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Chromosomal localization of the major histocompatibility complex of the horse (ELA) by in situ hybridization

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Abstract

The first gene assignment to a horse chromosome is reported for equine leucocyte antigen (ELA), the major histocompatibility complex of the horse. A cloned DNA sequence derived from a class I gene of the porcine major histocompatibility complex was used as a probe for an in situ hybridization experiment. We present the regional localization of ELA, using this sequence, to equine chromosome 20g14-q22.

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References

  • Alexander, A. J., Bailey, E., and Woodward, J. G.: Analysis of the equine lymphocyte antigen system by Southern blot hybridization. Immunogenetics 25: 47–54, 1987

    Google Scholar 

  • Antczak, D. F., Barger, B., Bailey, E., Bernoco, D., Bull, R. W., Byrns, G. R., Guerin, G., Lazary, S., McClure, J., Mottoroni, V. D., Symons, R., Templeton, J., and Varewyck, H.: Joint report of the Third International Workshop on Lymphocyte Alloantigens of the Horse. Anim Genet 17: 363–373, 1986

    Google Scholar 

  • Bailey, E., Antczak, D. F., Bernoco, D., Bull, R. W., Fister, R., Guerin, G., Lazary, S., Mattews, S., McClure, J., Meyer, J., Mottironi, V. D., and Templeton, J.: Joint report of the Second Internation al Workshop on Lymphocyte Alloantigens of the Horse. Anim Blood Groups Biochem Genet 15: 123–132, 1984

    Google Scholar 

  • Bernoco, D., Antczak, D. F., Bailey, E., Bull, K., Bull, R. W., Byrns, G., Guerin, G., Lazary, S., McClure, J., Templeton, J., and Varewyck, H.: Joint report of the Fourth International Workshop on Lymphocyte Alloantigens of the Horse. Anim Genet 18: 81–94, 1987

    Google Scholar 

  • Bull, R. W.: Joint report of the First International Workshop on Lymphocyte Alloantigens of the Horse. Anim Blood Groups Biochem Genet 14: 119–137, 1983

    Google Scholar 

  • Feinberg, A. P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983

    Google Scholar 

  • Feinberg, A. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266–267, 1984

    Google Scholar 

  • Ford, C. E., Pollock, D. L., and Gustavsson, I. (eds.): Proceedings of the first international conference for the standardization of banded karyotypes of domestic animals. Hereditas 92: 145–162, 1980

  • Fries, R., Hediger, R., and Stranzinger, G.: Tentative chromosomal localization of the bovine major histocompatibility complex by in situ hybridization. Anim Genet 17: 287–294, 1986

    Google Scholar 

  • Guerin, G., Bertaud, M., Chardon, P., Geffrotin, C., Vaiman, M., and Cohen, D.: Molecular genetic analysis of the major histocompatibility complex in an ELA typed horse family. Anim Genet 18: 323–336, 1987

    Google Scholar 

  • Halldorsdottir, S., and Davies, B.: Restriction fragment length polymorphism (RFLP) in Icelandic horses observed by using human MHC class II probes. Anim Genet 18: 29–30, 1987

    Google Scholar 

  • Hansen, K. M.: The relative length of Q-band/Giemsa stained horse chromosomes. Sixth Eur Colloq Cytogenet Domest Anim, pp. 165–171, 1984

  • Lin, C. C., Draper, P. N., and De Braekeeler, M.: High-resolution chromosomal localization of the β-gene of the human β-globin gene complex by in situ hybridization. Cytogenet Cell Genet 39: 269–274, 1985

    Google Scholar 

  • Rabin, M., Fries, R., Singer, D., and Ruddle, F. H.: Assignment of the porcine major histocompatibility complex to chromosome 7 by in situ hybridization. Cytogenet Cell Genet 39: 206–209, 1985

    Google Scholar 

  • Singer, D. S., Camerini-Otero, R. D., Satz, M. L., Osborne, B., Sachs, D., and Rudikoff, S.: Characterization of a porcine genomic clone encoding a major histocompatibility complex antigen: expression in mouse L cells. Proc Natl Acad Sci USA 79: 1403–1407, 1982

    Google Scholar 

  • Vaiman, M., Chardon, P., and Cohen, D.: DNA polymorphism in the major histocompatibility complex of man and various farm animals. Anim Genet 17: 113–133, 1986

    Google Scholar 

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Ansari, H.A., Hediger, R., Fries, R. et al. Chromosomal localization of the major histocompatibility complex of the horse (ELA) by in situ hybridization. Immunogenetics 28, 362–364 (1988). https://doi.org/10.1007/BF00364235

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  • DOI: https://doi.org/10.1007/BF00364235

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