Abstract
We have investigated the effects of saline solutions exposed to stainless steel hypodermic needles on their ability to reduce voltage-dependent calcium currents in chick dorsal root ganglion neurons and rat cerebellar Purkinje cells. Salines exposed to needles with brass hubs, but not those with plastic hubs, for as little as 2–3 sec reduced calcium currents in both cell types. The amplitude of the response was exposure-dependent and reversible. Elemental analysis of the exposed salines using inductively-coupled-plasma atomic emission spectroscopy revealed that Cu and Zn (but not Cd, Cr, Co, Fe, Mn, Ni or Pb) were released from the brazed needles. The amount of Cu plus Zn released in 30 sec was estimated to be 12–26 μM, depending upon the specific needle examined. Longer exposures produced proportionately higher concentrations of the metals. Dose-response curves for Cu or Zn ions applied directly to cells confirmed that similar concentrations of these ions reduced neuronal calcium currents. Our results indicate that divalent ions released from stainless steel hypodermic needles with brass hubs can interfere with measurements of calcium currents. In addition the results contribute new information regarding potential physiological and pathological actions of copper and zinc ions in biological tissues.
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References
Bean BP (1989) Classes of calcium channels in vertebrate cells. Ann Rev Physiol 51: 367–384
Bowman PW (1987) Inductively coupled plasma emission spectroscopy — methodology, instrumentation, and performance. Wiley-Interscience, NY
Choi DW, Yokoyama M, Koh J (1988) Zinc neurotoxicity in cortical cell culture. Neuroscience 24: 67–79
Cooper RL, Goldman JM, Rehnberg GL, McElroy WK, Hein JF (1987) Effects of metal cations on pituitary hormone secretion in vitro. J Biochem Toxicol 2: 241–249
Hagiwara S, Takahashi K (1967) Surface density of calcium ions and calcium spikes in the barnacle muscle fiber membrane. J Gen Physiol 50: 583–601
Hockberger PE, Toselli M, Swandulla D, Lux HD (1989) A diacylglycerol analogue reduces neuronal calcium currents independently of protein kinase C acivation. Nature 338: 340–342
Hockberger PE, Tseng HY, Connor JA (1989) Development of rat cerebellar Purkinje cells: electrophysiological properties following acute isolation and in long-term culture. J Neuroscience 9: 2258–2271
Hofmann J, Michel R, Holm R, Zilkens J (1981) Corrosion behavior of stainless steel implants in biological media. Surface and Interface Analysis 3: 110–117
Merritt K, Brown SA, Sharkey NA (1984) The binding of metal salts and corrosion products to cells and proteins in vitro. J Biomed.Materials Res. 18: 1005–1015
Peters S, Koh J, Choi DW (1987) Zinc selectively blocks the action of N-methyl-D-aspartate on cortical neurons. Science 236: 589–592
Szerdahelyi P, Kasa P (1984) Histochemistry of zinc and copper. Int Rev Cytol 89: 1–33
Williams DF (1981) Electrochemical aspects of corrosion in the physiological environment. In: Williams DF (ed) Fundamantal Aspects of Biocompatibility. Volume 1, Chapter 2. CRC Press, Inc., Boca Raton, Florida
Winegar BD, Lansman JB (1990) Voltage-dependent block by zinc of single-calcium channels in mouse myotubes. J Physiol 425: 563–578
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Nam, S.C., Hockberger, P.E. Divalent ions released from stainless steel hypodermic needles reduce neuronal calcium currents. Pflugers Arch. 420, 106–108 (1992). https://doi.org/10.1007/BF00378649
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DOI: https://doi.org/10.1007/BF00378649