molecular biology. Elsevier Scientific Publ, Amsterdam
Hunt IT, George DG, Barker WA (1985) BioSystems 18:223–240
Kaling M (1983) Inaugural Dissertation. Justus Liebig Universität Giessen, FRG
Kröger M, Kröger-Block A (1984) Nucleic Acids Res 12:113–120
Kuhsel M, Kowallik KV (1987) Mol Gen Genet 207:361–368
Li N, Cattolico RA (1987) Mol Gen Genet 209:343–247
Linne v Berg K-H, Kowallik KV (1988) BioSystems 21:239–247
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Margulis L (1981) Symbiosis in cell evolution, W.H. Freeman & Co, San Francisco
Newsman S, Cattolico RA (1988) Plant Mol Biol 11:821–831
Nierzwicki-Bauer SA, Curtis SE, Haselkorn R (1984) Proc Natl Acad Sci USA 81:5961–5965
Ohyama K, Fukuda H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H (1986) Nature 322:572–574
Palmer JD (1985) Annu Rev Genet 19:325–345
Perasso R, Baroin A, Qu LH, Bachellerie JP, Adoutte A (1989) Nature 339:142–144
Reith M, Cattolico RA (1986) Proc Natl Acad Sci USA 83:8599–8603
Ruf M, Kössel H (1988) FEBS Lett 240:41–44
Sanger F, Nicklen S, Coulsen AR (1977) Proc Natl Acad Sci USA 74:5463–5468
Schlösser UG (1982) Ber Dtsch Bot Ges 95:181–276
Shine J, Dalgarno L (1975) Nature 254:34–38
Shinozaki K, Sugiura M (1983) Nucleic Acids Res 11:6957–6964
Shinozaki K, Sugiura M (1985) Mol Gen Genet 200:27–32
Shinozaki K, Yamada C, Takahata N, Sugiura M (1983) Proc Natl Acad Sci USA 80:4050–4054
Shinozaki K, Ohme M, Tanaka M, Wakasuki T, Hayashida N, Matsubayashi T, Zaita T, Chungwongse J, Obokata J, Yamagushi-Shinozaki K, Ohto C, Torezawa K, Meng B, Sugita Y, Deno H, Kamogashira T, Yamada K, Kusuda J, Taikawa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Starnes SM, Lambert DH, Maxwell ES, Stevens SE jr, Porter RD, Shively JM (1985) FEBS Lett 28:165–169
Steinmüller K, Kaling M, Zetsche K (1983) Planta 159:308–313
Sugita M, Manzara T, Pichersky E, Cashmore A, Gruissem W (1987) Mol Gen Genet 209:247–256
Zurawski G, Perrot B, Bottomly W, Whitfield PR (1981) Nucleic Acids Res 9:3251–3269
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Valentin, K., Zetsche, K. The genes of both subunits of ribulose-1,5-bisphosphate carboxylase constitute an operon on the plastome of a red alga. Curr Genet 16, 203–209 (1989). https://doi.org/10.1007/BF00391478
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DOI: https://doi.org/10.1007/BF00391478